GapMind for catabolism of small carbon sources

 

Alignments for a candidate for braG in Methanoculleus horonobensis T10

Align High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate WP_067072406.1 MCUHO_RS00905 ATP-binding cassette domain-containing protein

Query= TCDB::P21630
         (233 letters)



>NCBI__GCF_001602375.1:WP_067072406.1
          Length = 355

 Score =  120 bits (301), Expect = 3e-32
 Identities = 79/233 (33%), Positives = 130/233 (55%), Gaps = 12/233 (5%)

Query: 1   MLSFDKVSTYYGKIQALHDVSVEVKKGEIVTLIGANGAGKSTLLMTLCGSPQAASGSIRY 60
           M+ FD+VS   G  + L+DVS+ + KG+   ++G +GAGK+ LL  + G  +  SG +  
Sbjct: 1   MIEFDRVSLALGSFR-LNDVSLTISKGDYYFIVGPSGAGKTVLLEAIAGLHRPDSGRVLL 59

Query: 61  EGEELVGLPSSTIMRKSIAVVPEGRRVFSRLTVEENLAMGGFFTD--KDDYQVQMDKVLE 118
            GEE+  LP     ++++A+V +   +F  + V +N++ G       K + + ++  +LE
Sbjct: 60  RGEEITALPPE---KRNVALVYQDYSLFPNMRVIDNISYGLRVQGMGKKEARAEVAPLLE 116

Query: 119 LF--PRLKERYEQRAGTMSGGEQQMLAIGRALMSKPKLLLLDEPSLGLAPIIIQQIFEII 176
            F    L +RY    GT+SGGEQQ +A+ RA+  KP +LLLDEP   L P+  ++  + +
Sbjct: 117 RFGIAHLADRYP---GTLSGGEQQRVALARAVAVKPDILLLDEPLSALDPVTQEKFIDDL 173

Query: 177 EQLRRE-GVTVFLVEQNANQALKLADRAYVLENGRIVMHDTGAALLTNPKVRD 228
            +L RE G+TV  V  +  +A  LA R  V+ +G +V       +L  PK R+
Sbjct: 174 RRLHREDGLTVVQVSHSRREAHLLATRMAVIIDGALVDEGKADVVLNAPKSRE 226


Lambda     K      H
   0.318    0.136    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 158
Number of extensions: 5
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 233
Length of database: 355
Length adjustment: 26
Effective length of query: 207
Effective length of database: 329
Effective search space:    68103
Effective search space used:    68103
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory