Alignments for a candidate for kynA in Collimonas arenae Ter10

GapMind for catabolism of small carbon sources

Alignments for a candidate for kynA in Collimonas arenae Ter10

Align Tryptophan 2,3-dioxygenase (EC 1.13.11.11) (characterized)
to candidate WP_061532529.1 CAter10_RS04900 tryptophan 2,3-dioxygenase

Query= reanno::Cup4G11:RR42_RS15390
         (291 letters)



>NCBI__GCF_001584165.1:WP_061532529.1
          Length = 280

 Score =  403 bits (1035), Expect = e-117
 Identities = 194/285 (68%), Positives = 229/285 (80%), Gaps = 7/285 (2%)

Query: 7   CPMSGAGATETSDASWHDAQMDFAKDMSYGDYLALDQILNAQHPLSPEHNEMLFIVQHQT 66
           CP +  GA       WH A+M+F +DMSYGDYLALDQ+L+AQ PLSP HNEMLFIVQHQT
Sbjct: 3   CPYNEKGA-------WHGAKMEFKEDMSYGDYLALDQVLSAQRPLSPNHNEMLFIVQHQT 55

Query: 67  SELWMKLALHELRAARECVRQDQLPPAFKMLTRVSRIMEQLVQAWNVLATMTPPEYSAMR 126
           SELWMKL LHEL A R  +R + L PAFKM+ RV+RIM+QLV AW+VLATMTPPEY+A+R
Sbjct: 56  SELWMKLMLHELHAVRAQLRMNMLSPAFKMMARVARIMDQLVHAWDVLATMTPPEYTAIR 115

Query: 127 PYLGMSSGFQSFQYREIEFILGNKNAAMLRPHAHQPQHLALLEEALRTPSLYDEAIRLMA 186
           PYLG SSGFQS+QYREIEF+LGNKNA +L+ H   P+  A L++AL+ PS+YDEA+ LMA
Sbjct: 116 PYLGHSSGFQSYQYREIEFLLGNKNATLLQMHRDSPESFARLDDALKQPSIYDEAVMLMA 175

Query: 187 RRGFAIDAACIERDWTRPAGENASVEAAWLEVYRKPEAHWELYELGEKFVDLEDAFRQWR 246
           R G A+D A +  DW+RP   + SVE AWL VY+ P+ HWELYEL EK VDLE +FR WR
Sbjct: 176 RAGLAVDDARLALDWSRPTVADQSVENAWLTVYKTPDKHWELYELAEKLVDLETSFRFWR 235

Query: 247 FRHVTTVERVIGFKRGTGGTEGVSYLRKMLDVVLFPELWKLRTDL 291
           FRH+TTVERVIGFK+GTGGTEG SYL+KMLDVVLFPEL+ LRT L
Sbjct: 236 FRHLTTVERVIGFKKGTGGTEGASYLKKMLDVVLFPELFSLRTSL 280


Lambda     K      H
   0.322    0.134    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 351
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 291
Length of database: 280
Length adjustment: 26
Effective length of query: 265
Effective length of database: 254
Effective search space:    67310
Effective search space used:    67310
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

Align candidate WP_061532529.1 CAter10_RS04900 (tryptophan 2,3-dioxygenase)
to HMM TIGR03036 (kynA: tryptophan 2,3-dioxygenase (EC 1.13.11.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03036.hmm
# target sequence database:        /tmp/gapView.817476.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03036  [M=264]
Accession:   TIGR03036
Description: trp_2_3_diox: tryptophan 2,3-dioxygenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   2.5e-128  413.0   1.0   2.8e-128  412.8   1.0    1.0  1  NCBI__GCF_001584165.1:WP_061532529.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_001584165.1:WP_061532529.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  412.8   1.0  2.8e-128  2.8e-128       1     264 []      17     280 .]      17     280 .] 1.00

  Alignments for each domain:
  == domain 1  score: 412.8 bits;  conditional E-value: 2.8e-128
                             TIGR03036   1 dfsesmsYgdYlkldellsaqkplsedhdemlfivqhqvselwlklilheleaaaralradeleaalkalaRv 73 
                                           +f+e+msYgdYl+ld++lsaq+pls++h+emlfivqhq+selw+kl+lhel+a +++lr + l++a+k++aRv
  NCBI__GCF_001584165.1:WP_061532529.1  17 EFKEDMSYGDYLALDQVLSAQRPLSPNHNEMLFIVQHQTSELWMKLMLHELHAVRAQLRMNMLSPAFKMMARV 89 
                                           69*********************************************************************** PP

                             TIGR03036  74 srileqlveaWdvLatltPaeysefRealgessGfqsyqyReiefllGnknaallkphekdpellaeleaale 146
                                           +ri++qlv+aWdvLat+tP ey+++R++lg+ssGfqsyqyReiefllGnkna+ll++h+++pe  a+l++al+
  NCBI__GCF_001584165.1:WP_061532529.1  90 ARIMDQLVHAWDVLATMTPPEYTAIRPYLGHSSGFQSYQYREIEFLLGNKNATLLQMHRDSPESFARLDDALK 162
                                           ************************************************************************* PP

                             TIGR03036 147 ePslYdevlrllarrGfaipaevlerdvtkpaeaneeveaawlevYrdaekewelyelaeklvDledlfrrWR 219
                                           +Ps+Yde++ l+ar+G+a+++++l  d+++p+ a+++ve+awl+vY+ ++k+welyelaeklvDle +fr WR
  NCBI__GCF_001584165.1:WP_061532529.1 163 QPSIYDEAVMLMARAGLAVDDARLALDWSRPTVADQSVENAWLTVYKTPDKHWELYELAEKLVDLETSFRFWR 235
                                           ************************************************************************* PP

                             TIGR03036 220 frhlttveRiiGfkrGtGGssGvayLkkaldvelfPelwkvRtel 264
                                           frhlttveR+iGfk+GtGG++G++yLkk+ldv+lfPel+++Rt+l
  NCBI__GCF_001584165.1:WP_061532529.1 236 FRHLTTVERVIGFKKGTGGTEGASYLKKMLDVVLFPELFSLRTSL 280
                                           *******************************************86 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (264 nodes)
Target sequences:                          1  (280 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 21.84
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources