GapMind for catabolism of small carbon sources

 

Alignments for a candidate for hisM in Collimonas arenae Ter10

Align Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter membrane protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate WP_061533332.1 CAter10_RS10280 amino acid ABC transporter permease

Query= TCDB::Q9HU29
         (230 letters)



>NCBI__GCF_001584165.1:WP_061533332.1
          Length = 250

 Score =  110 bits (276), Expect = 2e-29
 Identities = 76/242 (31%), Positives = 134/242 (55%), Gaps = 29/242 (11%)

Query: 3   EWELILKWMPKMLQGAALTLELLAIAVVAGLALALPLGIARAS--RH----WYVR-AVPY 55
           +W++I +++P  ++G  +TL++  I VV G  L L LG+ R +  RH    +++R AV +
Sbjct: 6   QWQIIGEYLPLFIEGTLMTLKVAVICVVTGTFLGLLLGVGRVAEARHGAMKYFLRYAVQW 65

Query: 56  A---YIFFFRGTPLLLQLFIVYY-----------GLAQFEEVRKSAFWPYLRDPYWCALL 101
               Y+ FFRGTPL +Q+ ++++           GL    ++ +     Y    +  A+L
Sbjct: 66  PVRFYVSFFRGTPLFVQILLIHFALMPLLINPNGGLLLSGDIAREVRSQY--GAFLSAVL 123

Query: 102 TMTLHTAAYIAEILRGAIHSVPVGEVEAARALGMSRRQALWHIILPRAVRIGLPAYSNEV 161
            +TL++ AY++EI R  I S+  G+ EA+R+LGM+  Q L  ++LP+A R  LP   N  
Sbjct: 124 AITLNSGAYVSEIFRAGIQSIDRGQSEASRSLGMTYLQTLRKVVLPQAFRRMLPPLGNNA 183

Query: 162 ILMLKASAVVYTVTLFDIMGMARTI---IARTYESMLFFCLAGALYLVITIVLTRIFRLI 218
           I ++K S++   + L ++   ART+    AR +E  L   L   +Y  IT++L+   + +
Sbjct: 184 IAIVKDSSLASAIGLAELAYAARTVSGAYARYWEPYLTISL---IYWGITLLLSVFVQHL 240

Query: 219 ER 220
           E+
Sbjct: 241 EK 242


Lambda     K      H
   0.332    0.142    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 142
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 230
Length of database: 250
Length adjustment: 23
Effective length of query: 207
Effective length of database: 227
Effective search space:    46989
Effective search space used:    46989
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory