Align Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate WP_045582841.1 AL072_RS18480 amino acid ABC transporter ATP-binding protein
Query= TCDB::Q9HU32 (257 letters) >NCBI__GCF_001305595.1:WP_045582841.1 Length = 251 Score = 242 bits (618), Expect = 5e-69 Identities = 129/251 (51%), Positives = 170/251 (67%), Gaps = 9/251 (3%) Query: 3 EATPALEIRNLHKRYGDLEVLKGISLTARDGDVISILGSSGSGKSTFLRCINLLENPHQG 62 ++ P +E+R+++K +G VLKG++L+ RDG V+ I+G SGSGKST LRCIN L +G Sbjct: 6 QSAPFIEMRDVYKSFGSTAVLKGVTLSVRDGGVVCIIGPSGSGKSTILRCINGLMTIDRG 65 Query: 63 QILVSGEELRLKKSKNGDLVAADSQQINRLRSELGFVFQNFNLWPHMSILDNVIEAPRRV 122 I V + KS +Q+ LR ++ VFQ +NL+PH ++L N+ AP +V Sbjct: 66 TIRVGSFAVETLKS---------DKQLVPLRHQVAMVFQQYNLFPHRTVLQNLTMAPVQV 116 Query: 123 LGKSKAEAIEIAEGLLAKVGIADKRHSYPAQLSGGQQQRAAIARTLAMQPKVILFDEPTS 182 LG++ A E A LLAKV + K+ SYP +LSGGQQQR AIAR LAMQPKVILFDE T+ Sbjct: 117 LGENPAAVRERALALLAKVRLTGKQDSYPGELSGGQQQRVAIARALAMQPKVILFDEVTA 176 Query: 183 ALDPEMVQEVLNVIRALAEEGRTMLLVTHEMSFARQVSSEVVFLHQGLVEEQGTPQQVFE 242 ALDPEMV EVLNVIR LA EG T +LVTHEM FA++V+ EV F +G++ E G P +F+ Sbjct: 177 ALDPEMVSEVLNVIRDLAREGMTCVLVTHEMRFAQEVADEVFFTDRGVIVEHGPPGTIFQ 236 Query: 243 NPQSARCKQFM 253 NP R K F+ Sbjct: 237 NPTDPRLKAFL 247 Lambda K H 0.317 0.133 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 165 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 257 Length of database: 251 Length adjustment: 24 Effective length of query: 233 Effective length of database: 227 Effective search space: 52891 Effective search space used: 52891 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory