Alignments for a candidate for paaH in Kocuria flava HO-9041

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaH in Kocuria flava HO-9041

Align Enoyl-CoA hydratase (EC 4.2.1.17); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) (characterized)
to candidate WP_058857967.1 AS188_RS05240 fatty oxidation complex subunit alpha

Query= reanno::SB2B:6935814
         (716 letters)



>NCBI__GCF_001482365.1:WP_058857967.1
          Length = 707

 Score =  298 bits (764), Expect = 5e-85
 Identities = 226/687 (32%), Positives = 334/687 (48%), Gaps = 39/687 (5%)

Query: 17  IARLCFNAPGSVNKFDRETLASLNAALDVLKDSDAKAAVLTSGKDTFIVGADITEFLALF 76
           + RL    PG +  +    LA L   L  L     +A V+T  + +F  GA++ E  +  
Sbjct: 32  LVRLDATEPGGLIIWGSRALADLRDVLAGLDTGAVEAIVVTGNERSFGAGANLKEIRS-- 89

Query: 77  AEEDAKLMEWIAQANVVFNKLEDLPFPTVSAIKGFALGGGCEAILATDFRVADTSA-KIG 135
           A+      E++   +  F  L D P PT S + G ALGGG E  L TD R+    A  +G
Sbjct: 90  AQLRGASEEYVGLGHEAFGLLADAPVPTFSLVTGQALGGGLELALHTDHRIGHRGAGPLG 149

Query: 136 LPETKLGLIPGFGGTVRLPRLIGADNALEWIT----TGKDQRPEDALKVGAIDAVV--AP 189
           LPE +LG  PG+GG   LP LIG   ALE I      G+  R  +AL++G +DAV+  AP
Sbjct: 150 LPECRLGFFPGWGGVHLLPHLIGPAAALEQIVFAPMRGRTLRAPEALELGLLDAVLDAAP 209

Query: 190 --ENLEAAAIQMLNDALAGKLDWQARRARKQAPLTLPKLEAMMSFTTAKGMVYAVAGKHY 247
             E  E+   Q +   L   +D  A R     P      E   +   A+           
Sbjct: 210 GTEQWESGWQQWVATRLEA-IDAGAPRRPAGTPGEADAQEWEQAVEAARARAERTWHGAA 268

Query: 248 PAPMAAVSVVEQAAGMSRAEALVVEHNAFIKLAKTDVATALIGIFLNDQLVKGKAKKASK 307
           PAP+ A+ +V  A   SR E       AF +L   DVA A +  F   +LV  ++++A+ 
Sbjct: 269 PAPLVAIDLVAAARTQSRTENGDRAVRAFAELVVGDVAKASLYAF---ELVSARSRRAAD 325

Query: 308 LAK----DIKHAAVLGAGIMGGGIAYQSAS-KGTPIVMKDINQAALDLGVNEAAKLLSAQ 362
           +       ++ AAV+GAG+M G +A   A   G P+VM D++   L  GV    +  +AQ
Sbjct: 326 VPPVPPAPLRRAAVIGAGLMAGQLASLIAGGAGIPVVMTDLDDERLAAGVARTRERFAAQ 385

Query: 363 VARGRSTPDKMAKVLNNITPALDYAPLKDVNVVVEAVVENPKVKAMVLADVENVVADDAI 422
            A+GR +P++  ++   +T A     L D + V+EAV E+ +VK  VLA  E V+  DA+
Sbjct: 386 AAKGRLSPERAEQLSALVTGARGPEDLADADFVIEAVFEDLEVKRAVLAQWEGVLRPDAV 445

Query: 423 IASNTSTISIDLLAKSLKNPARFCGMHFFNPVHKMPLVEVIRGKDTSEETVASVVAYASK 482
           +A+NTS++S+  +A+ L++P R  G H FNPV   PL+EV+ G+ T +  +A+    A++
Sbjct: 446 LATNTSSLSVTAMAEGLRHPQRVVGFHVFNPVEVTPLLEVVAGERTGDTALATAFDLAAR 505

Query: 483 MGKTPIVVNDCPGFFVNRVLFPYFAGFNGLLADGGDFAAIDKVMEKQFGWPMGPAYLLDV 542
           + +T + V D PGF VNRVL   F      +  G D A  D+  +   G PM P  LLD 
Sbjct: 506 LRRTAVRVQDAPGFVVNRVLTRMFDVVVRAVDAGTDPAVADRAPD-PMGLPMTPLRLLDF 564

Query: 543 VGLDTGHHAQAVMADGFPDRMGKSDKDAIDVMYEAGRLGQKNGKGFYQYSIDKRGKPKKD 602
           VG    HH  A M + +P+R  +S    +D + EAG           Q+ +  R +P   
Sbjct: 565 VGPAVLHHVNATMHEAYPERFARS--PWMDAVVEAG----------LQHVLPTREEPATG 612

Query: 603 VDPASYTMLAEAFGAQK------AFEADEIIARTMIPMIIETVRCLEEGIVASPAEADMG 656
            D       AE     +         A+E++AR    +  E    L EG+VA P + D+ 
Sbjct: 613 ADGYLAAPAAELLERTRDPRRAAPATAEELLARVQDALAEEIGTMLAEGVVAGPEDVDLC 672

Query: 657 LVYGLGFPPFRGGVFRYLDTMGVANFV 683
           +V G  +P   GG+  YLD  G A  V
Sbjct: 673 MVLGANYPFHLGGITPYLDRTGAAERV 699


Lambda     K      H
   0.319    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1130
Number of extensions: 76
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 716
Length of database: 707
Length adjustment: 39
Effective length of query: 677
Effective length of database: 668
Effective search space:   452236
Effective search space used:   452236
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources