GapMind for catabolism of small carbon sources

 

Finding step potA for putrescine catabolism in Nocardioides dokdonensis FR1436

5 candidates for potA: putrescine ABC transporter, ATPase component (PotA/PotG)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi I601_RS08935 ABC transporter ATP-binding protein PotG aka B0855, component of Putrescine porter (characterized) 42% 100% 288.9 Uncharacterized ABC transporter ATP-binding protein YdcT 45% 271.2
hi I601_RS12740 ABC transporter ATP-binding protein PotG aka B0855, component of Putrescine porter (characterized) 46% 93% 280.8 CP4-6 prophage; ABC transporter ATP-binding protein AfuC 42% 260.4
med I601_RS08120 ABC transporter ATP-binding protein spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized) 40% 86% 225.7 FutC aka SLL1878, component of Ferric iron (Fe3+) porter 44% 265.8
med I601_RS08200 ABC transporter ATP-binding protein PotG aka B0855, component of Putrescine porter (characterized) 44% 80% 224.9 FutC aka SLL1878, component of Ferric iron (Fe3+) porter 37% 204.1
lo I601_RS10130 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized) 48% 62% 231.9 Trehalose import ATP-binding protein SugC; EC 7.5.2.- 54% 389.8

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams). For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001.

Definition of step potA

Or cluster all characterized potA proteins

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory