GapMind for catabolism of small carbon sources

 

Alignments for a candidate for ivdG in Collimonas arenae Ter10

Align 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Scully protein; Type II HADH; EC 1.1.1.35; EC 1.1.1.51; EC 1.1.1.178 (characterized)
to candidate WP_061534238.1 CAter10_RS16300 3-oxoacyl-ACP reductase FabG

Query= SwissProt::O18404
         (255 letters)



>NCBI__GCF_001584165.1:WP_061534238.1
          Length = 252

 Score = 94.4 bits (233), Expect = 2e-24
 Identities = 79/256 (30%), Positives = 121/256 (47%), Gaps = 23/256 (8%)

Query: 1   MIKNAVSLVTGGASGLGRATAERLAKQGASVILADLPSS-KGNEVAKEL---GDKVVFVP 56
           ++   V+ + GG+ G+G A A+RLA+ GA+V L  + S  +  ++ K++   G + + + 
Sbjct: 9   VLAGKVAFIQGGSRGIGAAVAKRLARDGAAVALTYISSGERALQMVKDIEAAGGRALAIK 68

Query: 57  VDVTSEKDVSAALQTAKDKFGRLDLTVNCAGTATAVKTFNFNKNVAHRLEDFQRVININT 116
            D    + + AA+     KFGRLD+ VN AG        +F+      LEDF R + IN 
Sbjct: 69  ADSADAEAIKAAIGQTMTKFGRLDILVNNAGVLAIAPLADFS------LEDFDRTLAINV 122

Query: 117 VGTFNVIRLSAGLMGANEPNQDGQRGVIVNTASVAAFDGQI-GQAAYSASKAAVVGMTLP 175
              F   + +A  MG          G I+N  S  A      G   Y+ SK+A+VG+T  
Sbjct: 123 RSVFIASQEAARHMGGG--------GRIINIGSTNADRMPFAGGGPYAMSKSALVGLTRG 174

Query: 176 IARDLSTQGIRICTIAPGLFNTPMLAALPEKVRTFLAKSIPFPQRLGEPSEYAHLVQ--A 233
           +ARDL  +GI I  + PG  +T M  A  +   +   K++    R G   E A  V   A
Sbjct: 175 LARDLGPRGITINNVQPGPVDTDMNPAGSDFAASL--KAMMPVGRYGTAEEVAGFVAYLA 232

Query: 234 IYENPLLNGEVIRIDG 249
             E   + G  + IDG
Sbjct: 233 SPEAAYITGASLSIDG 248


Lambda     K      H
   0.317    0.133    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 146
Number of extensions: 8
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 252
Length adjustment: 24
Effective length of query: 231
Effective length of database: 228
Effective search space:    52668
Effective search space used:    52668
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory