Alignments for a candidate for nagF in Pandoraea thiooxydans ATSB16

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Pandoraea thiooxydans ATSB16

Align N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized)
to candidate WP_047215151.1 PATSB16_RS16515 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::pseudo5_N2C3_1:AO356_17540
         (837 letters)



>NCBI__GCF_001931675.1:WP_047215151.1
          Length = 578

 Score =  298 bits (764), Expect = 5e-85
 Identities = 196/542 (36%), Positives = 283/542 (52%), Gaps = 19/542 (3%)

Query: 278 LHGVCAAPGLVTGPLVRLSGIQLPED---IGGHAIEEQRQRLSDALAQVRGEIHLTLEHA 334
           LHG+  + G+  G    L+   L      I    +E +  R  +A   V+ E+       
Sbjct: 5   LHGIPVSQGIAIGRAYLLAAATLDVPHYLIEPSQVESEIARFYEARRAVQQELDTLKAEL 64

Query: 335 RARQHRDEEAIFSAHLALLEDPVLLDAA-DLFIEQGSAAPHAWSRSIDTQCQVLQQLGST 393
            A    +  A    H  ++ D +L D A DL       A  A    ++      + +   
Sbjct: 65  PADAPGEMSAFLDVHSLIVNDAMLADTALDLIRSSHYNAEWALMTQVEALIARFEDIEDE 124

Query: 394 LLAERANDLRDLRQRVLRVLLGEAWQFDVAAGA-----IVAAQELTPSDLLQLSAQGVAG 448
            L ER  D+  +  R+L+VL G      VA  A     IV A+++ P+D++Q   Q   G
Sbjct: 125 YLRERKADIEQVADRMLKVLAGVPGIRSVAVQAPRDDMIVVARDIAPADMMQFKTQVFRG 184

Query: 449 VCMVEGGATSHVAILARGKGLPCLVALGDELLAQEQGQAVVLDADGGRLELTPTVERLAQ 508
                GG TSH AI+AR  G+P  V +       EQG  +++D D G + + P    L +
Sbjct: 185 FVTDLGGKTSHTAIVARSLGIPAAVGVQHASQLIEQGDLIIIDGDQGVVIIDPAPMILEE 244

Query: 509 VRQAQTRRTALRAQQQSLAHTPARTVDGVEVEVAANVASSAEAGESLANGADGVGLLRTE 568
            R  Q+ +   + + Q L ++PA+T+DG  V + AN+    +A  ++A GA GVGL R+E
Sbjct: 245 YRYRQSEKVLEQRRLQRLRYSPAQTIDGTPVALLANIELPEDAEAAVAAGAVGVGLFRSE 304

Query: 569 FLFVDRHTAPDEEEQRQAYQAVLEAMGDKPVIIRTIDVGGDKQLD-YLPLPSEANPVLGL 627
           FLF+++   PDEE Q +AY+  +  M   PV IRTIDVG DK LD +    + ANP +GL
Sbjct: 305 FLFMNQADMPDEEAQFEAYRRAVATMQGLPVTIRTIDVGADKSLDGHESYENGANPAMGL 364

Query: 628 RGIRLAQVRPDLLDQQLRALLQTRPLDRCRILLPMVT---EVDELLHI----RKRLDALG 680
           R IR +   P +   QLRALL+   L   RIL PM+    E+D+ L +    +++ DA G
Sbjct: 365 RAIRWSLSEPRMFLTQLRALLRASALGPVRILFPMLAHAQEIDQTLELVQEAKRQCDAAG 424

Query: 681 SELGLSERPQLGVMVEVPAAALLAEQLAEHADFLSIGTNDLSQYTLAMDRDHAGLAARVD 740
             L      +LG MVEVPAAAL      +  DFLSIGTNDL QYTLA+DR    +A   D
Sbjct: 425 --LAYDPNVKLGAMVEVPAAALALPMFLKRLDFLSIGTNDLIQYTLAIDRADNAVAHLYD 482

Query: 741 ALHPALLRLIAQTCAGAAKHGRWVGVCGALASDPLATPVLIGLGVRELSVSPPQIGEIKD 800
            +HPA+L+LIA+T   A   G  + VCG +A DP+ T +L+G+G+RE S+ P QI ++K 
Sbjct: 483 PMHPAVLQLIARTIREARAAGVPIAVCGEMAGDPMLTRLLLGMGLREFSMHPSQILQVKQ 542

Query: 801 RV 802
            V
Sbjct: 543 EV 544


Lambda     K      H
   0.319    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 900
Number of extensions: 38
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 837
Length of database: 578
Length adjustment: 39
Effective length of query: 798
Effective length of database: 539
Effective search space:   430122
Effective search space used:   430122
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources