Alignments for a candidate for acs in Kocuria flava HO-9041

GapMind for catabolism of small carbon sources

Alignments for a candidate for acs in Kocuria flava HO-9041

Align Acetate/butyrate--CoA ligase AAE7, peroxisomal; AMP-binding protein 7; AtAMPBP7; Acetyl-CoA synthetase; Acyl-activating enzyme 7; Butyryl-CoA synthetase; Protein ACETATE NON-UTILIZING 1; EC 6.2.1.1; EC 6.2.1.2 (characterized)
to candidate WP_058857969.1 AS188_RS05250 AMP-binding protein

Query= SwissProt::Q8VZF1
         (569 letters)



>NCBI__GCF_001482365.1:WP_058857969.1
          Length = 525

 Score =  438 bits (1126), Expect = e-127
 Identities = 234/542 (43%), Positives = 322/542 (59%), Gaps = 24/542 (4%)

Query: 17  ANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRSIGPGSTVA 76
           AN+  L+PL FL R+A V P R++V+HG+R +T+R+     +RLA AL    + PG  VA
Sbjct: 3   ANHEPLSPLRFLQRSAEVFPDREAVVHGTRRWTYRRFAAEAQRLARALGPH-LEPGGRVA 61

Query: 77  IIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQEFFTLAEDS 136
           ++APN+P M  AH+ VP+ G VL  +N RL+   + ++L HS++ V++ D E        
Sbjct: 62  VVAPNVPEMLLAHYAVPLAGGVLVPLNPRLSGRELGYVLGHSEARVVLADTEVVATVARV 121

Query: 137 LRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGD--PNYPWQPPA 194
            R +E         P+++ + D T        A  +GA+  E FL  G   P+ PW+  A
Sbjct: 122 ARQLEPA-------PVVVEVNDPTTGAPP---AAPEGAVALERFLEAGPDGPDLPWRV-A 170

Query: 195 DEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPMFHCNGWC 254
           DE   I L YTSGTT  PKGV+  HRGAY+ AL      G      YLWTLPMFHCNGWC
Sbjct: 171 DENTPITLNYTSGTTGPPKGVLYSHRGAYLNALGEVFHNGFDGATRYLWTLPMFHCNGWC 230

Query: 255 FPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDTILPLPHT 314
             W++    G  +CLR V A+ V+       +TH C APVV + IV+A +     PL   
Sbjct: 231 TTWAVTAAGGRHVCLRAVRAEAVWEAFDTEGITHLCGAPVVCSTIVDAEQAH---PLDRP 287

Query: 315 VHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPETQAKLNA 374
           + + TAGA P PSV+  + + G  V H YGL+E YGP T+C  + EW  LPP  +A+  A
Sbjct: 288 LRITTAGAPPAPSVIQRLEEHGMSVVHVYGLTEVYGPHTICEPQDEWAQLPPAERARTMA 347

Query: 375 RQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKETFAGGWF 434
           RQGV     E   V+D +  + VPADG+T GE+V RGN VM GY K+ +A  E F GGW+
Sbjct: 348 RQGVGMLQAESARVVDERM-RDVPADGETLGEVVLRGNNVMLGYFKDEQATAEAFRGGWY 406

Query: 435 HSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVARPDERWQ 494
           H+GD+ V HPD Y+++KDR+KD+IISGGENISS+EVE  +  HP VL+ +VV  PDE+W 
Sbjct: 407 HTGDLGVMHPDGYLQLKDRAKDIIISGGENISSIEVEQALASHPDVLDVAVVGVPDEKWG 466

Query: 495 ESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVF-GPLPKTATGKIQKHI 553
           E P A+V           +  A+++     E+L  + VP S+ F   LP+TATGK++K+ 
Sbjct: 467 ERPVAWVV-----RTGGSSATAEELRAHAAERLARFKVPDSIRFPEQLPRTATGKVRKNE 521

Query: 554 LR 555
           LR
Sbjct: 522 LR 523


Lambda     K      H
   0.319    0.134    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 885
Number of extensions: 49
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 569
Length of database: 525
Length adjustment: 36
Effective length of query: 533
Effective length of database: 489
Effective search space:   260637
Effective search space used:   260637
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources