Alignments for a candidate for paaH in Dokdonella koreensis DS-123

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaH in Dokdonella koreensis DS-123

Align FadB (EC 5.3.3.8; EC 1.1.1.35; EC 5.1.2.3; EC 4.2.1.17; EC 1.1.1.36) (characterized)
to candidate WP_067643566.1 I596_RS02240 3-hydroxyacyl-CoA dehydrogenase

Query= metacyc::G1G01-2277-MONOMER
         (715 letters)



>NCBI__GCF_001632775.1:WP_067643566.1
          Length = 682

 Score =  363 bits (933), Expect = e-104
 Identities = 228/676 (33%), Positives = 352/676 (52%), Gaps = 45/676 (6%)

Query: 17  IVELKFDLKGESVNKFNRLTLNELRQAVDAIRADASVKGVIVRSGKDV-FIVGADITEFV 75
           I  L  D  G  VN  +R  L+E  Q V+ +  +   +G+++RSGK   F+VGAD+ EF 
Sbjct: 19  IATLTLDRAGSPVNALSRAVLDEFDQIVERLSFEPP-RGLVIRSGKSAGFVVGADLKEFA 77

Query: 76  DNFKLPEAELVAGNLEANRIFNAFEDLEVPTVAAINGIALGGGLEMCLAADYRVMS--TS 133
              +     ++        +F     L  PT  AI+G  +GGG E+ LA +YR+ S   S
Sbjct: 78  GYEQ--GGSVIDQIRNGQAVFERLARLRCPTAVAIHGHCMGGGTELSLACNYRIASDDAS 135

Query: 134 ARIGLPEVKLGIYPGFGGTVRLPRLIGSDNAIEWIAAGKENRAEDALKVGAVDAVVAPEL 193
            RIGLPEVKLGI+PG+GG+ RLPRLIG+  A+E++  G+   A  A  +G VDA+  PE 
Sbjct: 136 TRIGLPEVKLGIFPGWGGSARLPRLIGAPKALEFMLTGRTASAASARALGLVDAIATPER 195

Query: 194 LLAGALDLIKRAISGELDYKAKRQPKLEKLKLNAIEQMMAFETAKGFVAGQAGPNYPAPV 253
           LL  A + ++R  +     +A          L  + Q +A    K   A     +YPAP 
Sbjct: 196 LLDVAKEWVRRRPAPPFGRRALAWAT----NLWPVRQALAPVLRKQTAAKARPEHYPAPF 251

Query: 254 EAIKSIQKAANFGRDKALEVEAAGFAKLAKTSVAESLIGLFLNDQELKRKAKAHDEIAHD 313
             I++ ++  +  R + L++EA   AKLA+T    +LI +F   + LK +      + H 
Sbjct: 252 ALIETWRRGGSSIRQR-LDLEARAVAKLAQTPTCRNLIRVFFLQERLKAQGAG---VEHG 307

Query: 314 VKQAAVLGAGIMGGGIAYQSAVKGTPILMKDIREEAIQLGLNEASKLLGNRVEKGRLTPA 373
           + +  V+GAG+MGG IA  +A++G  + ++D  +  ++  +     L   ++      P 
Sbjct: 308 IVRIHVVGAGVMGGDIAAWAALRGFEVTLQDREDAYVRPAIERGRALFAKKLR----APE 363

Query: 374 KMAEALNAIRPTLSYGDFANVDIVVEAVVENPKVKQAVLAEVEGQVKDDAILASNTSTIS 433
           ++  A   +   +     A  D+V+EA+ EN + KQA+  ++E ++K  A+LASNTS+I 
Sbjct: 364 RVEAASRRLVADVEGAGVAEADLVIEAIFENLEAKQALYRQLEPRMKPGALLASNTSSIP 423

Query: 434 INLLAKALKRPENFVGMHFFNPVHMMPLVEVIRGEKSSDVAVATTVAYAKKMGKNPIVVN 493
           ++ L  A+  P  F+G+H+FNPV +MPLVE++R ++    A     A  K + K PI V 
Sbjct: 424 LDELRAAVATPGRFLGLHYFNPVALMPLVEIVRHDRLDPAAEKRAAALCKALDKLPIAVA 483

Query: 494 DCPGFLVNRVLFPYFGGFAKLVSAGVDFVRIDKVMEKFGWPMGPAYLMDVVGID----TG 549
             PGFLVNR+L PY     +++  GV    IDKV  KFG PMGP  L D +G+D     G
Sbjct: 484 GTPGFLVNRILMPYLLEAIRILKEGVPAPVIDKVARKFGMPMGPIELADTIGLDVCASVG 543

Query: 550 HHGRDVMAEGFPDRMKDERRSAVDALYEANRLGQKNGKGFYAYETDKRGKPKKVFDATVL 609
                 +    P+         +D   EA   G+K G+GFY +E  K  KP+        
Sbjct: 544 RELAPFLGLDLPE--------GIDTQLEAGHRGKKTGQGFYRWENGKPVKPE-------- 587

Query: 610 DVLKPIVFEQREVTDEDIINWMMVPLCLETVRCLEDGIVETAAEADMGLVYGIGFPPFRG 669
                   + + V   D+ + +++PL  E V CL D +V+ A   D G+++G GF PFRG
Sbjct: 588 -------VDPKYVAPADLEDRLILPLLNEAVACLHDRVVDDADLLDAGVIFGTGFAPFRG 640

Query: 670 GALRYIDSIGVAEFVA 685
           G + +I ++G A   A
Sbjct: 641 GPIAHIRAVGAATLEA 656


Lambda     K      H
   0.319    0.137    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1183
Number of extensions: 47
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 715
Length of database: 682
Length adjustment: 39
Effective length of query: 676
Effective length of database: 643
Effective search space:   434668
Effective search space used:   434668
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources