Protein WP_055435026.1 in Lacinutrix algicola AKS293
Annotation: NCBI__GCF_001418085.1:WP_055435026.1
Length: 404 amino acids
Source: GCF_001418085.1 in NCBI
Candidate for 22 steps in catabolism of small carbon sources
Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
4-hydroxybenzoate catabolism | paaJ2 | hi | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 (characterized) | 58% | 100% | 463.8 | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] | 47% | 333.2 |
4-hydroxybenzoate catabolism | paaJ2 | med | pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (TIGR02430) | | 100% | 602 | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] | 47% | 333.2 |
phenylacetate catabolism | paaJ2 | hi | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 (characterized) | 58% | 100% | 463.8 | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] | 47% | 333.2 |
phenylacetate catabolism | paaJ2 | med | pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (TIGR02430) | | 100% | 602 | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] | 47% | 333.2 |
L-phenylalanine catabolism | paaJ2 | hi | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 (characterized) | 58% | 100% | 463.8 | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] | 47% | 333.2 |
L-phenylalanine catabolism | paaJ2 | med | pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (TIGR02430) | | 100% | 602 | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] | 47% | 333.2 |
phenylacetate catabolism | paaJ1 | hi | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 (characterized) | 58% | 100% | 463.8 | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] | 47% | 333.2 |
L-phenylalanine catabolism | paaJ1 | hi | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 (characterized) | 58% | 100% | 463.8 | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] | 47% | 333.2 |
4-hydroxybenzoate catabolism | pcaF | med | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 (characterized) | 58% | 100% | 463.8 | Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) | 57% | 454.9 |
4-hydroxybenzoate catabolism | pcaF | med | pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (TIGR02430) | | 100% | 602 | Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) | 57% | 454.9 |
L-tryptophan catabolism | pcaF | med | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 (characterized) | 58% | 100% | 463.8 | Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) | 57% | 454.9 |
L-tryptophan catabolism | pcaF | med | pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (TIGR02430) | | 100% | 602 | Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) | 57% | 454.9 |
L-isoleucine catabolism | fadA | med | subunit of β-ketoadipyl CoA thiolase (EC 2.3.1.174; EC 2.3.1.16) (characterized) | 52% | 100% | 416.8 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
L-isoleucine catabolism | fadA | med | acetyl-CoA C-acyltransferase (EC 2.3.1.16) (TIGR01930) | | 100% | 459.4 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
4-hydroxybenzoate catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
L-arginine catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
L-citrulline catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
2-deoxy-D-ribonate catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
2-deoxy-D-ribose catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
L-leucine catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
L-lysine catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
phenylacetate catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
L-phenylalanine catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
L-proline catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
L-tyrosine catabolism | atoB | med | acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (characterized) | 47% | 100% | 333.2 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
4-hydroxybenzoate catabolism | pimB | lo | 3-oxopimeloyl-CoA:CoA acetyltransferase (characterized) | 40% | 100% | 254.6 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
phenylacetate catabolism | pimB | lo | 3-oxopimeloyl-CoA:CoA acetyltransferase (characterized) | 40% | 100% | 254.6 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
L-phenylalanine catabolism | pimB | lo | 3-oxopimeloyl-CoA:CoA acetyltransferase (characterized) | 40% | 100% | 254.6 | 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 | 58% | 463.8 |
Sequence Analysis Tools
View WP_055435026.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MKEAYIIDGIRTPIGNYKGTLSAVRTDDLGALVISEIVKRNPSIPKEAYDDVIMGCANQA
GEDNRNVARMSSLLAGLPFTVPGETVNRLCSSGLSAIIHANRAIKAGDGDVFISGGVENM
TRGPYVMAKPSTAFGGDSKMYDSTFGWRFINPKMQALYGTDGMGNTAENLVEKYNISRED
QDKFALWSQQKATKAQESGRLAKEIVTVEIPQRKKDPIQFSKDEFIKPTSSLEVLGKLRA
AFKKEGGSVTAGNSSGLNDGAAATIIASADAVKKYNLKPLARIVSSAVVGVEPRIMGIGP
VQASNKALAKAGLTMDDIDIIELNEAFAAQALACIRAWGIADNDPRINPNGGAIAIGHPL
GVTGARVAHSAALELQNQNKKYALITMCIGVGQGYAAIIENVNI
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory