GapMind for catabolism of small carbon sources

 

Alignments for a candidate for fadB in Dokdonella koreensis DS-123

Align GDP-6-deoxy-D-talose 4-dehydrogenase (EC 1.1.1.135); 3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC 1.1.1.178) (characterized)
to candidate WP_067643684.1 I596_RS02455 3-hydroxybutyrate dehydrogenase

Query= BRENDA::Q99714
         (261 letters)



>NCBI__GCF_001632775.1:WP_067643684.1
          Length = 260

 Score =  100 bits (248), Expect = 4e-26
 Identities = 70/210 (33%), Positives = 106/210 (50%), Gaps = 15/210 (7%)

Query: 7   SVKGLVAVITGGASGLGLATAERLVGQGASAVLLDLPNSGGEAQAKKL---GNNCVFAPA 63
           S+ G VA ITG ASGLG   AE     GA+  + DL   G +A A +L   G   +    
Sbjct: 2   SLSGKVAYITGAASGLGKRIAEVYAANGAAVAIADLNLEGAQAAAAELAATGAKAIGVKV 61

Query: 64  DVTSEKDVQTALALAKGKFGRVDVAVNCAGIAVASKTYNLKKGQTHTLEDFQRVLDVNLM 123
           DVTSE +V+ ++A     FG+VD+ V+ AGI +      +K  Q  T E+++R++ ++L 
Sbjct: 62  DVTSEAEVEASVAETIKAFGKVDILVSNAGIQI------VKPVQDFTYEEWRRMIAIHLD 115

Query: 124 GTFNVIRLVAGEMGQNEPDQGGQRGVIINTASVAAFEGQVGQAAYSASKGGIVGMTLPIA 183
           G F   R    +M        GQ G II   S  +      +A Y A+K  ++G+   +A
Sbjct: 116 GAFLTTRACLKDMYAR-----GQGGAIIYMGSAHSKTASKLKAPYVAAKHALLGLARTVA 170

Query: 184 RDLAPIGIRVMTIAPGLFGTPLL-TSLPEK 212
           ++  P  +R   I PG   TPL+   +PE+
Sbjct: 171 KEGGPAKVRANVICPGFVRTPLVEKQIPEQ 200


Lambda     K      H
   0.318    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 146
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 261
Length of database: 260
Length adjustment: 25
Effective length of query: 236
Effective length of database: 235
Effective search space:    55460
Effective search space used:    55460
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory