Alignments for a candidate for fruII-ABC in Cronobacter universalis NCTC 9529

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruII-ABC in Cronobacter universalis NCTC 9529

Align The fructose porter, FruA (fructose-1-P forming IIABC) (Delobbe et al. 1975) FruA is 39% identical to 4.A.2.1.1). fructose can be metabolized to Fru-1-P via this system as well as Fru-6-P by another PTS system (characterized)
to candidate WP_007699731.1 AFK65_RS13270 PTS fructose transporter subunit IIBC

Query= TCDB::P71012
         (635 letters)



>NCBI__GCF_001277175.1:WP_007699731.1
          Length = 565

 Score =  417 bits (1073), Expect = e-121
 Identities = 224/482 (46%), Positives = 317/482 (65%), Gaps = 23/482 (4%)

Query: 162 AAPAPA------GKGKILAVTACPTGIAHTFMAADALKEKAKELGVEIKVETNGSSGIKH 215
           AA APA      G  +I+AVTACPTG+AHTFMAA+A++ +AK+ G  +KVET GS G  +
Sbjct: 92  AAAAPAASANASGPKRIVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGN 151

Query: 216 KLTAQEIEDAPAIIVAADKQVEMERFKGKRVLQVPVTAGIRRPQELIEKAMNQDAPIYQG 275
            +T +E+  A  +IVAAD +V++ +F GK + +      +++  +  +KA+ +  P YQ 
Sbjct: 152 AITPEEVAQADLVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQEFDKAVAEAKP-YQP 210

Query: 276 SGGGSAASNDDEEAKGKSGSGIGNTFYKHLMSGVSNMLPFVVGGGILVAISFFWGIHSAD 335
           +G G+AA+  D++ +G +G       Y+HL++GVS MLP VV GG+L+A+SF +GI +  
Sbjct: 211 AGAGAAAT--DKKEQGGAGP------YRHLLTGVSYMLPMVVAGGLLIALSFVFGIEAFK 262

Query: 336 PNDPSYNTFAAALNFIGGDNALKLIVAVLAGFIAMSIADRPGFAPGMVGGFMATQANAGF 395
                  T AAAL  IGG +A  L+V VLAG+IA SIADRPG  PG++GG +A    +GF
Sbjct: 263 QE----GTLAAALMKIGGGSAFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGF 318

Query: 396 LGGLIAGFLAGYVVILLKKVFTFIPQSLDGLKPVLIYPLFGIFITGVLMQFVVNTPVAAF 455
           +GG+IAGFLAGYV   +      +PQS++ LKP+LI PLF   I G+ M +V+ TPVA  
Sbjct: 319 IGGIIAGFLAGYVAKAISGKVK-LPQSMEALKPILIIPLFSSLIVGLAMIYVIGTPVAKI 377

Query: 456 MNFLTNWLESLGTGNLVLMGIILGGMMAIDMGGPLNKAAFTFGIAMIDAGNYAPHAAIMA 515
           +  LT WL+S+GT N VL+G ILG MM  DMGGP+NKAA+ FG+ ++ +  YAP AAIMA
Sbjct: 378 LAGLTAWLQSMGTANAVLLGAILGAMMCTDMGGPVNKAAYAFGVGLLSSQVYAPMAAIMA 437

Query: 516 GGMVPPLGIALATTIFRNKFTQRDREAGITCYFMGAAFVTEGAIPFAAADPLRVIPAAVV 575
            GMVPPL + +AT + R KF +  +E G     +G  F++EGAIPFAA DP+RVIP  + 
Sbjct: 438 AGMVPPLAMGIATLVARRKFDKGQQEGGKAALVLGLCFISEGAIPFAARDPMRVIPCCIA 497

Query: 576 GAAVAGGLTEFFRVTLPAPHGGVFVAFITN--HPML-YLLSIVIGAVVMAIILGIVKKPV 632
           G A+ G ++      L APHGG+FV  I     P+L YLL+IV G+++  ++   +K+P 
Sbjct: 498 GGALTGAISMAIGAKLMAPHGGLFVLLIPGAISPVLGYLLAIVAGSLLAGLVYAFLKRPE 557

Query: 633 TE 634
           +E
Sbjct: 558 SE 559


Lambda     K      H
   0.320    0.137    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 834
Number of extensions: 45
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 635
Length of database: 565
Length adjustment: 37
Effective length of query: 598
Effective length of database: 528
Effective search space:   315744
Effective search space used:   315744
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources