Align D-galactarolactone cycloisomerase (EC 5.5.1.27) (characterized)
to candidate WP_053937448.1 WG78_RS08815 L-rhamnonate dehydratase
Query= BRENDA::A9CEQ8 (378 letters) >NCBI__GCF_001294205.1:WP_053937448.1 Length = 394 Score = 143 bits (360), Expect = 9e-39 Identities = 107/346 (30%), Positives = 165/346 (47%), Gaps = 32/346 (9%) Query: 32 VLVEIECDDGTVGWGECLGPARPNAAVVQAYSGWLIGQDPRQTEKIWAVLYNALRDQGQR 91 ++VE+E DGTVG+ G V + + ++ GQ EKIW +Y + G++ Sbjct: 59 LVVEVEASDGTVGFAVTTGGEIGAYIVEKHLARFVEGQLVTDIEKIWDQMYFSTLYYGRK 118 Query: 92 GLSLTALSGIDIALWDIKGKHYGASISMLLGGRWRESVRAYATGSFKRDNVDRVSDNASE 151 G+ + +SG+D+ALWD+ K G + LLGG RE ++ YATG+ D A E Sbjct: 119 GVVINTISGVDLALWDLLAKVRGEPVHQLLGGAVREELQFYATGA--------RPDLAKE 170 Query: 152 MAERRAEGFHACKIKIGF-------GVEEDLRVIAAVREAIGPDMRLMIDANHGYTVTEA 204 M GF K+ + G+ ++L +IA +R +G D LM+D V A Sbjct: 171 M------GFIGGKMPLHHGPAEGEAGLRKNLEMIADMRNRVGDDFWLMLDCWMSLDVNYA 224 Query: 205 ITLGDRAAGFGIDWFEEPVVPEQLDAYARVRAGQP--IPVAGGETWHGRYGMWQALSAGA 262 L A +G+ W EE + P+ YA ++ P + V GE R+G L Sbjct: 225 TRLATAAHAYGLKWIEEALPPDDYWGYAELKRNVPRGMLVTTGEHEATRWGFRLLLEQQC 284 Query: 263 VDILQPDLCGCGGFSEIQKIATLATLHGVRIVPHVWGTGVQIAAALQFMAAMTPDPVRVN 322 DI+QPD+ CGG +E+ KI+ LA H V +VPH G+ V + F+ P Sbjct: 285 ADIIQPDVGWCGGMTELIKISALADAHNVMVVPH--GSSVY---SYHFVITRHNSPF-AE 338 Query: 323 PIEPIMEFDRTHNPFRQAVLREPLEAVNGVVTIPD--GPGLGIEIN 366 + + D+ F +L EP+ VNG + + + PG G+ +N Sbjct: 339 FLMMAPKADKVVPMFNPLLLDEPV-PVNGRIKVSELQAPGFGVRLN 383 Lambda K H 0.321 0.138 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 427 Number of extensions: 21 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 378 Length of database: 394 Length adjustment: 30 Effective length of query: 348 Effective length of database: 364 Effective search space: 126672 Effective search space used: 126672 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory