Align propionyl-CoA carboxylase α subunit (EC 6.4.1.3) (characterized)
to candidate WP_045586135.1 AL072_RS14875 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha
Query= metacyc::MONOMER-13589 (666 letters) >NCBI__GCF_001305595.1:WP_045586135.1 Length = 666 Score = 827 bits (2135), Expect = 0.0 Identities = 425/670 (63%), Positives = 513/670 (76%), Gaps = 10/670 (1%) Query: 1 MFKKILIANRGEIACRVIKTARKMGIQTVAVYSDADRNALHVSMADEAIHIGPPPANQSY 60 MF+KILIANRGEIACRVI+TAR+MGI+TVAVYSDAD+NALHV MADEA+HIGPP + QSY Sbjct: 1 MFEKILIANRGEIACRVIRTARRMGIKTVAVYSDADKNALHVEMADEAVHIGPPASAQSY 60 Query: 61 IVIDKIMEAIKASGAEAVHPGYGFLSERMDFAAALEAAGVVFIGPPSGAIEAMGDKITSK 120 ++ID+I++A K +GA+AVHPGYGFLSE+ F AL AAG+ FIGP + A+ AMGDKI SK Sbjct: 61 LLIDRIVDACKRTGAQAVHPGYGFLSEKPGFQEALAAAGIAFIGPDAHAMRAMGDKIESK 120 Query: 121 KLAKEAGVSTVPGYMGLIADADEAVKISNEIGYPVMIKASAGGGGKGMRIAWSEAEVKEG 180 KLAK AGVSTVPGY+G+IAD +EAV I+ +IGYPVMIKASAGGGGKGMR+AW++ E +EG Sbjct: 121 KLAKAAGVSTVPGYLGVIADDEEAVMIARDIGYPVMIKASAGGGGKGMRVAWNDEEAREG 180 Query: 181 FESSKNEAANSFGDDRIFIEKFVTQPRHIEIQVLADKHGNCVYLHERECSIQRRNQKVIE 240 F S+ NEA +SF DDR+F+EK++ QPRHIEIQ+LAD G C+YLHERECSIQRR+QKVIE Sbjct: 181 FRSATNEARSSFADDRVFVEKYIQQPRHIEIQLLADGQGTCLYLHERECSIQRRHQKVIE 240 Query: 241 EAPSPFLDEATRKAMGEQACALAKAVGYASAGTVEFIVDGQKNFYFLEMNTRLQVEHPVT 300 EAPSPFLD ATRKAMGEQA ALA AV Y SAGTVEFIVD ++NFYFLEMNTRLQVEHPVT Sbjct: 241 EAPSPFLDAATRKAMGEQAVALANAVQYKSAGTVEFIVDAERNFYFLEMNTRLQVEHPVT 300 Query: 301 ELITGIDLVEQMIRVAAGEKLPFQQSDLKINGWAMESRLYAEDPYRNFLPSIGRLTRYRP 360 ELITG+DLVE MIRVAAGEKL Q D+ +NGWA+E+R+YAEDP+RNFLPS GRLT YRP Sbjct: 301 ELITGLDLVELMIRVAAGEKLTIGQDDVTLNGWAIEARVYAEDPFRNFLPSTGRLTNYRP 360 Query: 361 PVESVTPTSVVRNDTGVYEGGEISMYYDPMIAKLCTWAPTREAAIEEMRLALDTFEVEGI 420 P VR DTGV+EGGEISMYYDPMIAKLCTW TR+AAI MR ALD + + G+ Sbjct: 361 PAAD----PHVRVDTGVFEGGEISMYYDPMIAKLCTWGSTRDAAIGGMREALDQYYIRGV 416 Query: 421 GHNLPFVGAVMDHPRFVKGDITTAFIAEEYPDGFQGAVLDEPTLRRVAAAAAAMNRVAEI 480 HN+PF+ ++M + RFV G +TT FIAEEYP+GF + L + A AA ++R Sbjct: 417 SHNIPFLASLMANQRFVDGRLTTNFIAEEYPNGFHASDLPPEDPAVLVAVAAVIHRSLND 476 Query: 481 RRTRISGTMNNHERHVGVDWVVALQGESYHVSIAA-----DREGSTVSFSDGSSLRVTSD 535 R +ISG M + V DWVV L G ++ V + A R G TV + G V SD Sbjct: 477 RDIQISGKMAGSKVTVRDDWVVVLDGTAHPVHLHAAAGSPQRIGYTVDIA-GRHHTVWSD 535 Query: 536 WTPGQPLASLMVDGRPLVMKVGKIPMGFRLRLRGADLKVNVRTPRQAELALLMPEKLPPD 595 W G+PL VDG + +++ ++ +G+RL G+ V V TP+ A L LMP K PPD Sbjct: 536 WQIGEPLFRGTVDGSHVCVQIDRVGIGYRLHHSGSQAIVKVLTPKAAALDALMPVKAPPD 595 Query: 596 TSKYLLCPMPGLVVKINVAEGDEVQEGQALATVEAMKMENILRAERRGTVKKIAAAPGAS 655 SK+LL PMPGL+V + V+EG E++ G+ LA VEAMKMENILRA + GTV KI AAPG+S Sbjct: 596 MSKFLLSPMPGLLVSLAVSEGQEIKAGEVLAVVEAMKMENILRASQDGTVAKIHAAPGSS 655 Query: 656 LRVDDVIMEF 665 L VD I+EF Sbjct: 656 LAVDQKIVEF 665 Lambda K H 0.318 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1205 Number of extensions: 44 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 666 Length of database: 666 Length adjustment: 38 Effective length of query: 628 Effective length of database: 628 Effective search space: 394384 Effective search space used: 394384 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory