GapMind for catabolism of small carbon sources

 

Protein WP_143773519.1 in Neptunomonas antarctica S3-22

Annotation: NCBI__GCF_001305295.1:WP_143773519.1

Length: 487 amino acids

Source: GCF_001305295.1 in NCBI

Candidate for 24 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism praB med 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 46% 98% 439.5 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-tryptophan catabolism praB med 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 46% 98% 439.5 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-tryptophan catabolism nbaE med aminomuconate-semialdehyde dehydrogenase (EC 1.2.1.32) (characterized) 43% 95% 386 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-phenylalanine catabolism pad-dh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 41% 91% 359.8 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-arginine catabolism kauB med 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 40% 96% 342.4 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-arginine catabolism puuC med 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 40% 96% 342.4 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-citrulline catabolism puuC med 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 40% 96% 342.4 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
putrescine catabolism puuC med 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) 40% 96% 342.4 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-arginine catabolism patD lo 4-trimethylammoniobutyraldehyde dehydrogenase (EC 1.2.1.47) (characterized) 37% 97% 338.2 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-citrulline catabolism patD lo 4-trimethylammoniobutyraldehyde dehydrogenase (EC 1.2.1.47) (characterized) 37% 97% 338.2 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
putrescine catabolism patD lo 4-trimethylammoniobutyraldehyde dehydrogenase (EC 1.2.1.47) (characterized) 37% 97% 338.2 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-arginine catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 38% 97% 331.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-citrulline catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 38% 97% 331.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-fucose catabolism aldA lo NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 38% 95% 331.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-lysine catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 38% 97% 331.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-proline catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 38% 97% 331.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-rhamnose catabolism aldA lo NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 38% 95% 331.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-threonine catabolism aldA lo NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 38% 95% 331.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-arabinose catabolism xacF lo Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 39% 98% 311.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
D-galacturonate catabolism dopDH lo Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 39% 98% 311.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
D-glucuronate catabolism dopDH lo Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 39% 98% 311.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
D-xylose catabolism dopDH lo Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 39% 98% 311.6 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-lysine catabolism patD lo aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) 38% 99% 310.1 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7
L-valine catabolism mmsA lo Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (characterized) 33% 93% 245 subunit of 5-carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenase (EC 1.2.1.60) 69% 705.7

Sequence Analysis Tools

View WP_143773519.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MIKNLINGKWVESKETFETLNPATGEVLAEVAHASDAQVAEAVAAAKAAFPAWANMPVAK
RSKIITNLGDLIAANVEELAQMETADTGLPLYQTQNALIPRASNNFYFFAEMAKQMSGHT
YPMDDQMLNYSLYQPTGVCALISPWNVPFMTGTWKLAPCLALGNTAVMKQSELSPLTTDF
LGRLALEAGVPAGVFNIVHGFGATTGNALITNPDVAAISFTGGTSTGKHIIATAGLKKFS
MELGGKSPVMIFDDCDYERALDAAIFGIFSINGERCTAGSRIFVQETIYDRFCKDFAERT
NKIEVGDPTDINTKVGSLISQSHWEKVTGYIQTGIEEGATLIAGGPEKPTAELPDHLKGG
HFIRPTAFRDVTNDMRIAREEIFGPVAVLIPFKDEEDVIRMANDNDYGLASYLWTQDNGK
VHRIAHRIEAGMLFVNSQNVRDLRTPFGGMKASGTGREGGTYSYEIFCEPKNVCISMGSH
HIPKWGV

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory