GapMind for catabolism of small carbon sources

 

Alignments for a candidate for bamI in Amantichitinum ursilacus IGB-41

Align Iron-sulfur cluster-binding protein, putative (characterized, see rationale)
to candidate WP_053936339.1 WG78_RS03185 NADH-quinone oxidoreductase subunit G

Query= uniprot:Q39TW6
         (218 letters)



>NCBI__GCF_001294205.1:WP_053936339.1
          Length = 768

 Score = 94.7 bits (234), Expect = 4e-24
 Identities = 65/204 (31%), Positives = 95/204 (46%), Gaps = 30/204 (14%)

Query: 4   INLQIDGKEVVATEGMTILDAAKSVGISIPTLCHHEKLEPYGGCRICTVEVEVRGWPKLV 63
           + ++IDGK++    G T++DAA SVG  IP  C+H+KL     CR+C V+VE    PK +
Sbjct: 2   LEVEIDGKKLTVPGGSTVMDAANSVGAYIPHFCYHKKLSIAANCRMCLVQVEKA--PKPL 59

Query: 64  AGCIYPVEKGLVVRTRNEKIDKIRKVLLEEMLAHAP---------DSEELKALAQEYGAD 114
             C  PV  G+ V T ++   K +  ++E +L + P            +L+ LA  YG  
Sbjct: 60  PACATPVTDGMKVWTHSDSAVKAQAGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGKS 119

Query: 115 RDRFEKHP----------------SFCIHCGLCVRYCAEIKKKNAVGFVDRGSNREI-SF 157
             R+E+                  + CIHC  CVR+  E+     +G   RG + EI SF
Sbjct: 120 ASRYEEEKRSVVNKNLGPLISTDMTRCIHCTRCVRFTEEVAGYQELGMPGRGEHSEIMSF 179

Query: 158 IPEIAAKECWDCKECFPLCPTSAL 181
           I +    E         LCP  AL
Sbjct: 180 IGKTVNSEI--SGNVIDLCPVGAL 201


Lambda     K      H
   0.320    0.137    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 313
Number of extensions: 12
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 218
Length of database: 768
Length adjustment: 31
Effective length of query: 187
Effective length of database: 737
Effective search space:   137819
Effective search space used:   137819
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory