GapMind for catabolism of small carbon sources

 

Protein WP_047213152.1 in Pandoraea thiooxydans ATSB16

Annotation: NCBI__GCF_001931675.1:WP_047213152.1

Length: 458 amino acids

Source: GCF_001931675.1 in NCBI

Candidate for 16 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-phenylalanine catabolism aroP hi Aromatic amino acid transport protein AroP (characterized, see rationale) 83% 96% 759.2 L-tyrosine transporter 63% 574.3
L-tryptophan catabolism aroP hi Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan (characterized) 71% 97% 648.3 Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs 63% 583.6
L-tyrosine catabolism aroP hi Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan (characterized) 71% 97% 648.3 Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs 63% 583.6
phenylacetate catabolism H281DRAFT_04042 med Aromatic amino acid transporter AroP (characterized, see rationale) 68% 98% 630.2 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-threonine catabolism RR42_RS28305 med D-serine/D-alanine/glycine transporter (characterized, see rationale) 43% 96% 421.8 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
D-alanine catabolism cycA med L-alanine and D-alanine permease (characterized) 45% 98% 406.4 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-alanine catabolism cycA med L-alanine and D-alanine permease (characterized) 45% 98% 406.4 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-proline catabolism proY med Proline-specific permease (ProY) (characterized) 46% 98% 393.7 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-histidine catabolism permease med histidine permease (characterized) 43% 97% 386.3 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
D-serine catabolism cycA med D-serine/D-alanine/glycine transporter (characterized) 42% 97% 360.5 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-asparagine catabolism ansP lo Asparagine permease (AnsP) of 497 aas and 12 TMSs (characterized) 38% 90% 332 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-arginine catabolism rocE lo Amino-acid permease RocE (characterized) 34% 97% 292.4 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-serine catabolism serP lo Serine uptake transporter, SerP1, of 259 aas and 12 TMSs (Trip et al. 2013). L-serine is the highest affinity substrate (Km = 18 μM), but SerP1 also transports L-threonine and L-cysteine (Km values = 20 - 40 μM) (characterized) 35% 98% 289.7 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-threonine catabolism serP1 lo Serine uptake transporter, SerP1, of 259 aas and 12 TMSs (Trip et al. 2013). L-serine is the highest affinity substrate (Km = 18 μM), but SerP1 also transports L-threonine and L-cysteine (Km values = 20 - 40 μM) (characterized) 35% 98% 289.7 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-lysine catabolism lysP lo Lysine/arginine permease CAN1; Basic amino acids permease CAN1 (characterized) 32% 82% 245.7 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3
L-tryptophan catabolism TAT lo tryptophan permease (characterized) 33% 66% 206.8 Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan 71% 648.3

Sequence Analysis Tools

View WP_047213152.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MVSHNAEDGLKRGLKNRHIQLIALGGAIGTGLFLGIAQTIKMAGPSVLLGYAIGGIIAFF
IMRQLGELVVDEPVAGSFSYFANRYLGSFAGFTSGWNYWVLYILVSMAELSAVGIYIQYW
WPQIPTWVSALGFFVIINVLNLTSVKSYGETEFWFAIIKVAAIIGMILFGGYLLFSGKAG
PDASVANLWQHGGFFPHGVSGLVMAMAVIMFSFGGLELVGITAAEADDPSHSIPRATNQV
IYRILIFYIGALSVLLSLYPWEKVVTGGSPFVLIFHAMDSNLVANALNVVVLTAALSVYN
SCVYCTSRMLFGLAQQGNAPQALLKVNRRGIPLAALGVSSLVTGLCVLINYLMPGKAFEM
LMGLVVSALLINWAMISIIHLKFRHDKRRAGLQTRFKSLGYPLTNYLCLAFLAGILVIMY
LTPELRISVYLIPLWLVVLAVSYRFKRPGAALRGASAR

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory