GapMind for catabolism of small carbon sources

 

Alignments for a candidate for LRA5 in Amantichitinum ursilacus IGB-41

Align 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD(+)); 2-keto-3-deoxy-L-rhamnonate dehydrogenase; KDRDH; L-KDR dehydrogenase; L-KDR 4-dehydrogenase; EC 1.1.1.401 (characterized)
to candidate WP_053939027.1 WG78_RS16965 acetoacetyl-CoA reductase

Query= SwissProt::Q1NEI6
         (249 letters)



>NCBI__GCF_001294205.1:WP_053939027.1
          Length = 244

 Score =  133 bits (334), Expect = 4e-36
 Identities = 85/243 (34%), Positives = 137/243 (56%), Gaps = 7/243 (2%)

Query: 11  RCAIVTGGASGLGKQVAARIIAEGGAVAL-WDLNGDA---LAATQAEIDATHVVALDVSD 66
           R A+VTGG  G+G  +  ++   G  VA  +   G     LA   A   A H    +V+D
Sbjct: 3   RLALVTGGMGGIGTAICRQLADAGYQVATTYSRTGRQEQWLADATAAGYAFHAFECNVAD 62

Query: 67  HAAVAAAAKDSAAALGKVDILICSAGIT-GATVPVWEFPVDSFQRVIDINLNGLFYCNRE 125
           +A+ +A A      LG+VDIL+ +AGIT  AT      P   +  V+  NL+ LF  +++
Sbjct: 63  YASCSAMAAALREKLGEVDILVNNAGITRDATFRKLSQP--DWDAVMGTNLDSLFNVSKQ 120

Query: 126 VVPFMLENGYGRIVNLASVAGKEGNPNASAYSASKAGVIGFTKSLGKELAGKGVIANALT 185
            V  M E G+GR++N++S+ G++G    + YSA+KAG+ GFT +L +E+A KGV  N ++
Sbjct: 121 FVDSMSERGWGRVINISSINGQKGQFGQTNYSAAKAGMHGFTMALAQEVARKGVTVNTIS 180

Query: 186 PATFESPILDQLPQSQVDYMRSKIPMGRLGLVEESAAMVCFMASEECSFTTASTFDTSGG 245
           P    + ++  +P    + +  +IP+GRLG  EE A+++ ++ASE   F T +    +GG
Sbjct: 181 PGYIATEMVMAVPVEIRERIVGQIPVGRLGKPEEVASLIVWLASEMAGFVTGANIAINGG 240

Query: 246 RTT 248
           + T
Sbjct: 241 QHT 243


Lambda     K      H
   0.318    0.132    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 164
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 249
Length of database: 244
Length adjustment: 24
Effective length of query: 225
Effective length of database: 220
Effective search space:    49500
Effective search space used:    49500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory