Alignments for a candidate for bkdC in Chryseobacterium arthrosphaerae CC-VM-7

GapMind for catabolism of small carbon sources

Alignments for a candidate for bkdC in Chryseobacterium arthrosphaerae CC-VM-7

Align Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; EC 2.3.1.168; Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (uncharacterized)
to candidate WP_065397619.1 BBI00_RS04345 pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Query= curated2:P37942
         (424 letters)



>NCBI__GCF_001684965.1:WP_065397619.1
          Length = 532

 Score =  235 bits (600), Expect = 2e-66
 Identities = 146/424 (34%), Positives = 230/424 (54%), Gaps = 29/424 (6%)

Query: 3   IEQMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKVNAEVPSSFTGTITELVG 62
           +E +TMP+L +++TEG ++KW    GD V + D +AE+ TDK   +  S F G + +   
Sbjct: 122 VEVITMPRLSDTMTEGKVAKWHKNVGDTVKEGDLLAEIETDKAVQDFESEFNGVLLKQGV 181

Query: 63  EEGQTLQVGEMICKIETEGAN--------PAEQKQEQPAASEAAENPVAKSAGAADQPNK 114
           EEG    V  ++  I  EG +         A    E+PA  +A      K+A AA+  + 
Sbjct: 182 EEGGAAPVDSVLAIIGPEGTDVSAVGAPKAAAPASEKPAEQKAEAKTEEKAAPAANTSSS 241

Query: 115 KRY--SPAVLRLAGEHGIDLDQVTGTGAGGRITRKDIQRLIETGGVQEQNPEELKTAAPA 172
            R   SP   ++A + G+D++ V G+G  GRI +KDI+          Q   +   +APA
Sbjct: 242 DRVAISPLAKKMAQDKGVDINAVQGSGENGRIVKKDIENY--------QPAAQPAASAPA 293

Query: 173 PKSASKPEPKEETSYPASAAGDKEIPVTGVRKAIASNMKRSKTEIPHAWTMMEVDVTNMV 232
             +A+      + +       D E P + VR  IA  +  SK   PH + M+E+++   +
Sbjct: 294 ASAAA------QVAVNFVQGEDTETPNSQVRNIIAKRLSESKFSAPHYYLMVEINMDKAI 347

Query: 233 AYRNSIKDSFKKTEGFNLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQKKDINISIAVA 292
             R  I +S   T+   ++F    +KA A AL++ PQ+NS WAGDKII + +INI +AVA
Sbjct: 348 EARKEI-NSLPDTK---ISFNDMIIKATAIALRKHPQVNSSWAGDKIIHRGNINIGVAVA 403

Query: 293 TEDSLFVPVIKNADEKTIKGIAKDITGLAKKVRDGKLTADDMQGGTFTVNNTGSFGSVQS 352
             D L VPV+KN D+ +   I+  +  +A + ++  L A++M+G TF+++N G FG    
Sbjct: 404 IPDGLVVPVLKNTDQMSYTQISAAVKDMASRAKNKGLKANEMEGSTFSISNLGMFGIETF 463

Query: 353 MGIINYPQAAILQVESIVKRPVVMDNGMIAVRDMVNLCLSLDHRVLDGLVCGRFLGRVKQ 412
             IIN P +AIL V +I+++P+V D G I V + + L L+ DHRV+DG    +FL  ++ 
Sbjct: 464 TSIINQPNSAILSVGAIIEKPIVKD-GQIVVGNTMKLSLACDHRVVDGATGAQFLQTLRT 522

Query: 413 ILES 416
            LES
Sbjct: 523 YLES 526



 Score = 62.0 bits (149), Expect = 4e-14
 Identities = 38/115 (33%), Positives = 57/115 (49%), Gaps = 14/115 (12%)

Query: 4   EQMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKVNAEVPSSFTGTITELVGE 63
           E +TMP+L +++TEG ++KW    GDKV + D +AE+ TDK   +  S   GT+  +  E
Sbjct: 3   EVITMPRLSDTMTEGKVAKWHKKVGDKVKEGDILAEIETDKAVQDFESEVEGTLLYVGVE 62

Query: 64  EGQTLQVGEMICKIETEG--------------ANPAEQKQEQPAASEAAENPVAK 104
           EG    V  ++  I  EG              A   E+K E+   +EA E   A+
Sbjct: 63  EGSAAAVDSVLAIIGNEGEDISGLTGGAAAPSAGSEEKKSEEQPKTEAVETASAE 117


Lambda     K      H
   0.312    0.129    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 584
Number of extensions: 27
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 424
Length of database: 532
Length adjustment: 33
Effective length of query: 391
Effective length of database: 499
Effective search space:   195109
Effective search space used:   195109
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources