GapMind for catabolism of small carbon sources

 

Protein WP_047215662.1 in Pandoraea thiooxydans ATSB16

Annotation: NCBI__GCF_001931675.1:WP_047215662.1

Length: 504 amino acids

Source: GCF_001931675.1 in NCBI

Candidate for 11 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-lysine catabolism amaB hi L-piperidine-6-carboxylate dehydrogenase; EC 1.2.1.21 (characterized, see rationale) 69% 100% 686.8 Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Matured fruit 60 kDa protein; MF-60; EC 1.2.1.3 48% 472.6
4-hydroxybenzoate catabolism adh med Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Matured fruit 60 kDa protein; MF-60; EC 1.2.1.3 (characterized) 48% 94% 472.6 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4
2'-deoxyinosine catabolism adh med Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Matured fruit 60 kDa protein; MF-60; EC 1.2.1.3 (characterized) 48% 94% 472.6 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4
2-deoxy-D-ribose catabolism adh med Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Matured fruit 60 kDa protein; MF-60; EC 1.2.1.3 (characterized) 48% 94% 472.6 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4
ethanol catabolism adh med Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Matured fruit 60 kDa protein; MF-60; EC 1.2.1.3 (characterized) 48% 94% 472.6 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4
L-threonine catabolism adh med Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Matured fruit 60 kDa protein; MF-60; EC 1.2.1.3 (characterized) 48% 94% 472.6 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4
thymidine catabolism adh med Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Matured fruit 60 kDa protein; MF-60; EC 1.2.1.3 (characterized) 48% 94% 472.6 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4
L-tryptophan catabolism adh med Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Matured fruit 60 kDa protein; MF-60; EC 1.2.1.3 (characterized) 48% 94% 472.6 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4
4-hydroxybenzoate catabolism boxD lo 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase; EC 1.2.1.77 (characterized) 30% 57% 107.1 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4
phenylacetate catabolism boxD lo 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase; EC 1.2.1.77 (characterized) 30% 57% 107.1 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4
L-phenylalanine catabolism boxD lo 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase; EC 1.2.1.77 (characterized) 30% 57% 107.1 Δ1-piperideine-6-carboxylate dehydrogenase 57% 483.4

Sequence Analysis Tools

View WP_047215662.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MNPAALLQSLGVDIAALEGSDLPVRSPRDGARLAVLRADDAQSVERKIALAHAAFERWCV
VPAPVRGELVRLLGQVLRERKTELGQLVTLEAGKILSEGLGEVQEMIDICDFAVGLSRQL
HGLTIASERPGHRMMETWHPLGVCGVISAFNFPVAVWSWNSALAFVCGDAVVWKPSEKTP
LTALACEALLRQAIARFEAARPGVVPTGLLQVVLGGAEAGDVLAASTRVPVVSATGSTRM
GRAVAQRVAQRLGRSILELGGNNAMIVTPSAELDLALRAITFAAVGTAGQRCTSLRRLIV
HRSLTDTLCERLKKIYGSVKIGDPLASDTLVGPLIDRAAFEQMQQALAQAREQGGEVFGG
ERVASDLGDDAYYVRPALVRMPAQTDVMRHETFAPILYVLSYDDYAQALQLHNDVPQGLS
SAVFTNDMREAERFMSPQGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKGY
MRRATNTINFSTELPLAQGVRFDI

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory