GapMind for catabolism of small carbon sources

 

Protein WP_053544305.1 in Corynebacterium deserti GIMN1.010

Annotation: NCBI__GCF_001277995.1:WP_053544305.1

Length: 345 amino acids

Source: GCF_001277995.1 in NCBI

Candidate for 15 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-maltose catabolism thuF med ABC transporter for D-Trehalose, permease component 1 (characterized) 44% 91% 240 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
sucrose catabolism thuF med ABC transporter for D-Trehalose, permease component 1 (characterized) 44% 91% 240 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
trehalose catabolism thuF med ABC transporter for D-Trehalose, permease component 1 (characterized) 44% 91% 240 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
D-glucosamine (chitosamine) catabolism SM_b21220 lo ABC transporter for D-Glucosamine, permease component 2 (characterized) 35% 93% 172.9 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
xylitol catabolism Dshi_0548 lo ABC transporter for Xylitol, permease component 1 (characterized) 31% 96% 131 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
glycerol catabolism glpP lo GlpP, component of Glycerol uptake porter, GlpSTPQV (characterized) 30% 99% 126.3 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
trehalose catabolism treT lo TreT, component of Trehalose porter (characterized) 32% 79% 125.6 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
lactose catabolism lacF lo ABC transporter for Lactose, permease component 1 (characterized) 30% 76% 109.4 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
D-cellobiose catabolism gtsB lo Glucose transport system permease protein aka TT_C0327, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 30% 63% 89 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
D-glucose catabolism gtsB lo Glucose transport system permease protein aka TT_C0327, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 30% 63% 89 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
lactose catabolism gtsB lo Glucose transport system permease protein aka TT_C0327, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 30% 63% 89 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
D-maltose catabolism gtsB lo Glucose transport system permease protein aka TT_C0327, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 30% 63% 89 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
D-mannose catabolism TT_C0327 lo Glucose transport system permease protein aka TT_C0327, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 30% 63% 89 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
sucrose catabolism gtsB lo Glucose transport system permease protein aka TT_C0327, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 30% 63% 89 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1
trehalose catabolism gtsB lo Glucose transport system permease protein aka TT_C0327, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 30% 63% 89 PalF, component of Platinose (isomaltulose) (6-O-α-D-glucopyranosyl-D-fructofuranose) porter 47% 251.1

Sequence Analysis Tools

View WP_053544305.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MANLKQARSAAWLIAPAMVVLAVVIGYPIVRAVWLSFQADKGLDPTTGLFTDGGFAGLDN
YLYWLTQRCMGADGTVRVCPPGTLATDFWPALRITLFFTVITVGLETILGICMALIMNKE
FRGRALVRAAVLIPWAIPTAVTAKLWQFIFAPQGIINSLFGLNISWTTDPWAARAAVILA
DVWKTTPFMALLILAGLQMIPKETYEAARVDGATAWQQFTKITLPLVRPALMVAVLFRTL
DALRMYDLPVIMISSSSNSPTAVISQLVVEDMRQNNFNSASALSTLIFLLIFFVAFIMIR
FLGADVSGQRGMKKKKLGGVRNPAKKPAPVRENRSLVSAKEDRAS

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory