GapMind for catabolism of small carbon sources

 

Alignments for a candidate for aapP in Magnetovibrio blakemorei MV-1

Align AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized)
to candidate WP_069958139.1 BEN30_RS09690 ATP-binding cassette domain-containing protein

Query= TCDB::Q52815
         (257 letters)



>NCBI__GCF_001746755.1:WP_069958139.1
          Length = 259

 Score =  218 bits (556), Expect = 8e-62
 Identities = 114/251 (45%), Positives = 161/251 (64%), Gaps = 11/251 (4%)

Query: 18  VEIVNMNKWYGDFHVLRDINLKVMRGERIVIAGPSGSGKSTMIRCINRLEEHQKGKIVVD 77
           +++ +++K +G   VL+ I++   RG+ I I G SGSGKST +RCIN LE    G++ + 
Sbjct: 9   LKVEDIHKSFGGQDVLKGISVNANRGDVISILGSSGSGKSTFLRCINYLEHPDIGRVSIA 68

Query: 78  GTELTN-----------DLKKIDEVRREVGMVFQHFNLFPHLTILENCTLAPIWVRKMPK 126
           G E+             D K++  +R  +G VFQ FNL+PHL +L+N   AP+ V K+ +
Sbjct: 69  GEEIRTKKDKSGSLVPADPKQLARLRARIGFVFQSFNLWPHLNVLQNVIEAPMQVLKLSR 128

Query: 127 KQAEEVAMHFLKRVKIPEQANKYPGQLSGGQQQRVAIARSLCMNPKIMLFDEPTSALDPE 186
           K A E A   L RV + ++ + +P  LSGGQQQR AIAR+L M P+++LFDEPTSALDPE
Sbjct: 129 KDATERAEVILARVGLADRMHHFPAHLSGGQQQRAAIARTLAMEPEVILFDEPTSALDPE 188

Query: 187 MIKEVLDTMVGLAEEGMTMLCVTHEMGFARQVANRVIFMDQGQIVEQNEPAAFFDNPQHE 246
           ++ EVL  +  LAEEG TML VTHEMGFAR+V+   +F+ QG++ EQ  P   F N   E
Sbjct: 189 LVSEVLAVIGSLAEEGRTMLIVTHEMGFAREVSTWTMFLHQGKVEEQGPPDKIFKNADSE 248

Query: 247 RTKLFLSQILH 257
           R + F++  LH
Sbjct: 249 RVRQFMASHLH 259


Lambda     K      H
   0.321    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 169
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 257
Length of database: 259
Length adjustment: 24
Effective length of query: 233
Effective length of database: 235
Effective search space:    54755
Effective search space used:    54755
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory