GapMind for catabolism of small carbon sources

 

Protein WP_054255254.1 in Acidovorax caeni R-24608

Annotation: NCBI__GCF_001298675.1:WP_054255254.1

Length: 505 amino acids

Source: GCF_001298675.1 in NCBI

Candidate for 23 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine catabolism iolA hi malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 72% 99% 726.5
myo-inositol catabolism mmsA hi malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 72% 99% 726.5
propionate catabolism iolA hi malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 72% 99% 726.5
L-threonine catabolism iolA hi malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 72% 99% 726.5
L-valine catabolism iolA hi malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 72% 99% 726.5
L-valine catabolism mmsA med methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 72% 100% 733 Putative 3-oxopropanoate dehydrogenase; EC 1.2.1.- 73% 729.2
L-valine catabolism mmsA med mmsA: methylmalonate-semialdehyde dehydrogenase (acylating) (EC 1.2.1.27) (TIGR01722) 100% 667.2 Putative 3-oxopropanoate dehydrogenase; EC 1.2.1.- 73% 729.2
L-arabinose catabolism xacF lo Alpha-ketoglutarate semialdehyde dehydrogenase; KGSADH; EC 1.2.1.26 (characterized) 35% 99% 268.5 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
D-galacturonate catabolism dopDH lo Alpha-ketoglutarate semialdehyde dehydrogenase; KGSADH; EC 1.2.1.26 (characterized) 35% 99% 268.5 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
D-glucuronate catabolism dopDH lo Alpha-ketoglutarate semialdehyde dehydrogenase; KGSADH; EC 1.2.1.26 (characterized) 35% 99% 268.5 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
D-xylose catabolism dopDH lo Alpha-ketoglutarate semialdehyde dehydrogenase; KGSADH; EC 1.2.1.26 (characterized) 35% 99% 268.5 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-phenylalanine catabolism pad-dh lo aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 33% 90% 231.1 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-arginine catabolism gabD lo succinate-semialdehyde dehydrogenase (EC 1.2.1.16) (characterized) 34% 93% 226.1 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-citrulline catabolism gabD lo succinate-semialdehyde dehydrogenase (EC 1.2.1.16) (characterized) 34% 93% 226.1 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
putrescine catabolism gabD lo succinate-semialdehyde dehydrogenase (EC 1.2.1.16) (characterized) 34% 93% 226.1 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-arginine catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 33% 93% 221.5 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-citrulline catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 33% 93% 221.5 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-lysine catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 33% 93% 221.5 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-proline catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 33% 93% 221.5 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-arginine catabolism putA lo L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) 30% 92% 211.8 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-arginine catabolism rocA lo L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) 30% 92% 211.8 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-citrulline catabolism putA lo L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) 30% 92% 211.8 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-citrulline catabolism rocA lo L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) 30% 92% 211.8 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0
L-proline catabolism putA lo L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized) 30% 92% 211.8 methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) 72% 733.0

Sequence Analysis Tools

View WP_054255254.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

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Fitness BLAST: loading...

Sequence

MQNDNNVTQTIGHLIDGQLVADTERTQPVFNPATGQSTTRVALASQATVEAAIASAEAAF
PAWRNTPPLKRARVMSKLKVLLEENADKIAALITAEHGKVLADAHGELQRGIENVEYASY
APELLKGEHSRNVGPSIDSWSEFQALGVTAGITPFNFPAMVPLWMWPMAVACGNTFVLKP
SERDPTSALFIAQLALEAGLPPGVLNVVNGDKLAVDTLLQDPRVKAVSFVGSTPIAEYIY
AEGCKHGKRVQALGGAKNHAVLMPDADVDNAVSALMGAAYGSCGERCMAIPLLVAVGDAV
GDAVIAGLKTEIAKMKVGPGTDNSNDMGPLVTKPHFEKVKAYVDSGVAEGASLVVDGRGV
QVAGHEEGYFLGACLFDHVKPGMKIYQEEIFGPVLGVVRVKTLQEAMQLINDHEYGNGTC
IFTRDGEAARYFTDHIQVGMVGVNVPLPVPVAYHSFGGWKRSLFGDLHAYGPDAVRFYTK
RKTITQRWPSAGVREGAVFSFPSSR

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory