Align AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized)
to candidate WP_069958139.1 BEN30_RS09690 ATP-binding cassette domain-containing protein
Query= TCDB::Q52815 (257 letters) >NCBI__GCF_001746755.1:WP_069958139.1 Length = 259 Score = 218 bits (556), Expect = 8e-62 Identities = 114/251 (45%), Positives = 161/251 (64%), Gaps = 11/251 (4%) Query: 18 VEIVNMNKWYGDFHVLRDINLKVMRGERIVIAGPSGSGKSTMIRCINRLEEHQKGKIVVD 77 +++ +++K +G VL+ I++ RG+ I I G SGSGKST +RCIN LE G++ + Sbjct: 9 LKVEDIHKSFGGQDVLKGISVNANRGDVISILGSSGSGKSTFLRCINYLEHPDIGRVSIA 68 Query: 78 GTELTN-----------DLKKIDEVRREVGMVFQHFNLFPHLTILENCTLAPIWVRKMPK 126 G E+ D K++ +R +G VFQ FNL+PHL +L+N AP+ V K+ + Sbjct: 69 GEEIRTKKDKSGSLVPADPKQLARLRARIGFVFQSFNLWPHLNVLQNVIEAPMQVLKLSR 128 Query: 127 KQAEEVAMHFLKRVKIPEQANKYPGQLSGGQQQRVAIARSLCMNPKIMLFDEPTSALDPE 186 K A E A L RV + ++ + +P LSGGQQQR AIAR+L M P+++LFDEPTSALDPE Sbjct: 129 KDATERAEVILARVGLADRMHHFPAHLSGGQQQRAAIARTLAMEPEVILFDEPTSALDPE 188 Query: 187 MIKEVLDTMVGLAEEGMTMLCVTHEMGFARQVANRVIFMDQGQIVEQNEPAAFFDNPQHE 246 ++ EVL + LAEEG TML VTHEMGFAR+V+ +F+ QG++ EQ P F N E Sbjct: 189 LVSEVLAVIGSLAEEGRTMLIVTHEMGFAREVSTWTMFLHQGKVEEQGPPDKIFKNADSE 248 Query: 247 RTKLFLSQILH 257 R + F++ LH Sbjct: 249 RVRQFMASHLH 259 Lambda K H 0.321 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 169 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 257 Length of database: 259 Length adjustment: 24 Effective length of query: 233 Effective length of database: 235 Effective search space: 54755 Effective search space used: 54755 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory