GapMind for catabolism of small carbon sources

 

xylitol catabolism in Cronobacter universalis NCTC 9529

Best path

PLT5, xdhA, xylB

Rules

Overview: Xylitol utilization in GapMind is based on the MetaCyc pathway via xylitol dehydrogenase (link) or on utilization via a phosphotransferase system and D-xylulose-5-phosphate 2-reductase (PMID:27553222).

19 steps (14 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
PLT5 xylitol:H+ symporter PLT5 AFK65_RS04490
xdhA xylitol dehydrogenase AFK65_RS02310 AFK65_RS14005
xylB xylulokinase AFK65_RS18990
Alternative steps:
Dshi_0546 xylitol ABC transporter, ATPase component AFK65_RS18480 AFK65_RS02825
Dshi_0547 xylitol ABC transporter, substrate-binding component
Dshi_0548 xylitol ABC transporter, permease component 1
Dshi_0549 xylitol ABC transporter, permease component 2 AFK65_RS05690 AFK65_RS02840
EIIA-Axl xylitol PTS, enzyme IIA (EIIA-Axl) AFK65_RS02325
EIIB-Axl xylitol PTS, enzyme IIB (EIIB-Axl) AFK65_RS02320
EIIC-Axl xylitol PTS, enzyme IIC (EIIC-Axl) AFK65_RS02315
fruI xylitol PTS, enzyme IIABC (FruI) AFK65_RS13270
HSERO_RS17000 xylitol ABC transporter, substrate-binding component
HSERO_RS17005 xylitol ABC transporter, permease component 1
HSERO_RS17010 xylitol ABC transporter, permease component 2
HSERO_RS17020 xylitol ABC transporter, ATPase component AFK65_RS18480 AFK65_RS10835
PS417_12055 xylitol ABC transporter, substrate-binding component AFK65_RS16910
PS417_12060 xylitol ABC transporter, permease component AFK65_RS19705 AFK65_RS12170
PS417_12065 xylitol ABC transporter, ATPase component AFK65_RS19710 AFK65_RS12175
x5p-reductase D-xylulose-5-phosphate 2-reductase AFK65_RS02310 AFK65_RS19280

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory