Alignments for a candidate for lctP in Kocuria turfanensis HO-9042

GapMind for catabolism of small carbon sources

Alignments for a candidate for lctP in Kocuria turfanensis HO-9042

Align L-lactate permease (characterized, see rationale)
to candidate WP_062735109.1 AYX06_RS06720 L-lactate permease

Query= uniprot:Q8EIL2
         (545 letters)



>NCBI__GCF_001580365.1:WP_062735109.1
          Length = 556

 Score =  400 bits (1029), Expect = e-116
 Identities = 220/546 (40%), Positives = 332/546 (60%), Gaps = 25/546 (4%)

Query: 11  GSLWLTAIVALLPIVFFFLALTVLKLKGHIAGALTLLIALAVAIITYKMPVSIALASAIY 70
           GS+ L+A+V  +P++ FF+ L  +K + H++GA  LL A+ VA++ + MP+ +AL SA  
Sbjct: 13  GSVALSALVGTVPLLTFFVMLLGVKARAHVSGATALLAAIVVAVLAFGMPLGLALLSATQ 72

Query: 71  GFSYGLWPIAWIIITAVFLYKITVKTGQFEIIRSSVISVTE-DQRLQMLLVGFSFGAFLE 129
           G  YGL+PI WI++ A++ Y++TV +G+FE +R+   ++   D R+Q +L+ F FG  LE
Sbjct: 73  GAVYGLFPIVWIVVMALWFYQVTVLSGRFEDLRTIFDTIGGGDLRIQAVLIAFCFGGLLE 132

Query: 130 GAAGFGAPVAITAALLVGLGFNPLYAAGLCLIANTAPVAFGAMGIPIIVAGQVSSLDPFH 189
             AGFGAPVAITA +++ LG  PL  A + LIANTAPVAFGA+ IPI  AG ++ LDP  
Sbjct: 133 ALAGFGAPVAITATMILALGLKPLKTATVVLIANTAPVAFGAIAIPITTAGVLTGLDPAR 192

Query: 190 IGQLAGRQLPILSIIVPFWLIAMMDGIRGIRQTWPATLVAGVSFAVTQFLTSNFIGPELP 249
           IG + G Q P  +++VPF L+ ++DG+RG+R+ W A LV GV FAV Q+ T+ F   EL 
Sbjct: 193 IGAVVGHQAPFFAVLVPFILLFIIDGVRGLREAWLAALVIGVCFAVAQWWTATFFSYELT 252

Query: 250 DITSALVSLICLTLFLKVWQPKEIFTFSGMKQRAVTPKSTFSNG----QIFKAWSPFIIL 305
           D+ ++LV L    + L+ W+P+      G++ R    K     G    +++ A  P++++
Sbjct: 253 DVVASLVGLAAAVVLLRFWRPRGA---DGVRARLGVDKPPAPEGLTPARVWMAVLPYVLV 309

Query: 306 TA---IVTLWSI-KDVQLALSFATISIEVPYLHNLVIKTAPIVAKETPYAAIYKLNLLGA 361
            A   +  LW++  DV  AL+   +SI  P L   ++        E   + +YKL  L +
Sbjct: 310 VAVFGVAKLWTLGADVPAALAGTDVSIPWPGLDGRLVD----AEGEPLSSTVYKLQWLSS 365

Query: 362 VGTAILIAAMISIVV---------LKMSISNALTSFKDTLIELRFPILSIGLVLAFAFVA 412
            GT +L+  ++  V+          ++S+ +A+     +  ++RF  L+I  VL+ A+V 
Sbjct: 366 PGTLLLLTGLLVAVIYSVFTAGGRFRLSVGDAVVEIGRSFWKMRFSALTIMSVLSLAYVM 425

Query: 413 NYSGLSSTLALVLAGTGVAFPFFSPFLGWLGVFLTGSDTSSNALFGALQANTANQIGVTP 472
           N SG + ++   +AG G AF FFSP LGWLG  +TGSDTS+NALF  LQ   A   G+ P
Sbjct: 426 NTSGQTISIGTWVAGIGAAFAFFSPVLGWLGTAVTGSDTSANALFSNLQKTAAEGTGLDP 485

Query: 473 ELLVAANTTGGVTGKMISPQSIAVACAATGLAGKESDLFRFTLKHSLFFCTFIGVLTVLQ 532
            L+ AANT+GGV GKMISPQS+A+A  A G+ GKES +FR  +  SL     +  L  LQ
Sbjct: 486 HLMTAANTSGGVVGKMISPQSLAIAATAVGMEGKESAIFRSVIWWSLGLLLLLCTLVFLQ 545

Query: 533 AYIVPW 538
           + ++ W
Sbjct: 546 SNVLAW 551


Lambda     K      H
   0.327    0.140    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 914
Number of extensions: 42
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 545
Length of database: 556
Length adjustment: 36
Effective length of query: 509
Effective length of database: 520
Effective search space:   264680
Effective search space used:   264680
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources