GapMind for catabolism of small carbon sources

 

Protein WP_053545122.1 in Corynebacterium deserti GIMN1.010

Annotation: NCBI__GCF_001277995.1:WP_053545122.1

Length: 228 amino acids

Source: GCF_001277995.1 in NCBI

Candidate for 8 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-glutamate catabolism gluC hi GluC aka CGL1952, component of Glutamate porter (characterized) 97% 100% 424.5 Probable glutamine ABC transporter permease protein GlnM 34% 130.2
L-histidine catabolism Ac3H11_2554 lo ABC transporter for L-Histidine, permease component 1 (characterized) 32% 95% 106.3 GluC aka CGL1952, component of Glutamate porter 97% 424.5
L-glutamate catabolism gltJ lo Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida (characterized) 32% 84% 99.8 GluC aka CGL1952, component of Glutamate porter 97% 424.5
L-glutamate catabolism gluD lo GluD aka CGL1953, component of Glutamate porter (characterized) 32% 71% 94.7 GluC aka CGL1952, component of Glutamate porter 97% 424.5
L-citrulline catabolism PS417_17595 lo ABC transporter permease subunit; SubName: Full=Amino acid ABC transporter permease; SubName: Full=Histidine transport system permease protein (characterized, see rationale) 31% 94% 89 GluC aka CGL1952, component of Glutamate porter 97% 424.5
L-asparagine catabolism peb1D lo Amino acid ABC transporter, permease protein PEB1 (characterized, see rationale) 30% 85% 81.3 GluC aka CGL1952, component of Glutamate porter 97% 424.5
L-aspartate catabolism peb1D lo Amino acid ABC transporter, permease protein PEB1 (characterized, see rationale) 30% 85% 81.3 GluC aka CGL1952, component of Glutamate porter 97% 424.5
L-histidine catabolism BPHYT_RS24005 lo Polar amino acid ABC transporter, inner membrane subunit; Flags: Precursor (characterized, see rationale) 31% 84% 77.8 GluC aka CGL1952, component of Glutamate porter 97% 424.5

Sequence Analysis Tools

View WP_053545122.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSTLWADLGPSLLPAFWVTIKLTIYSAIGAMILGTILTAMRVSPVKILRTLSTAYINTVR
NTPLTLVVLFCSFGLYQNLGMTLAGRDSSTFLADNNFRLAVLGFILYTSTFVAESLRSGI
NTVHFGQAEAARSLGLGFGDTFRSIIFPQAVRAAIVPLGNTLIALTKNTTIASVIGVGEA
SLLMKSTIENHANMLFVVFSIFAVGFMILTLPMGLGLGKLSERLAVKK

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory