Align ABC transporter for L-Lysine, permease component 2 (characterized)
to candidate WP_054254771.1 BN2503_RS00920 ABC transporter permease subunit
Query= reanno::pseudo5_N2C3_1:AO356_05505 (236 letters) >NCBI__GCF_001298675.1:WP_054254771.1 Length = 237 Score = 178 bits (451), Expect = 9e-50 Identities = 98/221 (44%), Positives = 145/221 (65%), Gaps = 12/221 (5%) Query: 22 GLAMTLWLLSASLLIGFVVSIPLSIARVSPKFYVRWPVQFYTYLFRGTPLYIQLLICYTG 81 GL +TL L SL +G V+++ ++A VSP+ ++RWP Q +TY RGTPL IQ+ + Y G Sbjct: 18 GLVVTLQLTLYSLGLGAVLALACALALVSPRVWLRWPAQTFTYFMRGTPLLIQVYLIYYG 77 Query: 82 IYSIAAVRAQ-----PMLDSFFRDAMNCTILAFALNTCAYTTEIFAGAIRSMNHGEVEAA 136 + + V+A+ P F++ CT+L+F+LNT AYT E AG+IR N GEVEAA Sbjct: 78 VAQLEWVQARWDAVWPWTQ--FKEPFFCTLLSFSLNTAAYTAETLAGSIRETNKGEVEAA 135 Query: 137 KAYGLTGWKLYAYVIMPSALRRSLPYYSNEVILMLHSTTVAFTATVPDVLKVARDAN--- 193 +A G+ L +++PSA+RR LP Y NEV++MLHS+++A +TVP +L + A+ Sbjct: 136 RAMGMGHGMLMRRIVLPSAIRRMLPAYGNEVVMMLHSSSLA--STVPALLDLTGAASRVY 193 Query: 194 SATFLTFQSFGIAALIYLTVTFALVGLFRLAERRWLAFLGP 234 + FL F+++ AA IYL +TFAL+G +L ERR+L +L P Sbjct: 194 ADFFLPFEAYLFAAAIYLCITFALIGASKLLERRFLGYLAP 234 Lambda K H 0.329 0.140 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 181 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 236 Length of database: 237 Length adjustment: 23 Effective length of query: 213 Effective length of database: 214 Effective search space: 45582 Effective search space used: 45582 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory