GapMind for catabolism of small carbon sources

 

Protein WP_038857023.1 in Cronobacter universalis NCTC 9529

Annotation: NCBI__GCF_001277175.1:WP_038857023.1

Length: 892 amino acids

Source: GCF_001277175.1 in NCBI

Candidate for 19 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism ald-dh-CoA hi aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 95% 100% 1679.1
2'-deoxyinosine catabolism ald-dh-CoA hi aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 95% 100% 1679.1
2-deoxy-D-ribose catabolism ald-dh-CoA hi aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 95% 100% 1679.1
ethanol catabolism ald-dh-CoA hi aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 95% 100% 1679.1
ethanol catabolism etoh-dh-nad hi aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 95% 100% 1679.1 aldehyde dehydrogenase/alcohol dehydrogenase (EC 1.2.1.57) 59% 1047.7
L-threonine catabolism ald-dh-CoA hi aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 95% 100% 1679.1
thymidine catabolism ald-dh-CoA hi aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 95% 100% 1679.1
L-tryptophan catabolism ald-dh-CoA hi aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 95% 100% 1679.1
4-hydroxybenzoate catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 56% 100% 986.5 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
2'-deoxyinosine catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 56% 100% 986.5 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
2-deoxy-D-ribose catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 56% 100% 986.5 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
ethanol catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 56% 100% 986.5 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
L-threonine catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 56% 100% 986.5 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
thymidine catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 56% 100% 986.5 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
L-tryptophan catabolism adh med aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 (characterized) 56% 100% 986.5 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
L-fucose catabolism fucO lo Lactaldehyde reductase (characterized, see rationale) 34% 99% 219.9 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
L-rhamnose catabolism fucO lo Lactaldehyde reductase (characterized, see rationale) 34% 99% 219.9 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
L-threonine catabolism tdh lo L-threonine dehydrogenase (EC 1.1.1.103) (characterized) 34% 91% 204.5 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1
glycerol catabolism dhaD lo alcohol dehydrogenase (EC 1.1.1.1); long-chain-alcohol dehydrogenase (EC 1.1.1.192) (characterized) 33% 94% 182.2 aldehyde-alcohol dehydrogenase; EC 1.1.1.1; EC 1.2.1.10 95% 1679.1

Sequence Analysis Tools

View WP_038857023.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MAVTNVAELNALVERVKKAQREYANFTQEQVDKIFRAAALAAADARIPLAKMAVAESGMG
IVEDKVIKNHFASEYIYNAYKDEKTCGILDEDDTFGTITIAEPIGIICGIVPTTNPTSTA
IFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAAGAPKDLIGWIDQPSVELSNA
LMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTPVVIDETADIKRAVASILMSKTF
DNGVICASEQSVIVVDSVYDAVRERFASHGGYLLQGAELKAVQNIILKNGGLNAAIVGQP
AVKIAEMAGINVPSSTKILIGEVSLVDESEPFAHEKLSPTLAMYRAKDFEDAVEKAEKLV
AMGGIGHTSCLYTDQDNQPARVTFFGDKMKTARILINTPASQGGIGDLYNFKLAPSLTLG
CGSWGGNSISENVGPKHLINKKTVAKRAENMLWHKLPKSIYFRRGSLPIALDEVITDGHK
RAMIVTDRFLFNNGYADQITSVLKAAGVETEVFFEVEADPTLSVVRKGAELMNSFKPDVI
IALGGGSPMDAAKIMWVMYEHPETHFEELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSG
TGSEVTPFAVVTDDETGQKYPLADYALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHALE
AYVSVLASEFSDGQALQALKLLKENLPASYHEGSKNPVARERVHSAATIAGIAFANAFLG
VCHSMAHKLGSQFHIPHGLANALLISNVIRYNANDNPTKQTAFSQYDRPQARRRYAEVAD
HLGLSAPGDRTAAKIEKLLAWLESIKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQ
CTGANPRYPLIAELKQILLDSFYGREYKEGAEAVKAEAAPVAKADKKAKKTV

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory