Alignments for a candidate for pimF in Dokdonella koreensis DS-123

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Dokdonella koreensis DS-123

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate WP_067643566.1 I596_RS02240 3-hydroxyacyl-CoA dehydrogenase

Query= metacyc::MONOMER-20678
         (699 letters)



>NCBI__GCF_001632775.1:WP_067643566.1
          Length = 682

 Score =  279 bits (714), Expect = 3e-79
 Identities = 216/692 (31%), Positives = 334/692 (48%), Gaps = 61/692 (8%)

Query: 11  DQVAIVTVD--SPPVNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGADITEF 68
           + +A +T+D    PVNALS AV     + V     +P    ++       F+ GAD+ EF
Sbjct: 17  EDIATLTLDRAGSPVNALSRAVLDEFDQIVERLSFEPPRGLVIRSGKSAGFVVGADLKEF 76

Query: 69  GKPPQPPALNDVI----AALENSPK---PTIAAIHGTALGGGLEVALGCHFRVAVKEA-- 119
               Q  ++ D I    A  E   +   PT  AIHG  +GGG E++L C++R+A  +A  
Sbjct: 77  AGYEQGGSVIDQIRNGQAVFERLARLRCPTAVAIHGHCMGGGTELSLACNYRIASDDAST 136

Query: 120 KLGLPEVKLGLLPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVV--ENL 177
           ++GLPEVKLG+ PG GG+ RLPR +G   A++ ++ G    AA A   GLV+ +   E L
Sbjct: 137 RIGLPEVKLGIFPGWGGSARLPRLIGAPKALEFMLTGRTASAASARALGLVDAIATPERL 196

Query: 178 VAGAVAFAKKVLAEKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMTKKARGLEAPFACAD 237
           +  A  + ++  A     R L    +     +A   +     AA  +      APFA  +
Sbjct: 197 LDVAKEWVRRRPAPPFGRRALAWATNLWPVRQALAPVLRKQTAAKARPEH-YPAPFALIE 255

Query: 238 AI---GAAIDLPFEEGLKKEREGFMKLVVSDQSKAQ-RYAFFAEREAAKVDGVPDGTKPR 293
                G++I     + L  E     KL  +   +   R  F  ER  A+  GV  G    
Sbjct: 256 TWRRGGSSI----RQRLDLEARAVAKLAQTPTCRNLIRVFFLQERLKAQGAGVEHG---- 307

Query: 294 PVSRVAIIGAGTMGGGIAMSFANAGIPVTLIETGEEQLK----RGLGIMQKNWEATAARG 349
            + R+ ++GAG MGG IA   A  G  VTL +  +  ++    RG  +  K   A     
Sbjct: 308 -IVRIHVVGAGVMGGDIAAWAALRGFEVTLQDREDAYVRPAIERGRALFAKKLRA----- 361

Query: 350 GLPPDAPAKRMALITGLVGLENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASN 409
             P    A    L+  + G   V +ADL+IEA+FE +  K+ ++  ++   KPGA+LASN
Sbjct: 362 --PERVEAASRRLVADVEGA-GVAEADLVIEAIFENLEAKQALYRQLEPRMKPGALLASN 418

Query: 410 TSYLSIDEIAATTKRPQDVLGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKV 469
           TS + +DE+ A    P   LG+H+F+P  +M L EIVR  +  P A   A ++ K + K+
Sbjct: 419 TSSIPLDELRAAVATPGRFLGLHYFNPVALMPLVEIVRHDRLDPAAEKRAAALCKALDKL 478

Query: 470 PVVVGVCDGFVGNRMLAARSKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLD- 528
           P+ V    GF+ NR+L     ++ ++L EG     +D V  KFGMPMGP  + D  GLD 
Sbjct: 479 PIAVAGTPGFLVNRILMPYLLEAIRILKEGVPAPVIDKVARKFGMPMGPIELADTIGLDV 538

Query: 529 ---IGWRSRKDRGIK-SEIADALCEAGRFGQKTGKGYYKYEQGSRAPMPDPEVETLINDT 584
              +G       G+   E  D   EAG  G+KTG+G+Y++E G       PEV+      
Sbjct: 539 CASVGRELAPFLGLDLPEGIDTQLEAGHRGKKTGQGFYRWENGKPV---KPEVDP----- 590

Query: 585 LAKLGLKRRDITDEEILERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIYRGGP 644
                   + +   ++ +R++ P++NE    L +++      +D   ++G G+  +RGGP
Sbjct: 591 --------KYVAPADLEDRLILPLLNEAVACLHDRVVDDADLLDAGVIFGTGFAPFRGGP 642

Query: 645 MHYADSVGLKHIAERLSAYAKATNDPSLEPAP 676
           + +  +VG   +  RL A A A + P     P
Sbjct: 643 IAHIRAVGAATLEARLGALA-ARHGPRFTARP 673


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1097
Number of extensions: 53
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 682
Length adjustment: 39
Effective length of query: 660
Effective length of database: 643
Effective search space:   424380
Effective search space used:   424380
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources