Alignments for a candidate for acs in Pandoraea thiooxydans ATSB16

GapMind for catabolism of small carbon sources

Alignments for a candidate for acs in Pandoraea thiooxydans ATSB16

Align Acetate/butyrate--CoA ligase AAE7, peroxisomal; AMP-binding protein 7; AtAMPBP7; Acetyl-CoA synthetase; Acyl-activating enzyme 7; Butyryl-CoA synthetase; Protein ACETATE NON-UTILIZING 1; EC 6.2.1.1; EC 6.2.1.2 (characterized)
to candidate WP_047216123.1 PATSB16_RS01460 acyl-CoA synthetase

Query= SwissProt::Q8VZF1
         (569 letters)



>NCBI__GCF_001931675.1:WP_047216123.1
          Length = 557

 Score =  561 bits (1447), Expect = e-164
 Identities = 281/549 (51%), Positives = 371/549 (67%), Gaps = 20/549 (3%)

Query: 10  DDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRSI 69
           + L +  ANY  LTP+ F+ RAA V+  R +V+HG    TW +TY+R RRLASAL    I
Sbjct: 7   EGLDRREANYVPLTPIEFIARAADVYGDRLAVVHGDVRRTWGETYERARRLASALQRAGI 66

Query: 70  GPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQEF 129
             G TVA++ PNIPAM EAHFGVPM G VLN +N RL+  ++ F+L H ++ +++VD EF
Sbjct: 67  ARGDTVAVLLPNIPAMVEAHFGVPMSGGVLNTLNTRLDVASLLFMLRHGEARLLIVDTEF 126

Query: 130 FTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCA-PESLNRALSKGAIEYEDFLATGDPNY 188
             LA         +A   F +  ++ + D   A P +  R+     I+YE FL  GDP +
Sbjct: 127 GELAR--------RAAIEFPQLRIVSVSDVMHADPHAFARS-----IDYEAFLQEGDPRF 173

Query: 189 PWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPMF 248
            ++ PADEW +IAL YTSGTT  PKGVV HHRGAY+ ALSN L W M   AVYLWTLPMF
Sbjct: 174 SYKLPADEWDAIALNYTSGTTGDPKGVVYHHRGAYLNALSNILEWDMPKHAVYLWTLPMF 233

Query: 249 HCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDTI 308
           HCNGWCFPW++A  +G ++CLR+  A  V+ +I + +VTH+C AP+V +A+ NAP +   
Sbjct: 234 HCNGWCFPWTIAARAGVNVCLRKFDAATVFDLIRREQVTHYCGAPIVQSALANAPAQWRE 293

Query: 309 LPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPET 368
             L H V  M AGAAP PS++ SM + GF + H YGL+E YGP++VCA +  W++L  + 
Sbjct: 294 -GLDHRVSTMVAGAAPSPSMIASMKKIGFDLTHVYGLTEVYGPASVCAKQERWEALDDDE 352

Query: 369 QAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKET 428
           +A+LNARQGVRY     + V+D QT +PVP DG+T GEI+FRGN+ MKGYLKN  A +E 
Sbjct: 353 RARLNARQGVRYHLQSAVAVLDPQTLEPVPPDGETVGEIMFRGNICMKGYLKNERATEEA 412

Query: 429 FAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVAR 488
           F GGWFH+GD+ V+ PD Y+ IKDRSKD+IISGGENISS+E+E+ +Y HPAV  A+VVA 
Sbjct: 413 FRGGWFHTGDLGVQAPDGYVRIKDRSKDIIISGGENISSIEIEDTLYRHPAVSVAAVVAM 472

Query: 489 PDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATGK 548
           PD +W E PCAFV LK   +       A +++  CR  L  Y +PK++  G +PKT+TGK
Sbjct: 473 PDPKWGEVPCAFVELKEGIDAS-----ADELIAHCRLFLAGYKLPKAIHIGEVPKTSTGK 527

Query: 549 IQKHILRTK 557
           IQK  LR +
Sbjct: 528 IQKFELRAR 536


Lambda     K      H
   0.319    0.134    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 876
Number of extensions: 38
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 569
Length of database: 557
Length adjustment: 36
Effective length of query: 533
Effective length of database: 521
Effective search space:   277693
Effective search space used:   277693
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources