GapMind for catabolism of small carbon sources

 

Alignments for a candidate for natA in Chryseobacterium arthrosphaerae CC-VM-7

Align NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized)
to candidate WP_065398591.1 BBI00_RS09790 ATP-binding cassette domain-containing protein

Query= TCDB::Q7A2H0
         (260 letters)



>NCBI__GCF_001684965.1:WP_065398591.1
          Length = 342

 Score =  122 bits (307), Expect = 8e-33
 Identities = 75/233 (32%), Positives = 131/233 (56%), Gaps = 23/233 (9%)

Query: 25  KAVQEARIEVAQGSITGLIGPNGAGKTTLFNLLSNFIRPDKGRVIFDGEPIQQLQPHQIA 84
           KA+ +  + + +G I G+IG +GAGK+TL   ++   RPD+G++I +G+   +L   Q+A
Sbjct: 19  KALDKVSLNIEKGDIVGIIGFSGAGKSTLIRTVNLLERPDEGQIIINGKDFTKLNSRQLA 78

Query: 85  QQ----GMVRTFQVARTLSRLSVLENMLLAAQ-KQTGENFWQVQLQPQVVVKEEKQLQEQ 139
           ++    GM+  FQ    LS  +V +N+ L  +   TG+                +Q+  +
Sbjct: 79  EERKKIGMI--FQHFNLLSSRTVFDNVALPLELDHTGK----------------EQINRK 120

Query: 140 AMFLLESVGLAKKAYEYAGGLSGGQRKLLEMGRALMTNPKLILLDEPAAGVNPRLIDDIC 199
              LL+ VGL +KA +Y   LSGGQ++ + + RAL  +P L+L DE  + ++P     I 
Sbjct: 121 VNELLKIVGLEEKANDYPRSLSGGQKQRVAIARALANDPHLLLCDEATSALDPATTQSIL 180

Query: 200 DRILTWNRQDGMTFLIIEHNMDVIMSLCDRVWVLAEGQNLADGTPAEIQTNSQ 252
             +   N++ G+T L+I H M+VI ++C+ V V+ +G+ L  GT +EI ++ +
Sbjct: 181 QLLRDINQRLGITILLITHEMEVIKTVCNHVAVIDKGKLLTKGTLSEIISDKE 233


Lambda     K      H
   0.319    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 165
Number of extensions: 5
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 342
Length adjustment: 26
Effective length of query: 234
Effective length of database: 316
Effective search space:    73944
Effective search space used:    73944
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory