Align Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized)
to candidate WP_054559826.1 I595_RS12740 ABC transporter ATP-binding protein
Query= TCDB::Q72L52 (376 letters) >NCBI__GCF_001306415.1:WP_054559826.1 Length = 325 Score = 131 bits (329), Expect = 3e-35 Identities = 73/221 (33%), Positives = 121/221 (54%), Gaps = 6/221 (2%) Query: 19 VKDFNLETEDGEFVVFVGPSGCGKTTTLRMIAGLEEISEGNIYIGDRLV----NDVPPKD 74 +KD + E G + +G SG GK+T L+ I GL E+ +G ++ G V ++ P + Sbjct: 17 LKDVSFSIEKGTHLSIMGESGSGKSTLLKAIYGLLELEKGTVFWGKEQVLGPNYNLVPGE 76 Query: 75 RDIAMVFQNYALYPHMNVYENMAFGLRLRRYPKDEIDRRVKEAARILKIEHLLNRKPREL 134 + + V Q++ L P V EN+A L + D R+ E I+++E + K + L Sbjct: 77 KYMKYVAQDFDLMPFTTVTENIAE--HLSAFEMDSHQARITELLHIIEMEEFAHVKVKNL 134 Query: 135 SGGQRQRVAMGRAIVREPKVFLMDEPLSNLDAKLRVEMRAEIAKLQRRLGVTTIYVTHDQ 194 SGGQ+QRVA+ RA+ +EP+V L+DEP S++D + E+R ++ R G+T I +HD Sbjct: 135 SGGQQQRVALARALAQEPEVLLLDEPFSSIDQFKKNELRYKLFPYLREKGITVINASHDP 194 Query: 195 VEAMTLGHRIVVMKDGEIQQVDTPLNLYDFPANRFVAGFIG 235 + + +V+K+GEI + + LY P ++VA G Sbjct: 195 NDVLAFADETIVLKNGEILAHEPTVQLYQIPKEKYVASLFG 235 Lambda K H 0.320 0.139 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 246 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 376 Length of database: 325 Length adjustment: 29 Effective length of query: 347 Effective length of database: 296 Effective search space: 102712 Effective search space used: 102712 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory