Alignments for a candidate for etoh-dh-c in Pandoraea thiooxydans ATSB16

GapMind for catabolism of small carbon sources

Alignments for a candidate for etoh-dh-c in Pandoraea thiooxydans ATSB16

Align quinoprotein decanol dehydrogenase; EC 1.1.-.- (characterized)
to candidate WP_047215054.1 PATSB16_RS15940 PQQ-dependent dehydrogenase, methanol/ethanol family

Query= CharProtDB::CH_107432
         (595 letters)



>NCBI__GCF_001931675.1:WP_047215054.1
          Length = 575

 Score =  432 bits (1110), Expect = e-125
 Identities = 234/581 (40%), Positives = 329/581 (56%), Gaps = 28/581 (4%)

Query: 9   MFAVSLVLSAMLLAGAAHAAVSNEEILQDPKNPQQIVTNGLGVQGQRYSPLDLLNANNVK 68
           + A  L + A     A + AV+ + +     +P  + T      GQ  SPL  +N    K
Sbjct: 18  LLASGLAIGAASAQTADYPAVTYDRLTSAQADPGWL-TYYRSYTGQSNSPLKQINTATAK 76

Query: 69  ELRPVWAFSFGGEKQRGQQAQPLIKDGVMYLTGSYSRVFAVDARTGKKLWQYDARLPDDI 128
           +L+ VW++ F  E Q+G +A P++    M++T     V+A DA+TGK+LW YD +L    
Sbjct: 77  DLQQVWSYKFPAELQQGFEATPIVNGNYMFVTTPKDHVYAFDAKTGKELWTYDPKLTSLS 136

Query: 129 --RPCCDVINRGVALYGDLVFFGTLDAKLVALNKDTGKVVWSKKVADHKEGYSISAAPMI 186
               CCDV+NRGVALYG  V+   L  ++VAL+  +GKVVW K++ +   GY+ + AP+ 
Sbjct: 137 FKTACCDVVNRGVALYGKNVYVAMLSGQVVALDAQSGKVVWEKQLFEPGIGYAFTLAPLA 196

Query: 187 VNGKLITGVAGGEFGVVGKIQAYNPENGELLWMRPTVEGHMGYVYKDGKAIENGISGGEA 246
           +   +I G +GGE+G  G I A NP NGE+ W R TV                     + 
Sbjct: 197 LKDSIIVGSSGGEYGARGFIAALNPANGEIQWKRWTVPA----------------PNEKG 240

Query: 247 GKTWPGDLWKTGGAAPWLGGYYDPETNLILFGTGNPAPWNSHLRPGDNLYSSSRLALNPD 306
           G TWP  +++  G+  WL G YD +TN + +G GNP PW + +RPG NLYS S LAL+P 
Sbjct: 241 GNTWPNGMYQHAGSPAWLTGTYDVKTNTLFWGVGNPGPWLADMRPGKNLYSDSLLALDPA 300

Query: 307 DGTIKWHFQSTPHDGWDFDGVNELISFNYKDGGKEVKAAATADRNGFFYVLDRTNGKFIR 366
           +G +KWHFQ T HD WD+DGVN  +  N    GK  +A   ADRNG+ + +DRTNGK I 
Sbjct: 301 NGDLKWHFQYTSHDTWDYDGVNTPVLANITYKGKHYEAIIHADRNGYIHAIDRTNGKLIY 360

Query: 367 GFPFVDKITWATGLDKDGRPIYNDASRPGAPGSEAKGSSVFVAPAFLGAKNWMPMAYNKD 426
             PFV K T  TG    G PI N    P        G+++   P+FLG KNW  ++Y+  
Sbjct: 361 AKPFV-KATSVTGYTAAGVPIQNQKHYPKV------GTTIETCPSFLGGKNWWSVSYDPT 413

Query: 427 TGLFYVPSNEWGMDIWNEGIAYKKGAAFLGAGFTIKP-LNEDYIGVLRAIDPISGKEVWR 485
           T L +VPS    M +  + + Y +G  +LG GF I+P       G L+AID  +GK+VW 
Sbjct: 414 THLAFVPSLHACMTMSGKAVTYMQGLPYLGEGFEIRPEPGSKGYGELQAIDVNTGKKVWS 473

Query: 486 HKNYAPLWGGVLTTKGNLVFTGTPEGFLQAFNAKTGDKVWEF-QTGSGVLGSPVTWEMDG 544
           H +  P  GGV +T G LVF+G+  G L AF+ K G  +WE  Q  SGV+  P  +E+DG
Sbjct: 474 HWSKLPWNGGVASTAGGLVFSGSLNGHLYAFDQKNGKVLWESPQLASGVIAQPSVFEVDG 533

Query: 545 EQYVSVVSGWGGAVPLWGGEVAKRVKDFNQGGMLWTFKLPK 585
            +YV+V++G+GGA P+WGG +AK  +   +GG L+ F L K
Sbjct: 534 TEYVAVLAGYGGANPIWGGPMAKIAEHVPRGGTLYVFALKK 574


Lambda     K      H
   0.317    0.137    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1425
Number of extensions: 120
Number of successful extensions: 20
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 595
Length of database: 575
Length adjustment: 37
Effective length of query: 558
Effective length of database: 538
Effective search space:   300204
Effective search space used:   300204
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources