Align aspartate ammonia-lyase (EC 4.3.1.1) (characterized)
to candidate WP_008814805.1 M988_RS18285 aspartate ammonia-lyase
Query= BRENDA::P0AC38 (478 letters) >NCBI__GCF_001655005.1:WP_008814805.1 Length = 478 Score = 885 bits (2287), Expect = 0.0 Identities = 438/478 (91%), Positives = 464/478 (97%) Query: 1 MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDIPEFVRGMVMVKKAAAM 60 MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDIPEFVRGMVMVKKAAA+ Sbjct: 1 MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDIPEFVRGMVMVKKAAAL 60 Query: 61 ANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGAGTSVNMNTNEVLANIGLE 120 ANKEL TIP+ +A+ II ACDEVLNNGKCMDQFPVDVYQGGAGTSVNMNTNEVLANIGLE Sbjct: 61 ANKELHTIPRKIADTIIQACDEVLNNGKCMDQFPVDVYQGGAGTSVNMNTNEVLANIGLE 120 Query: 121 LMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSLIKLVDAINQLREGFERKAVE 180 LMGHQKGEYQYLNPNDH+NKCQSTNDAYPTGFRIAVYSS++KL+DAINQLREGF+RKA E Sbjct: 121 LMGHQKGEYQYLNPNDHLNKCQSTNDAYPTGFRIAVYSSIMKLLDAINQLREGFDRKAKE 180 Query: 181 FQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNIQRTAELLLEVNLGATAIGTGLN 240 F++ILKMGRTQLQDAVPMTLGQEF+A+S+LLKEEVKN+QRT+ELLLEVNLGATAIGTGLN Sbjct: 181 FENILKMGRTQLQDAVPMTLGQEFKAYSVLLKEEVKNLQRTSELLLEVNLGATAIGTGLN 240 Query: 241 TPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGAYVMVHGALKRLAVKMSKICNDLRL 300 TP+ YS LAV+KL+EV+G PC AEDLIEATSDCGAYVMVHGALKRLAVK+SKICNDLRL Sbjct: 241 TPEGYSQLAVQKLSEVSGLPCSVAEDLIEATSDCGAYVMVHGALKRLAVKLSKICNDLRL 300 Query: 301 LSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTMAAEAGQLQ 360 LSSGPR GLNEINLPELQAGSSIMPAKVNPVVPEVVNQ+CFKVIGNDTTVTMAAEAGQLQ Sbjct: 301 LSSGPRTGLNEINLPELQAGSSIMPAKVNPVVPEVVNQLCFKVIGNDTTVTMAAEAGQLQ 360 Query: 361 LNVMEPVIGQAMFESVHILTNACYNLLEKCINGITANKEVCEGYVYNSIGIVTYLNPFIG 420 LNVMEPVIGQAMFES+ ILTN CYNLLEKCINGITANKEVCE YV+NSIGIVTYLNPFIG Sbjct: 361 LNVMEPVIGQAMFESISILTNGCYNLLEKCINGITANKEVCESYVFNSIGIVTYLNPFIG 420 Query: 421 HHNGDIVGKICAETGKSVREVVLERGLLTEAELDDIFSVQNLMHPAYKAKRYTDESEQ 478 HHNGDIVGKICAETGK+VREVVLERGLLTEAELDDIFSV+NLMHPAYKAKRYTDESEQ Sbjct: 421 HHNGDIVGKICAETGKNVREVVLERGLLTEAELDDIFSVENLMHPAYKAKRYTDESEQ 478 Lambda K H 0.317 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 802 Number of extensions: 16 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 478 Length of database: 478 Length adjustment: 34 Effective length of query: 444 Effective length of database: 444 Effective search space: 197136 Effective search space used: 197136 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_008814805.1 M988_RS18285 (aspartate ammonia-lyase)
to HMM TIGR00839 (aspA: aspartate ammonia-lyase (EC 4.3.1.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00839.hmm # target sequence database: /tmp/gapView.172254.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00839 [M=468] Accession: TIGR00839 Description: aspA: aspartate ammonia-lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-272 890.1 3.5 2e-272 889.9 3.5 1.0 1 NCBI__GCF_001655005.1:WP_008814805.1 Domain annotation for each sequence (and alignments): >> NCBI__GCF_001655005.1:WP_008814805.1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 889.9 3.5 2e-272 2e-272 1 468 [] 6 474 .. 6 474 .. 1.00 Alignments for each domain: == domain 1 score: 889.9 bits; conditional E-value: 2e-272 TIGR00839 1 riekdllGekeveaevyyGiqtlrasenfaisnekisdvpefvkalvlvkkaaalanvelkaidekiakaiva 73 rie+dllG++ev+a++yyG++tlra+enf+isn+kisd+pefv+++v+vkkaaalan+el++i++kia +i++ NCBI__GCF_001655005.1:WP_008814805.1 6 RIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDIPEFVRGMVMVKKAAALANKELHTIPRKIADTIIQ 78 89*********************************************************************** PP TIGR00839 74 acdeile.GkyldqfivdviqGGaGtsvnmntnevianlalellGhkkGeyqflnpndhvnksqstndaypta 145 acde+l+ Gk++dqf+vdv+qGGaGtsvnmntnev+an++lel+Gh+kGeyq+lnpndh+nk+qstndaypt+ NCBI__GCF_001655005.1:WP_008814805.1 79 ACDEVLNnGKCMDQFPVDVYQGGAGTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHLNKCQSTNDAYPTG 151 ******99***************************************************************** PP TIGR00839 146 lkiavyesleklvdkiealrdafeqkakefadvlkmGrtqlqdavpltlGqefeayalllerdvkrikrtrel 218 ++iavy+s++kl+d+i++lr++f++kakef+++lkmGrtqlqdavp+tlGqef+ay++ll+++vk+++rt el NCBI__GCF_001655005.1:WP_008814805.1 152 FRIAVYSSIMKLLDAINQLREGFDRKAKEFENILKMGRTQLQDAVPMTLGQEFKAYSVLLKEEVKNLQRTSEL 224 ************************************************************************* PP TIGR00839 219 llevnlGataiGtGlnadkeysklvvkklaevtGlplvpaenlieatsdtgayvevsgalkriavklskvcnd 291 llevnlGataiGtGln++++ys+l+v+kl+ev+Glp+ ae+lieatsd+gayv+v+galkr+avklsk+cnd NCBI__GCF_001655005.1:WP_008814805.1 225 LLEVNLGATAIGTGLNTPEGYSQLAVQKLSEVSGLPCSVAEDLIEATSDCGAYVMVHGALKRLAVKLSKICND 297 ************************************************************************* PP TIGR00839 292 lrllssGpraGlneinlpelqaGssimpakvnpvvpevvnqvcfkviGndttvtlaaeaGqlqlnvlepviaf 364 lrllssGpr+GlneinlpelqaGssimpakvnpvvpevvnq+cfkviGndttvt+aaeaGqlqlnv+epvi++ NCBI__GCF_001655005.1:WP_008814805.1 298 LRLLSSGPRTGLNEINLPELQAGSSIMPAKVNPVVPEVVNQLCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQ 370 ************************************************************************* PP TIGR00839 365 allesisiltnaiesltdkcveGitanekicedyvfnsiGivtalnpfiGyekaalvakeaiktgksvrdvvl 437 a++esisiltn++++l++kc++Gitan+++ce+yvfnsiGivt+lnpfiG++++++v+k++++tgk vr+vvl NCBI__GCF_001655005.1:WP_008814805.1 371 AMFESISILTNGCYNLLEKCINGITANKEVCESYVFNSIGIVTYLNPFIGHHNGDIVGKICAETGKNVREVVL 443 ************************************************************************* PP TIGR00839 438 ekdllteeelddilsvenllkpaykakklkd 468 e++llte+elddi+svenl++paykak+++d NCBI__GCF_001655005.1:WP_008814805.1 444 ERGLLTEAELDDIFSVENLMHPAYKAKRYTD 474 ****************************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (468 nodes) Target sequences: 1 (478 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 16.38 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory