GapMind for catabolism of small carbon sources

 

Alignments for a candidate for BPHYT_RS24015 in Amantichitinum ursilacus IGB-41

Align ABC transporter related (characterized, see rationale)
to candidate WP_053938866.1 WG78_RS16145 amino acid ABC transporter ATP-binding protein

Query= uniprot:B2TBJ9
         (263 letters)



>NCBI__GCF_001294205.1:WP_053938866.1
          Length = 242

 Score =  216 bits (550), Expect = 4e-61
 Identities = 118/247 (47%), Positives = 161/247 (65%), Gaps = 11/247 (4%)

Query: 9   LSVKNIHKSFGDHHVLKGISLDAHQGDVISILGASGSGKSTFLRCLNLLETPDDGSVSLA 68
           +S+KNI K +GD  VL   S +  +G+V+ + G SGSGKST ++C+N LE    G + + 
Sbjct: 2   ISIKNISKWYGDFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLIKCVNGLEAFQKGDIIVD 61

Query: 69  GEELKMKRRGDGKLQPSDRRQVDRVRSQLGMVFQNFNLWSHMTVLENLIEGPMRVQKRSR 128
           G  +     GD K        + ++RS++GMVFQNF L+ H+++ +NL    M+V  R+R
Sbjct: 62  GTSV-----GDPKTN------LPKLRSRVGMVFQNFELFPHLSITQNLTLAQMQVLGRNR 110

Query: 129 AESVEEAEALLAKVGLAEKRGHYPAHLSGGQQQRVAIARALAMHPKVMLFDEPTSALDPE 188
            E+      LL +VGL      +P  LSGGQQQRVAIARALAM P  MLFDEPTSALDPE
Sbjct: 111 DEATVNGLRLLDRVGLKAHADKHPGQLSGGQQQRVAIARALAMDPIAMLFDEPTSALDPE 170

Query: 189 LVGEVLRVMRSLAEEGRTMLVVTHEMGFARHVSNRVMFLHQGQVEADGTPDEVFVECKSD 248
           +V EVL VM  LA+EG TM+ VTHEMGFAR V+NRV+F+ +G +  D T ++ F   +S+
Sbjct: 171 MVNEVLDVMVELAQEGMTMMCVTHEMGFARKVANRVIFMDKGAIVEDATKEDFFGSPRSE 230

Query: 249 RFRQFVS 255
           R + F+S
Sbjct: 231 RAQVFLS 237


Lambda     K      H
   0.318    0.133    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 162
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 263
Length of database: 242
Length adjustment: 24
Effective length of query: 239
Effective length of database: 218
Effective search space:    52102
Effective search space used:    52102
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory