Alignments for a candidate for pcaF in Novosphingobium fuchskuhlense FNE08-7

GapMind for catabolism of small carbon sources

Alignments for a candidate for pcaF in Novosphingobium fuchskuhlense FNE08-7

Align β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized)
to candidate WP_067913759.1 AQZ52_RS17075 acetyl-CoA C-acyltransferase

Query= metacyc::MONOMER-15952
         (401 letters)



>NCBI__GCF_001519075.1:WP_067913759.1
          Length = 416

 Score =  248 bits (633), Expect = 2e-70
 Identities = 173/436 (39%), Positives = 231/436 (52%), Gaps = 55/436 (12%)

Query: 1   MNEALIIDAVRTPIG---RYAGALASVRADDLGAIPLKALIARHPQLDWSAVDDVIYGCA 57
           M EA IIDAVRTP G      GAL+ +    L A  +KA+  R+  LD   VDD+I+  +
Sbjct: 1   MAEAYIIDAVRTPRGVGKPGKGALSHLHPQHLAATVMKAIAERN-NLDTGTVDDIIWSTS 59

Query: 58  NQAGEDNRNVARMAALLAGLPVSVPGTTLNRLCGSGLDAVGSAARALRCGEAGLMLAGGV 117
           +Q G+   ++ RMAAL AG  ++  GTTL+R CG G+ +V  AA  +  G    ++AGG 
Sbjct: 60  SQVGKQGGDLGRMAALAAGYNITASGTTLDRFCGGGITSVNLAAATVMSGMEDCVIAGGT 119

Query: 118 ESMSRAPFVMGKSEQAFGRSAEIFDTTIGWRFVNKLMQQG-FGIDSM-PETAENVAAQF- 174
           E MS            F  +    +   G  F  +LM  G   +D++ P++ + +     
Sbjct: 120 EMMS------------FTAANAAAEANAG--FPPRLMGSGNAALDALHPQSHQGICGDAI 165

Query: 175 ----NISRADQDAFALRSQHKAAAAIANGRLAKEIVAVEIAQRKGPAKIVEHDEHPRGDT 230
                ISR   DA AL SQ +A  AI  GR  K +V V     +     ++H+E PR +T
Sbjct: 166 ATIEGISREALDALALVSQQRADVAIKEGRFNKSVVPV---LNEDGTVALDHEEFPRPET 222

Query: 231 TLEQLAKLGTPFRQ------GGSVT-------------------AGNASGVNDGACALLL 265
           T E LA L   F        GG +T                   AGN+SGV DGA ALL+
Sbjct: 223 TAEGLASLKPAFAAMMDFDLGGGMTFRKQINRRYPDVDIKPVHHAGNSSGVVDGAAALLI 282

Query: 266 ASSEAAQRHGLKARARVVGMATAGVEPRIMGIGPVPATRKVLELTGLALADMDVIELNEA 325
            S   A++HGLK RAR+V  A  G +P +M   PVPA +KVL   GL   D+DV E+NEA
Sbjct: 283 TSPTYAEKHGLKPRARIVAYANQGDDPTLMLNAPVPAAKKVLAKAGLTKEDIDVWEINEA 342

Query: 326 FAAQGLAVLRELGLADDDERVNPNGGAIALGHPLGMSGARLVTTALHELEERQGRYALCT 385
           FA      +R+L L  D  +VN NGGA+ALGHP+G +G+ L+ TAL ELE   GRY L T
Sbjct: 343 FAVVAEKFIRDLDL--DRAKVNINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVT 400

Query: 386 MCIGVGQGIALIIERI 401
           MC   G   A+IIER+
Sbjct: 401 MCAAGGMAPAIIIERL 416


Lambda     K      H
   0.319    0.134    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 463
Number of extensions: 31
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 401
Length of database: 416
Length adjustment: 31
Effective length of query: 370
Effective length of database: 385
Effective search space:   142450
Effective search space used:   142450
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources