Alignments for a candidate for nupA in Pseudovibrio axinellae Ad2

GapMind for catabolism of small carbon sources

Alignments for a candidate for nupA in Pseudovibrio axinellae Ad2

Align Purine/cytidine ABC transporter ATP-binding protein, component of General nucleoside uptake porter, NupABC/BmpA (transports all common nucleosides as well as 5-fluorocytidine, inosine, deoxyuridine and xanthosine) (Martinussen et al., 2010) (Most similar to 3.A.1.2.12). NupA is 506aas with two ABC (C) domains. NupB has 8 predicted TMSs, NupC has 9 or 10 predicted TMSs in a 4 + 1 (or 2) + 4 arrangement (characterized)
to candidate WP_068000557.1 PsAD2_RS00555 sugar ABC transporter ATP-binding protein

Query= TCDB::A2RKA7
         (506 letters)



>NCBI__GCF_001623255.1:WP_068000557.1
          Length = 522

 Score =  290 bits (742), Expect = 9e-83
 Identities = 169/503 (33%), Positives = 287/503 (57%), Gaps = 9/503 (1%)

Query: 3   NETVIQMIDVTKRFGDFVANDKVNLELKKGEIHALLGENGAGKSTLMNILSGLLEPSEGE 62
           ++  + +  V K FG   A D V+  LK+GE+HAL+GENGAGKSTL+ ILSG+     G 
Sbjct: 15  DQVFLDVQGVKKYFGGVKALDGVSFSLKQGEVHALVGENGAGKSTLIKILSGVYSFDSGN 74

Query: 63  VHVKGKLENIDSPSKAANLGIGMVHQHFMLVDAFTVTENIILGNEVTKGINLDLK-TAKK 121
           + ++      +SP +A   GI +VHQ F L++  ++ ENI +         L  K    +
Sbjct: 75  ISLENTSYQPESPQEAKARGIQVVHQEFNLLEHLSIAENISIERLPRNKYGLLNKGEMNR 134

Query: 122 KILELSERYGLS-VEPDALIRDISVGQQQRVEILKTLYRGADILIFDEPTAVLTPAEITE 180
           +  E  +  GL+ ++    +  + +  +Q +EI + L   + ILI DEPTA LT  E   
Sbjct: 135 RAREALDAIGLTDIDVRVPVGSLGIAHRQLIEIARALQSKSQILILDEPTATLTERETKR 194

Query: 181 LMQIMKNLIKEGKSIILITHKLDEIRAVADRITVIRRGKSIDTVELGDKTNQELAELMVG 240
           L +I+  +  EG +++ ++H LDE+ A+ DR+TV R GK++ T ++   T + + + MVG
Sbjct: 195 LFKIIAAIKSEGVTVVFVSHHLDEVFAICDRVTVFRNGKTVATDKISHMTPEGVVQRMVG 254

Query: 241 RSVSFITEKAAAQPK--DVVLEIKDLNIKESRGSLKVKGLSLDVRAGEIVGVAGIDGNGQ 298
           R +   T +   +     V L+I  +   ++ G     G+SL++  GEIVG+AG+ G+G+
Sbjct: 255 RHLEAGTRQHTDKQTFGPVALQIDAMRTMQNTGDT---GISLNLHYGEIVGIAGLVGSGR 311

Query: 299 TELVKAITGLTKVDSGSIKLHNKDITNQRPRKITEQSVGHVPEDRHRDGLVLEMTVAENI 358
           +E+++ I G+  + SG++    +++  + P+   +  +G V EDR  +GL+L+M +A N 
Sbjct: 312 SEILRGIFGIDPIHSGTVYRDGEEVNFKGPQDAIKAGIGFVTEDRKDEGLILDMPIAANT 371

Query: 359 ALQTYYKPPMSKYGFLDYNKINSHARELMEEFDVRGAGEWVSASSLSGGNQQKAIIAREI 418
           +L   ++  +SK G + + + N  ARE      ++       ASSLSGGNQQK ++A+ +
Sbjct: 372 SLVNIHE--LSKTGLIQFTEENRQARESGSRLKLKYGKTADPASSLSGGNQQKVVLAKWL 429

Query: 419 DRNPDLLIVSQPTRGLDVGAIEYIHKRLIQARDEGKAVLVISFELDEILNVSDRIAVIHD 478
             NP +L++ +PTRG+DVGA   I+  L     EG A+LV+S E+ E++ ++DRI V+ +
Sbjct: 430 ACNPKVLLLDEPTRGVDVGAKAEIYSILKDLAQEGVALLVVSSEMPELMTLADRIVVLAE 489

Query: 479 GQIQGIVSPETTTKQELGILMVG 501
             IQG + P   +++ +  L  G
Sbjct: 490 HAIQGELKPSEFSEENILKLAYG 512



 Score = 75.9 bits (185), Expect = 3e-18
 Identities = 57/227 (25%), Positives = 111/227 (48%), Gaps = 12/227 (5%)

Query: 25  VNLELKKGEIHALLGENGAGKSTLMNILSGLLEPSEGEVHVKGKLENIDSPSKAANLGIG 84
           ++L L  GEI  + G  G+G+S ++  + G+     G V+  G+  N   P  A   GIG
Sbjct: 291 ISLNLHYGEIVGIAGLVGSGRSEILRGIFGIDPIHSGTVYRDGEEVNFKGPQDAIKAGIG 350

Query: 85  MVHQHFM---LVDAFTVTENIILGN--EVTKG----INLDLKTAKKKILELSERYGLSVE 135
            V +      L+    +  N  L N  E++K        + + A++    L  +YG + +
Sbjct: 351 FVTEDRKDEGLILDMPIAANTSLVNIHELSKTGLIQFTEENRQARESGSRLKLKYGKTAD 410

Query: 136 PDALIRDISVGQQQRVEILKTLYRGADILIFDEPTAVLTPAEITELMQIMKNLIKEGKSI 195
           P +    +S G QQ+V + K L     +L+ DEPT  +      E+  I+K+L +EG ++
Sbjct: 411 PAS---SLSGGNQQKVVLAKWLACNPKVLLLDEPTRGVDVGAKAEIYSILKDLAQEGVAL 467

Query: 196 ILITHKLDEIRAVADRITVIRRGKSIDTVELGDKTNQELAELMVGRS 242
           ++++ ++ E+  +ADRI V+        ++  + + + + +L  G++
Sbjct: 468 LVVSSEMPELMTLADRIVVLAEHAIQGELKPSEFSEENILKLAYGQA 514


Lambda     K      H
   0.315    0.135    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 660
Number of extensions: 33
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 506
Length of database: 522
Length adjustment: 35
Effective length of query: 471
Effective length of database: 487
Effective search space:   229377
Effective search space used:   229377
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources