Alignments for a candidate for mglA in Collimonas arenae Ter10

GapMind for catabolism of small carbon sources

Alignments for a candidate for mglA in Collimonas arenae Ter10

Align Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized)
to candidate WP_061532689.1 CAter10_RS05910 sugar ABC transporter ATP-binding protein

Query= TCDB::P0AAG8
         (506 letters)



>NCBI__GCF_001584165.1:WP_061532689.1
          Length = 518

 Score =  434 bits (1116), Expect = e-126
 Identities = 221/496 (44%), Positives = 330/496 (66%), Gaps = 5/496 (1%)

Query: 11  EYLLEMSGINKSFPGVKALDNVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKDSGTI 70
           EYL+ +  + K FPGV ALDN    +    +HALMGENGAGKSTL+K L G+Y KDSG I
Sbjct: 17  EYLIALKNVTKRFPGVLALDNCQFNLLRGEVHALMGENGAGKSTLMKVLSGVYPKDSGEI 76

Query: 71  LFQGKEIDFHSAKEALENGISMVHQELNLVLQRSVMDNMWLGRYPTK--GMFVDQDKMYR 128
              G+ ++  + + A   GI ++HQELNL+   S   N+++GR P    G+F+D+D + R
Sbjct: 77  RMDGRPVEIPNPRAAQALGIGIIHQELNLMNHLSAAQNIFIGREPRGRYGVFLDEDALNR 136

Query: 129 ETKAIFDELDIDIDPRARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSSLTEKEVNHL 188
           +   IF+ + + +DPR  VG L+V++ QM+EIAKA S++++++IMDEPT++L   E++ L
Sbjct: 137 QAAQIFERMRLQLDPRTLVGELTVAKQQMVEIAKALSFDSRVLIMDEPTAALNNAEIDDL 196

Query: 189 FTIIRKLKERGCGIVYISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLTMDKIIAMMVGR 248
           F IIR+L+E G GI+YISHKM+E+ Q+ + VTV+RDGQ+IAT P  G  +D II MMVGR
Sbjct: 197 FRIIRQLQEHGVGIIYISHKMDELRQISNRVTVMRDGQYIATVPTVGTPIDTIIGMMVGR 256

Query: 249 SLNQRFPD-KENKPGEVILEVRNLTSLRQPSIRDVSFDLHKGEILGIAGLVGAKRTDIVE 307
            L+   P+  +    +V+LEV+ LT  R  +I+DV+F L KGEILG AGL+GA RT++  
Sbjct: 257 QLDNSGPEVPDTSANDVVLEVKGLT--RGAAIKDVNFSLRKGEILGFAGLMGAGRTEVAR 314

Query: 308 TLFGIREKSAGTITLHGKQINNHNANEAINHGFALVTEERRSTGIYAYLDIGFNSLISNI 367
            +FG     AG I +HG +++  +  +A+ HG   ++E+R+  G+   LD+  N ++S++
Sbjct: 315 AVFGADAIDAGEILVHGVKVSIKSPRDAVAHGIGYLSEDRKHFGLATGLDVKTNVVMSSM 374

Query: 368 RNYKNKVGLLDNSRMKSDTQWVIDSMRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEI 427
             +  K   LD   ++   Q  +  + +KTP     +  LSGGNQQK++I +WLL   +I
Sbjct: 375 DKFLTKGLFLDQPAIRETAQGYVRQLSIKTPSIDQPVRLLSGGNQQKIVIAKWLLRDCDI 434

Query: 428 LMLDEPTRGIDVGAKFEIYQLIAELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGI 487
           L  DEPTRGID+GAK EIY+L+  LA +GK I++ISSE+PE+L ++ RILVM  G ++G 
Sbjct: 435 LFFDEPTRGIDIGAKNEIYKLLNALAAQGKAIVMISSELPEVLRMSHRILVMCEGRITGE 494

Query: 488 VDTKTTTQNEILRLAS 503
           +     +Q +I+ LA+
Sbjct: 495 LAAADASQEKIMYLAT 510



 Score = 87.4 bits (215), Expect = 1e-21
 Identities = 64/250 (25%), Positives = 119/250 (47%), Gaps = 15/250 (6%)

Query: 8   SSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKDS 67
           S+ + +LE+ G+ +      A+ +VN  +R   I    G  GAG++ + + +FG    D+
Sbjct: 269 SANDVVLEVKGLTRG----AAIKDVNFSLRKGEILGFAGLMGAGRTEVARAVFGADAIDA 324

Query: 68  GTILFQGKEIDFHSAKEALENGISMVHQE---LNLVLQRSVMDNMWLG---RYPTKGMFV 121
           G IL  G ++   S ++A+ +GI  + ++     L     V  N+ +    ++ TKG+F+
Sbjct: 325 GEILVHGVKVSIKSPRDAVAHGIGYLSEDRKHFGLATGLDVKTNVVMSSMDKFLTKGLFL 384

Query: 122 DQDKMYRETKAIFDELDI---DIDPRARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTS 178
           DQ  +    +    +L I    ID   R+  LS    Q I IAK    +  I+  DEPT 
Sbjct: 385 DQPAIRETAQGYVRQLSIKTPSIDQPVRL--LSGGNQQKIVIAKWLLRDCDILFFDEPTR 442

Query: 179 SLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLTM 238
            +     N ++ ++  L  +G  IV IS ++ E+ ++   + V+ +G+       A  + 
Sbjct: 443 GIDIGAKNEIYKLLNALAAQGKAIVMISSELPEVLRMSHRILVMCEGRITGELAAADASQ 502

Query: 239 DKIIAMMVGR 248
           +KI+ +   R
Sbjct: 503 EKIMYLATQR 512


Lambda     K      H
   0.318    0.136    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 615
Number of extensions: 29
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 506
Length of database: 518
Length adjustment: 35
Effective length of query: 471
Effective length of database: 483
Effective search space:   227493
Effective search space used:   227493
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources