Align GluC aka CGL1952, component of Glutamate porter (characterized)
to candidate WP_067473904.1 BJL86_RS10995 amino acid ABC transporter permease
Query= TCDB::P48244 (228 letters) >NCBI__GCF_001659785.1:WP_067473904.1 Length = 224 Score = 300 bits (767), Expect = 2e-86 Identities = 155/225 (68%), Positives = 185/225 (82%), Gaps = 1/225 (0%) Query: 4 LWADLGPSLLPAFWVTIKLTIYSAIGAMIFGTILTTMRVSPVKILRTLSTAYINTVRNTP 63 +W + GP+L PAFW+TIKLT +SAIG++I+GTILT +RV PV ILR S AY+N VRNTP Sbjct: 1 MWEEFGPALWPAFWMTIKLTFFSAIGSLIWGTILTALRVCPVPILRGFSAAYVNIVRNTP 60 Query: 64 LTLVVLFCSFGLYQNLGLTLAGRESSTFLVDNNFRLAVLGFILYTSTFVAESLRSGINTV 123 LTLVVLFCS GLYQNLGL LA E+ F+ +NNF LAVL FILYTSTFV E+LRSGINTV Sbjct: 61 LTLVVLFCSMGLYQNLGLALAP-ENDHFIENNNFNLAVLAFILYTSTFVCETLRSGINTV 119 Query: 124 HFGQAEAARSLGLGFGATFRSIIFPQAVRAAIVPLGNTLIALTKNTTIASVIGVGEASLL 183 GQAEAARSLGLGFG I+ PQA R+ I PL + LIAL KNTTIASVIGV EASLL Sbjct: 120 PMGQAEAARSLGLGFGQILGLIVLPQAFRSVINPLASVLIALIKNTTIASVIGVAEASLL 179 Query: 184 MKATIENHANMLFVVFAIFAVGFMILTLPMGLGLGKLSERLAVKK 228 MK+TIE++A L+V+FA+ A+GF+ILTLP+GL LGK+SE++AVK+ Sbjct: 180 MKSTIESYAGDLYVIFAVIALGFVILTLPVGLLLGKVSEKVAVKR 224 Lambda K H 0.327 0.139 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 260 Number of extensions: 9 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 228 Length of database: 224 Length adjustment: 22 Effective length of query: 206 Effective length of database: 202 Effective search space: 41612 Effective search space used: 41612 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory