Alignments for a candidate for acnD in Neptunomonas antarctica S3-22

GapMind for catabolism of small carbon sources

Alignments for a candidate for acnD in Neptunomonas antarctica S3-22

Align 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (EC 4.2.1.117) (characterized)
to candidate WP_054342411.1 Nant_RS14685 Fe/S-dependent 2-methylisocitrate dehydratase AcnD

Query= BRENDA::Q8EJW3
         (867 letters)



>NCBI__GCF_001305295.1:WP_054342411.1
          Length = 869

 Score = 1402 bits (3629), Expect = 0.0
 Identities = 701/869 (80%), Positives = 761/869 (87%), Gaps = 9/869 (1%)

Query: 5   MNTQYRKPLPGTALDYFDTREAIEAIAPGAYAKLPYTSRVLAENLVRRCEPEMLTASLKQ 64
           MNT+YRK LPGT LDYFDTR AIE I PG+YAKLPYTSRVLAE LVRRCEPE LT SLKQ
Sbjct: 1   MNTEYRKSLPGTKLDYFDTRAAIEDIQPGSYAKLPYTSRVLAEQLVRRCEPEALTDSLKQ 60

Query: 65  IIESKQELDFPWFPARVVCHDILGQTALVDLAGLRDAIAAKGGDPAQVNPVVPTQLIVDH 124
           +IE K+ELDFPW+PARVVCHDILGQTALVDLAGLRDAIA +GGDP++VNPVVPTQLIVDH
Sbjct: 61  LIEFKRELDFPWYPARVVCHDILGQTALVDLAGLRDAIAEQGGDPSKVNPVVPTQLIVDH 120

Query: 125 SLAVEYGGFDKDAFAKNRAIEDRRNEDRFHFINWTQKAFKNIDVIPQGNGIMHQINLERM 184
           SLAVE  GFD DAF KNRAIEDRRN+DRFHFI W + AF N+DVIP GNGIMHQINLE+M
Sbjct: 121 SLAVEAAGFDPDAFEKNRAIEDRRNDDRFHFIEWCKTAFDNVDVIPAGNGIMHQINLEKM 180

Query: 185 SPVIHARNGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAESVMLGRASYMRLPDIIGV 244
           SPVI AR+GVA+PDT VGTDSHTPHVDALGV+AIGVGGLEAE+VMLG  S MRLPDIIGV
Sbjct: 181 SPVIQARDGVAYPDTCVGTDSHTPHVDALGVLAIGVGGLEAETVMLGLPSMMRLPDIIGV 240

Query: 245 ELTGKPQPGITATDIVLALTEFLRAQKVVSSYLEFFGEGAEALTLGDRATISNMTPEFGA 304
           +LTGK QPGITATDIVLA+TEFLR QKVVS+YLEFFGEGA+ LT+GDRATISNMTPEFGA
Sbjct: 241 KLTGKRQPGITATDIVLAITEFLRNQKVVSAYLEFFGEGAKNLTIGDRATISNMTPEFGA 300

Query: 305 TAAMFYIDQQTLDYLTLTGREAEQVKLVETYAKTAGLWSDDLKQAVYPRTLHFDLSSVVR 364
           +A MFYID+QT+ YL LTGRE EQV LVE YAK  GLW+DDL+   Y R L FDLSSVVR
Sbjct: 301 SAGMFYIDEQTIAYLKLTGREPEQVALVEQYAKLTGLWADDLENVEYERVLEFDLSSVVR 360

Query: 365 TIAGPSNPHARVPTSELAARGISGE-------VENEPGLMPDGAVIIAAITSCTNTSNPR 417
            +AGPSNPH R+PTS L  RGI+ E        E E GLMPDGAVIIAAITSCTNTSNPR
Sbjct: 361 NMAGPSNPHRRLPTSALKERGIASEEMLAAAKAEEEAGLMPDGAVIIAAITSCTNTSNPR 420

Query: 418 NVIAAGLLARNANAKGLTRKPWVKTSLAPGSKAVQLYLEEANLLPELESLGFGIVGFACT 477
           NV+AAGL+A+ AN  GL RKPWVKTS APGSK  +LYLEEA LL E+E LGFGIVG+ACT
Sbjct: 421 NVVAAGLVAKKANELGLVRKPWVKTSFAPGSKVAKLYLEEAGLLSEMEQLGFGIVGYACT 480

Query: 478 TCNGMSGALDPVIQQEVIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAG 537
           TCNGMSGALDPVIQQE+IDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAG
Sbjct: 481 TCNGMSGALDPVIQQEIIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAG 540

Query: 538 TIRFDIEKDVLGLDKDGKPVRLINIWPSDAEIDAVIAASVKPEQFRKVYEPMFDLSVDYG 597
           TIRFDIEKDVLG DKDG P+ L +IWPSD EID+++A +VKPEQF ++Y PMFDL     
Sbjct: 541 TIRFDIEKDVLGTDKDGNPITLKDIWPSDEEIDSIVAKAVKPEQFSQIYIPMFDLGT-VE 599

Query: 598 DKVSPLYDWRPQSTYIRRPPYWEGALAGERTLKGMRPLAVLGDNITTDHLSPSNAIMMDS 657
             VSPLYDWRPQ+TYIRRPPYWEGALAGERT+KGMRPLAVLGDNITTDHLSPSNAI   S
Sbjct: 600 KAVSPLYDWRPQTTYIRRPPYWEGALAGERTMKGMRPLAVLGDNITTDHLSPSNAIQASS 659

Query: 658 AAGEYLHKMGLPEEDFNSYATHRGDHLTAQRATFANPKLKNEMAIVD-GKVKQGSLARIE 716
           AAGEY  KMGLPEEDFNSYATHRGDHLTAQRATFANPKL NEM + + G+VKQGSLARIE
Sbjct: 660 AAGEYCAKMGLPEEDFNSYATHRGDHLTAQRATFANPKLLNEMCLDEKGEVKQGSLARIE 719

Query: 717 PEGIVTRMWEAIETYMDRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIH 776
           PEG  +RMWEAIETYM+RKQPLII+AGADYGQGSSRDWAAKGVRLAGVEAI+AEGFERIH
Sbjct: 720 PEGKESRMWEAIETYMERKQPLIIVAGADYGQGSSRDWAAKGVRLAGVEAIMAEGFERIH 779

Query: 777 RTNLVGMGVLPLEFKAGENRATYGIDGTEVFDVIGSIAPRADLTVIITRKNGERVEVPVT 836
           RTNLVGMGVLPL+FK GE R TY IDGTE FDV G I+PRA+L VII R NGE V+VP+ 
Sbjct: 780 RTNLVGMGVLPLQFKEGETRKTYAIDGTETFDVEGDISPRAELNVIINRANGETVKVPMI 839

Query: 837 CRLDTAEEVSIYEAGGVLQRFAQDFLESN 865
           CRLDTA EV++Y AGGVLQRFAQDFLE N
Sbjct: 840 CRLDTAAEVNVYNAGGVLQRFAQDFLEGN 868


Lambda     K      H
   0.318    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2183
Number of extensions: 89
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 867
Length of database: 869
Length adjustment: 42
Effective length of query: 825
Effective length of database: 827
Effective search space:   682275
Effective search space used:   682275
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources