Alignments for a candidate for acn in Polaribacter dokdonensis DSW-5

GapMind for catabolism of small carbon sources

Alignments for a candidate for acn in Polaribacter dokdonensis DSW-5

Align Aconitate hydratase A; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 (characterized)
to candidate WP_053974641.1 I602_RS10500 aconitate hydratase

Query= SwissProt::Q937N8
         (869 letters)



>NCBI__GCF_001280865.1:WP_053974641.1
          Length = 755

 Score =  197 bits (501), Expect = 2e-54
 Identities = 215/763 (28%), Positives = 329/763 (43%), Gaps = 108/763 (14%)

Query: 74  PARVVCHDILGQTALVDLAGLRDAIADQGGDPAKVNPVVPVQLIVDHSLAVECGGFDPDA 133
           P R+ C D   Q AL+           Q G   K    VP  +  DH +  + G      
Sbjct: 61  PDRIACQDATAQMALLQFM--------QAG---KAKVAVPTTVHCDHLIQAKEGA----- 104

Query: 134 FAKNRAIEDRRNEDRFHFIDWTKQAFKNVDVIPPGNGIMHQINLEKMSPVIHADNGVAYP 193
            AK+    +  + + F+F++     +  +    PG GI+HQ+ LE            A+P
Sbjct: 105 -AKDLKHANNTSSEVFNFLESVSNKY-GIGFWKPGAGIIHQVVLENY----------AFP 152

Query: 194 D-TCVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIVGVELTGKRQPGITA 252
               +GTDSHT +   LG++AIGVGG +A +VM G A  ++ P ++GV+LTGK       
Sbjct: 153 GGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGKLSGWTAP 212

Query: 253 TDIVLALTEFLRKEKVVGAYLEFRGEGASSLTLGDRATISNMAPEYGATAAMFFIDEQTI 312
            D++L + E L  +   GA +E+ G GA+S++   + TI NM  E GAT + F  DE   
Sbjct: 213 KDVILKVAEILTVKGGTGAIVEYFGPGATSMSCTGKGTICNMGAEIGATTSTFGYDESME 272

Query: 313 DYLRLTGRTD--EQLKLVETYARTAGLWADSLKNAE--YERVLKFDLSSVVRNMAGPSNP 368
            YL+ T R D       ++ Y       A+  +N E  +++V++ +LS +   + GP  P
Sbjct: 273 RYLKATDREDVANAANEIKEYLTAD---AEVYENPEQYFDQVIEINLSELGPLLNGPFTP 329

Query: 369 HKRLPTSALAERGIAVDLDKASAQEAEGLMPDGAVIIAAITSCTNTSNPRNVIAAALLAR 428
              L T+  +E       +KA+A +    +  G      I SCTN+S   ++  A+ +A+
Sbjct: 330 D--LSTTVGSEM-----TEKANANDWPLTVEWG-----LIGSCTNSSY-EDLSRASSIAQ 376

Query: 429 NANARGLARKPWVKSSLAPGSKAVELYLEEANLLPDLEKLGFGIVAFACTTCNGM-SGAL 487
            A  +GL  K   +  + PGS+ V    E   +L   E L   I   AC  C G  +   
Sbjct: 377 QAIDKGLKTK--AEFGINPGSEQVRYTAERDGILQIFENLDAKIFTNACGPCIGQWARYS 434

Query: 488 DPKIQQEIIDRDLYATAVLSGNRNFDGRIHPYAK-QAFLASPPLVVAYAIAGTIRFDIEK 546
           DPK   +        + V S NRNF  R        AF+ASP +  A AIAG + F+  K
Sbjct: 435 DPKNAPK-------NSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLK 487

Query: 547 DVLGTDQDGKPVYLKDIWPSDEEIDAIVAKSVKPEQFRKVYEPMFAITAASGESVSPLYD 606
           D L  ++DG+ V   +  P+  E+         P +  +V E  +      G  V    +
Sbjct: 488 DKL-INEDGEEVMFDE--PTGWEL---------PPKGFEVKENGYLAPDEDGSGVVVSVN 535

Query: 607 WRPQSTYIRRPPYWEGALAGERTLKALRPLAVLGDNITTDHLSPSNAIMLNSAAGEYLAR 666
              +   +  P    G+     T+   + L       TTDH+         S AG +L  
Sbjct: 536 DDSERLQLLTPFTPLGS-----TITGAKLLIKAFGKCTTDHI---------SMAGPWLRF 581

Query: 667 MGLPEEDFNSYATHRGDHLTAQRATFANPTLINEMAVVDGQVKKGSLARIEPEGKVVRMW 726
            G               HL       AN TLI     V+   KK +  + +  G+   + 
Sbjct: 582 RG---------------HLD----NIANNTLI---GAVNAFNKKTNFVKNQLTGEYDAVP 619

Query: 727 EAIETYMDRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEVIVAEGFERIHRTNLIGMGV 786
                Y       I++   +YG+GSSR+ AA   R  GV  ++ + F RIH TNL   G+
Sbjct: 620 AVQREYKKEGIKTIVVGDHNYGEGSSREHAAMQPRHLGVAAVIVKSFARIHETNLKKQGM 679

Query: 787 LPLEFKPGVNRLTLGLDGTETYDVIGERQPRATLTLVVNRKNG 829
           L L F    +   +  D T  +  + E  P   LTL +  K+G
Sbjct: 680 LGLTFANEADYDLIQEDDTFNFTDLNEFAPDKQLTLEIVHKDG 722


Lambda     K      H
   0.318    0.135    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1517
Number of extensions: 71
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 869
Length of database: 755
Length adjustment: 41
Effective length of query: 828
Effective length of database: 714
Effective search space:   591192
Effective search space used:   591192
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources