GapMind for catabolism of small carbon sources

 

Alignments for a candidate for bgtA in Lacinutrix algicola AKS293

Align ATPase (characterized, see rationale)
to candidate WP_055435910.1 ASC41_RS06935 ABC transporter ATP-binding protein

Query= uniprot:Q31RN8
         (261 letters)



>NCBI__GCF_001418085.1:WP_055435910.1
          Length = 233

 Score =  132 bits (332), Expect = 6e-36
 Identities = 79/208 (37%), Positives = 118/208 (56%), Gaps = 4/208 (1%)

Query: 33  NQFQALCGVSLTVQRGEVVVMMGPSGSGKSTFLRTLNALESHQRGEIWIEGHRLSHDRRD 92
           +    L G+ L+V+ GE+V +MG SGSGKST L  +  L+    GE  ++   + +    
Sbjct: 16  SSLHVLKGIDLSVEAGEMVAIMGSSGSGKSTLLNIIGMLDEADEGEYILDNVPIKNLTEK 75

Query: 93  IATIRQE--VGMVFQQFNLFPHLTVLQNLMLAPVQVRRWPVAQAEATARQLLERVRIAEQ 150
            A I +   +G +FQ FNL  +   L N+ L P+  +     +        LE+V + + 
Sbjct: 76  KAAIYRNKFLGFIFQSFNLINYKNALDNVAL-PLYYQGLKRKERIEKGLFHLEKVGLKDW 134

Query: 151 ADKYPGQLSGGQQQRVAIARALAMQPRILLFDEPTSALDPEMVREVLDVMRDLASEGMTM 210
           A   P +LSGGQ+QRVAIARALA  P++LL DEPT ALD +   E++D ++ L  EG T+
Sbjct: 135 AHHLPNELSGGQKQRVAIARALAADPKLLLADEPTGALDTKTSHEIMDFIQSLNDEGKTI 194

Query: 211 LVATHEVGFAREVADRVVLMADGQIVEE 238
           L+ THE   A  +  R+V + DG I+E+
Sbjct: 195 LMVTHEEDIA-SMCKRIVRLKDGVIIED 221


Lambda     K      H
   0.321    0.134    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 128
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 261
Length of database: 233
Length adjustment: 24
Effective length of query: 237
Effective length of database: 209
Effective search space:    49533
Effective search space used:    49533
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory