Align AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized)
to candidate WP_054557498.1 I595_RS00860 ABC transporter ATP-binding protein
Query= TCDB::Q52815 (257 letters) >NCBI__GCF_001306415.1:WP_054557498.1 Length = 219 Score = 132 bits (331), Expect = 8e-36 Identities = 80/220 (36%), Positives = 128/220 (58%), Gaps = 10/220 (4%) Query: 18 VEIVNMNKWYGDFHVLRDINLKVMRGERIVIAGPSGSGKSTMIRCINRLEEHQKGK---I 74 + N+ K YGD VL+ +++ + +GE I I GPSG+GK+T+++ + L+ K I Sbjct: 2 ITATNIKKNYGDLKVLKGVSISIKKGEVISIVGPSGAGKTTLLQLVGTLDTPSNPKDSSI 61 Query: 75 VVDGTELTNDL--KKIDEVRRE-VGMVFQHFNLFPHLTILENCTLAPIWVRKMPKKQAEE 131 +++G ++T DL K++ + R E +G +FQ L P T LEN + P +++K PK +AE Sbjct: 62 MINGVDVT-DLRDKQLAKFRNENIGFIFQFHQLLPEFTALENVCI-PAFIKKTPKAKAEV 119 Query: 132 VAMHFLKRVKIPEQANKYPGQLSGGQQQRVAIARSLCMNPKIMLFDEPTSALDPEMIKEV 191 L + + ++ + P +LSGG+QQRVA+AR+L NP ++ DEP+ LD E + + Sbjct: 120 RGRELLDFLGLSQRYDHRPRELSGGEQQRVAVARALINNPSVIFADEPSGNLDSESAENL 179 Query: 192 LDTMVGLAEE-GMTMLCVTHEMGFARQVANRVIFMDQGQI 230 L EE G T + VTH A +A+R + M GQI Sbjct: 180 HQLFFKLREEFGQTFVIVTHNKELA-NMADRKLIMVDGQI 218 Lambda K H 0.321 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 135 Number of extensions: 6 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 257 Length of database: 219 Length adjustment: 23 Effective length of query: 234 Effective length of database: 196 Effective search space: 45864 Effective search space used: 45864 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory