Alignments for a candidate for galK in Cronobacter universalis NCTC 9529

GapMind for catabolism of small carbon sources

Alignments for a candidate for galK in Cronobacter universalis NCTC 9529

Align galactose kinase (characterized)
to candidate WP_007706919.1 AFK65_RS06245 galactokinase

Query= CharProtDB::CH_024146
         (382 letters)



>NCBI__GCF_001277175.1:WP_007706919.1
          Length = 382

 Score =  664 bits (1712), Expect = 0.0
 Identities = 327/382 (85%), Positives = 353/382 (92%)

Query: 1   MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRD 60
           MSLKEKT +LFA  FGYPAT  IQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCA RD
Sbjct: 1   MSLKEKTHALFAEKFGYPATTHIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAKRD 60

Query: 61  DRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGN 120
           DR+VRV+AADY+NQ DEFSLD PI+ H++ QW+NYVRGVVKHLQ R+  FGG D+VISGN
Sbjct: 61  DRRVRVVAADYDNQTDEFSLDEPILTHDSQQWSNYVRGVVKHLQQRDPGFGGADLVISGN 120

Query: 121 VPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISAL 180
           VPQGAGLSSSASLEVAVGTV + LYHL LDGAQIALNGQEAENQFVGCNCGIMDQLISAL
Sbjct: 121 VPQGAGLSSSASLEVAVGTVFRHLYHLSLDGAQIALNGQEAENQFVGCNCGIMDQLISAL 180

Query: 181 GKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQP 240
           GKKDHALLIDCRSLGTKAV MP+GVAVVIINSNFKRTLVGSEYNTRREQCETGARFF Q 
Sbjct: 181 GKKDHALLIDCRSLGTKAVPMPQGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFTQK 240

Query: 241 ALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHAS 300
           ALRDV++++FNAVAHELDPIVAKRVRH+LTENARTVEAA AL +GDL RMG+LMAESHAS
Sbjct: 241 ALRDVSLDQFNAVAHELDPIVAKRVRHVLTENARTVEAADALAKGDLSRMGKLMAESHAS 300

Query: 301 MRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQY 360
           MRDDFEITVPQIDTLVEIVK+VIGD+GGVRMTGGGFGGC+VAL+PE +VP VQ AV  QY
Sbjct: 301 MRDDFEITVPQIDTLVEIVKSVIGDQGGVRMTGGGFGGCVVALVPEAIVPEVQAAVEAQY 360

Query: 361 EAKTGIKETFYVCKPSQGAGQC 382
           EA+TGIKETFYVCKPS+GAG C
Sbjct: 361 EARTGIKETFYVCKPSEGAGLC 382


Lambda     K      H
   0.318    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 532
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 382
Length adjustment: 30
Effective length of query: 352
Effective length of database: 352
Effective search space:   123904
Effective search space used:   123904
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_007706919.1 AFK65_RS06245 (galactokinase)
to HMM TIGR00131 (galK: galactokinase (EC 2.7.1.6))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00131.hmm
# target sequence database:        /tmp/gapView.2103777.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00131  [M=388]
Accession:   TIGR00131
Description: gal_kin: galactokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
     6e-169  547.9   0.0   6.7e-169  547.7   0.0    1.0  1  NCBI__GCF_001277175.1:WP_007706919.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_001277175.1:WP_007706919.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  547.7   0.0  6.7e-169  6.7e-169       2     388 .]       5     382 .]       4     382 .] 0.99

  Alignments for each domain:
  == domain 1  score: 547.7 bits;  conditional E-value: 6.7e-169
                             TIGR00131   2 eevkkiFasaykekpdlvvraPGRvnliGehiDYndgsvlPlaidvdtlvavkerddknvsitlanadnklae 74 
                                           e+++ +Fa++++++++  ++aPGRvnliGeh+DYndg+vlP+aid++t++++++rdd++v++++a++dn+++e
  NCBI__GCF_001277175.1:WP_007706919.1   5 EKTHALFAEKFGYPATTHIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAKRDDRRVRVVAADYDNQTDE 77 
                                           678999******************************************************************* PP

                             TIGR00131  75 rkldlpldksevsdWanYvkgvlkvlqeRfnsvplGldivisgdvPtgaGLsssaalevavaavlknlgklel 147
                                           ++ld p+  ++ ++W+nYv+gv+k+lq+R + ++ G+d+visg+vP+gaGLsssa+levav++v ++l++l l
  NCBI__GCF_001277175.1:WP_007706919.1  78 FSLDEPILTHDSQQWSNYVRGVVKHLQQRDPGFG-GADLVISGNVPQGAGLSSSASLEVAVGTVFRHLYHLSL 149
                                           **********************************.************************************** PP

                             TIGR00131 148 dskeillriqkveehfvGvncGgmDqlasvlGeedhallvefrkLkatpvklpqleialviantnvksnlaps 220
                                           d+++i+l++q++e++fvG+ncG+mDql+s+lG++dhall+++r+L +++v++pq ++a+vi+n+n+k++l++s
  NCBI__GCF_001277175.1:WP_007706919.1 150 DGAQIALNGQEAENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVPMPQ-GVAVVIINSNFKRTLVGS 221
                                           ******************************************************.****************** PP

                             TIGR00131 221 eYnlRrqeveeaakvlakksekgaLrDvkeeefaryearltkllqlvekqRakhvvsenlRvlkavkllkded 293
                                           eYn+Rr+++e++a+++++k    aLrDv+  +f+   a +++l+++v+k R++hv++en+R+++a+++l ++d
  NCBI__GCF_001277175.1:WP_007706919.1 222 EYNTRREQCETGARFFTQK----ALRDVSLDQFN---AVAHELDPIVAK-RVRHVLTENARTVEAADALAKGD 286
                                           *******************....***********...************.*********************** PP

                             TIGR00131 294 lkelGkLmnesqasldddyeitvpeidelvesialvnG.siGsRltGaGfGGCtvalvpnenvekvrkalaek 365
                                           l ++GkLm+es+as++dd+eitvp+id+lve+++ v+G ++G+R+tG+GfGGC+valvp+  v++v+ a+  +
  NCBI__GCF_001277175.1:WP_007706919.1 287 LSRMGKLMAESHASMRDDFEITVPQIDTLVEIVKSVIGdQGGVRMTGGGFGGCVVALVPEAIVPEVQAAVEAQ 359
                                           **************************************99********************************* PP

                             TIGR00131 366 Yekktdlklefavivskealgel 388
                                           Ye+ t++k++f+v++++e++g +
  NCBI__GCF_001277175.1:WP_007706919.1 360 YEARTGIKETFYVCKPSEGAGLC 382
                                           ********************977 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (388 nodes)
Target sequences:                          1  (382 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 16.00
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources