D-maltose catabolism in Cronobacter universalis NCTC 9529

GapMind for catabolism of small carbon sources

D-maltose catabolism in Cronobacter universalis NCTC 9529

Best path

Rules

Overview: Maltose utilization in GapMind is based on the MetaCyc pathway via maltose phosphorylase (link), or a phosphotransferase system followed by 6-phospho-alphaglucosidase, or a phosphotransferase system followed by a phosphatase back to maltose followed by maltose phosphorylase, or hydrolysis by alpha-glucosidase after uptake, or periplasmic hydrolysis by alpha-glucosidase followed by glucose utilization.

all:

maltose-transport, malP, pgmB and glk
or maltose-PTS, malA and glk
or maltose-PTS, mapP, malP, pgmB and glk
or maltose-transport, susB and glk
or susB and glucose-utilization
Comment: In the phosphorylase pathway, malP forms beta-glucose-1-phosphate and glucose, and beta-phosphoglucomutase forms glucose-6-phosphate, a central metabolite; glucokinase (glk) is also needed to feed the released glucose into central metabolism. Alternatively, PTS systems produce maltose 6'-phosphate, which can be cleaved by 6-phospho-alpha-glucosidase to glucose and glucose-6-phosphate. Alternatively, maltose can be hydrolyzed by alpha-glucosidase (EC 3.2.1.20) in the cytoplasm. Alternatively, maltose can be hydrolyzed by alpha-glucosidase (EC 3.2.1.20) in the periplasm, as in Bacteroides thetaiotaomicron, followed by glucose utilization.

glucose-utilization:

glucose-transport and glk
or glucose-PTS
or gdh, gnl, gadh1, gadh2, gadh3, kguT, kguK, kguD, edd and eda
Comment: Glucose can be taken up and then phosphorylated to glucose 6-phosphate by the kinase glk. Or, both uptake and phosphorylation are catalyzed by a PTS system. Or, glucose is oxidized to glucono-1,5-lactone in the periplasm (by gdh), hydrolyzed to gluconate (by gnl), oxidized to 2-ketogluconate (by gadh123), taken up by kguT, phosphorylated to 2-dehydro-6-phosphogluconate (by kguK), reduced to gluconate 6-phosphate (by kguD), dehydrated by edd to 2-dehydro-3-deoxy-gluconate 6-phosphate, and cleaved by aldolase eda to pyruvate and D-glyceraldehyde 3-phosphate.

glucose-PTS:

ptsG-crr
or bglF
or ptsG and crr
or manX, manY and manZ

glucose-transport:

MFS-glucose
or SSS-glucose
or glcU'
or PAST-A
or SemiSWEET
or SWEET1
or mglA, mglB and mglC
or gtsA, gtsB, gtsC and gtsD
or glcS, glcT, glcU and glcV
or aglE', aglF', aglG' and aglK'
Comment: Transporters and PTS systems were analyzed uzing query: transporter:glucose:D-glucose:D-glucopyranose:ALPHA-GLUCOSE:GLC:CPD-15374

maltose-PTS:

malEIICBA
or malEIICB and malEIIA
Comment: PTS systems form maltose 6'-phosphate

maltose-transport:

malE, malF, malG and malK
or thuE, thuF, thuG and thuK
or malE1, malF1, malG1 and malK1
or malX_Sm, malF_Sm, malG_Sm and malK_Sm
or musE, musF, musG, musK and musI
or malE_Aa, malF_Aa, malG_Aa and malK_Aa
or aglE, aglF, aglG and aglK
or malEF_Bb, malG_Bb and malK_Bb
or malE_Ss, malF_Ss, malG_Ss and malK_Ss
or MAL11
or SUC2
or SUT1
or cscB
or malAP
or malI
Comment: Transporters and PTS systems identified using: query: transporter:maltose:ALPHA-MALTOSE:CPD-15717

89 steps (56 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
malE	maltose ABC transporter, substrate-binding component MalE	AFK65_RS17615
malF	maltose ABC transporter, permease component 1 (MalF)	AFK65_RS17620	AFK65_RS18490
malG	maltose ABC transporter, permease component 2 (MalG)	AFK65_RS17625	AFK65_RS02840
malK	maltose ABC transporter, ATPase component MalK	AFK65_RS17610	AFK65_RS05695
susB	alpha-glucosidase (maltase)	AFK65_RS04850	AFK65_RS05705
glk	glucokinase	AFK65_RS14055	AFK65_RS04815
Alternative steps:
aglE	maltose ABC transporter, substrate-binding component AglK
aglE'	glucose ABC transporter, substrate-binding component (AglE)
aglF	maltose ABC transporter, permease component 1 (AglF)
aglF'	glucose ABC transporter, permease component 1 (AglF)
aglG	maltose ABC transporter, permease component 2 (AglG)	AFK65_RS05690	AFK65_RS17625
aglG'	glucose ABC transporter, permease component 2 (AglG)	AFK65_RS17625
aglK	maltose ABC transporter, ATPase component AglK	AFK65_RS17610	AFK65_RS05695
aglK'	glucose ABC transporter, ATPase component (AglK)	AFK65_RS17610	AFK65_RS05695
bglF	glucose PTS, enzyme II (BCA components, BglF)	AFK65_RS20275	AFK65_RS20435
crr	glucose PTS, enzyme IIA	AFK65_RS14175	AFK65_RS05935
cscB	maltose permease	AFK65_RS00545	AFK65_RS04425
eda	2-keto-3-deoxygluconate 6-phosphate aldolase	AFK65_RS11895	AFK65_RS00045
edd	phosphogluconate dehydratase	AFK65_RS11900	AFK65_RS00860
gadh1	gluconate 2-dehydrogenase flavoprotein subunit
gadh2	gluconate 2-dehydrogenase cytochrome c subunit	AFK65_RS09680
gadh3	gluconate 2-dehydrogenase subunit 3
gdh	quinoprotein glucose dehydrogenase	AFK65_RS03770	AFK65_RS04555
glcS	glucose ABC transporter, substrate-binding component (GlcS)
glcT	glucose ABC transporter, permease component 1 (GlcT)
glcU	glucose ABC transporter, permease component 2 (GlcU)
glcU'	Glucose uptake protein GlcU
glcV	glucose ABC transporter, ATPase component (GclV)	AFK65_RS18480	AFK65_RS06750
gnl	gluconolactonase	AFK65_RS14980
gtsA	glucose ABC transporter, substrate-binding component (GtsA)
gtsB	glucose ABC transporter, permease component 1 (GtsB)
gtsC	glucose ABC transporter, permease component 2 (GtsC)	AFK65_RS05690	AFK65_RS02840
gtsD	glucose ABC transporter, ATPase component (GtsD)	AFK65_RS10835	AFK65_RS05695
kguD	2-keto-6-phosphogluconate reductase	AFK65_RS18935	AFK65_RS00065
kguK	2-ketogluconokinase	AFK65_RS18925	AFK65_RS02370
kguT	2-ketogluconate transporter	AFK65_RS18930	AFK65_RS00060
MAL11	maltose permease
malA	6-phospho-alphaglucosidase	AFK65_RS00100	AFK65_RS14605
malAP	maltose permease
malE1	maltose ABC transporter, substrate-binding component (MalE1/MalE2)	AFK65_RS17615	AFK65_RS02830
malE_Aa	maltose ABC transporter, substrate-binding component	AFK65_RS17615
malE_Ss	maltose ABC transporter, substrate-binding component
malEF_Bb	maltose ABC transporter, fused substrate-binding and permease component 1
malEIIA	maltose phosphotransferase system, EII-A component (PtsG/YpqE/GamP)	AFK65_RS15470	AFK65_RS07765
malEIICB	maltose phosphotransferase system, EII-CB components curated:SwissProt::P54715	AFK65_RS15470	AFK65_RS07765
malEIICBA	maltose phosphotransferase system, EII-CBA components	AFK65_RS05935	AFK65_RS15470
malF1	maltose ABC transporter, permease component 1 (MalF1)	AFK65_RS17620
malF_Aa	maltose ABC transporter, permease component 1	AFK65_RS02835	AFK65_RS17620
malF_Sm	maltose ABC transporter, permease component 1	AFK65_RS02835	AFK65_RS17620
malF_Ss	maltose ABC transporter, permease component 1
malG1	maltose ABC transporter, permease component 2 (MalG1/MalG2)
malG_Aa	maltose ABC transporter, permease component 2	AFK65_RS02840	AFK65_RS17625
malG_Bb	maltose ABC transporter, permease component 2	AFK65_RS02840	AFK65_RS17625
malG_Sm	maltose ABC transporter, permease component 2	AFK65_RS02840	AFK65_RS10865
malG_Ss	maltose ABC transporter, permease component 2
malI	maltose transporter
malK1	maltose ABC transporter, ATPase component	AFK65_RS10835	AFK65_RS15775
malK_Aa	maltose ABC transporter, ATPase component	AFK65_RS10835	AFK65_RS05695
malK_Bb	maltose ABC transporter, ATPase component	AFK65_RS02825	AFK65_RS10835
malK_Sm	maltose ABC transporter, ATPase component	AFK65_RS10835	AFK65_RS18480
malK_Ss	maltose ABC transporter, ATPase component	AFK65_RS11165	AFK65_RS18835
malP	maltose phosphorylase
malX_Sm	maltose ABC transporter, substrate-binding component
manX	glucose PTS, enzyme EIIAB	AFK65_RS11735
manY	glucose PTS, enzyme EIIC	AFK65_RS11740
manZ	glucose PTS, enzyme EIID	AFK65_RS11745
mapP	maltose 6'-phosphate phosphatase
MFS-glucose	glucose transporter, MFS superfamily	AFK65_RS16050	AFK65_RS04760
mglA	glucose ABC transporter, ATP-binding component (MglA)	AFK65_RS13210	AFK65_RS19710
mglB	glucose ABC transporter, substrate-binding component	AFK65_RS13215	AFK65_RS19000
mglC	glucose ABC transporter, permease component (MglC)	AFK65_RS13205	AFK65_RS19705
musE	maltose ABC transporter, substrate-binding component MusE
musF	maltose ABC transporter, permease component 1 (MusF)
musG	maltose ABC transporter, permease component 2 (MusG)
musI	maltose ABC transporter, uncharacterized membrane component MusI
musK	maltose ABC transporter, ATPase component MusK	AFK65_RS18480	AFK65_RS02825
PAST-A	proton-associated sugar transporter A
pgmB	beta-phosphoglucomutase	AFK65_RS10840	AFK65_RS15220
ptsG	glucose PTS, enzyme IICB	AFK65_RS07765	AFK65_RS15470
ptsG-crr	glucose PTS, enzyme II (CBA components, PtsG)	AFK65_RS05470	AFK65_RS15470
SemiSWEET	Sugar transporter SemiSWEET
SSS-glucose	Sodium/glucose cotransporter
SUC2	maltose:H+ symporter
SUT1	maltose:H+ symporter
SWEET1	bidirectional sugar transporter SWEET1
thuE	maltose ABC transporter, substrate-binding component ThuE	AFK65_RS05680
thuF	maltose ABC transporter, permease component 1 (ThuF)	AFK65_RS05685	AFK65_RS10870
thuG	maltose ABC transporter, permease component 2 (ThuG)	AFK65_RS05690	AFK65_RS10865
thuK	maltose ABC transporter, ATPase component ThuK	AFK65_RS17610	AFK65_RS05695

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources