Alignments for a candidate for malK_Ss in Collimonas pratensis Ter91

GapMind for catabolism of small carbon sources

Alignments for a candidate for malK_Ss in Collimonas pratensis Ter91

Align MalK, component of Maltose and maltooligosaccharide porter (characterized)
to candidate WP_061946031.1 CPter91_RS08965 ABC transporter ATP-binding protein

Query= TCDB::Q97UG5
         (617 letters)



>NCBI__GCF_001584185.1:WP_061946031.1
          Length = 328

 Score =  185 bits (469), Expect = 3e-51
 Identities = 102/259 (39%), Positives = 169/259 (65%), Gaps = 5/259 (1%)

Query: 355 VVMKILNLSKIYYIRKNLILSEPI-NAVNDVSFELKKGTITALVGGSGHGKSTIAKILAG 413
           V+++  NL K Y + + L   +    A++ VSF L+ G   A+VG SG GKST+A+ +  
Sbjct: 9   VLLEAKNLVKHYSVSQGLFKPKATARALDGVSFALQPGKTLAVVGESGCGKSTLARQITM 68

Query: 414 MIQQTSGKIILLGKDVSEYGVRNSMWYKENVQMIFQDPYSSLDPRHTVRWHVERPLLIHK 473
           +   T G++ + G ++++         +  VQM+FQ+PY+SL+PR  V   +E PL+I+ 
Sbjct: 69  IEPPTGGELWMDGANIADADHATLKRVRPLVQMVFQNPYASLNPRKKVGQMLEEPLIINT 128

Query: 474 KVSNKDQLLPKIIEVLKNVGLKPPEKYLYKYPHELSGGERQRVAIARATAVEPKVLVADE 533
            +++ ++   K   ++  VGL+P  ++  +YPH  SGG+RQRVAIARA  ++PKV+VADE
Sbjct: 129 GLNSAERA-EKARAMMAKVGLRP--EHYSRYPHMFSGGQRQRVAIARALMLDPKVIVADE 185

Query: 534 PVSMLDASLRAGILNLIKKFKK-NGISILYITHDIATVNYIADEIMVIYKGRIVEKGNTY 592
           PVS LD S++A +LNL+   ++ +G++ L+I+H+++ V +IADE++V+Y G+ VE G+  
Sbjct: 186 PVSALDVSIQAQVLNLLMDLQQASGVAYLFISHNLSVVEHIADEVLVMYLGKTVEHGSKQ 245

Query: 593 EVISNPSHEYTKRLIEAVP 611
           +V S P H YTK L+ + P
Sbjct: 246 QVFSRPLHPYTKALLASTP 264



 Score =  175 bits (443), Expect = 3e-48
 Identities = 106/315 (33%), Positives = 174/315 (55%), Gaps = 11/315 (3%)

Query: 7   SLLKVNELTAGYFNQDGFVIGVTNVNFEVYPNEIFAIAGESGCGKSTLAMAIYGLLKYPG 66
           +L+K   ++ G F        +  V+F + P +  A+ GESGCGKSTLA  I  +    G
Sbjct: 15  NLVKHYSVSQGLFKPKATARALDGVSFALQPGKTLAVVGESGCGKSTLARQITMIEPPTG 74

Query: 67  VVLRGHVYLKDKDILSITQEELRKLR-MKEFVYVPQFAMDALDPVAKIGDQMMRAAVSH- 124
               G +++   +I       L+++R + + V+   +A  +L+P  K+G  +    + + 
Sbjct: 75  ----GELWMDGANIADADHATLKRVRPLVQMVFQNPYA--SLNPRKKVGQMLEEPLIINT 128

Query: 125 GVNVEEARKLIKEKLELVDLPYNVVNMYPHELSGGMRQRVVIATSILLNPSLIILDEPTT 184
           G+N  E  +  +  +  V L     + YPH  SGG RQRV IA +++L+P +I+ DEP +
Sbjct: 129 GLNSAERAEKARAMMAKVGLRPEHYSRYPHMFSGGQRQRVAIARALMLDPKVIVADEPVS 188

Query: 185 GLDVIVQYEILKDLKRIQRQLGVSLVIISHDISMLLMISDRVGIMYAGEIVEIGSKEEII 244
            LDV +Q ++L  L  +Q+  GV+ + ISH++S++  I+D V +MY G+ VE GSK+++ 
Sbjct: 189 ALDVSIQAQVLNLLMDLQQASGVAYLFISHNLSVVEHIADEVLVMYLGKTVEHGSKQQVF 248

Query: 245 KRPSHPYTYLLISSLPSL-VKRREKLLSIPGNPPLMLSKVPNSCRFYDRCPFKMEKCSTL 303
            RP HPYT  L++S P +   +R++ + +PG  P  L+  P  C F +RCP  +E+C   
Sbjct: 249 SRPLHPYTKALLASTPRIDPAQRQQKMMLPGELPSPLAP-PPGCSFNNRCPHAIERCRQE 307

Query: 304 NPALGDIMDGHKARC 318
            PAL    DG    C
Sbjct: 308 VPALRS-FDGRLVAC 321


Lambda     K      H
   0.319    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 521
Number of extensions: 22
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 617
Length of database: 328
Length adjustment: 33
Effective length of query: 584
Effective length of database: 295
Effective search space:   172280
Effective search space used:   172280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources