Alignments for a candidate for tpi in Pandoraea thiooxydans ATSB16

GapMind for catabolism of small carbon sources

Alignments for a candidate for tpi in Pandoraea thiooxydans ATSB16

Align Triosephosphate isomerase; TIM; TPI; Triose-phosphate isomerase; EC 5.3.1.1 (characterized)
to candidate WP_047214408.1 PATSB16_RS12290 triose-phosphate isomerase

Query= SwissProt::Q5SJR1
         (250 letters)



>NCBI__GCF_001931675.1:WP_047214408.1
          Length = 259

 Score =  228 bits (582), Expect = 7e-65
 Identities = 122/243 (50%), Positives = 160/243 (65%), Gaps = 4/243 (1%)

Query: 5   LVAGNWKMHKTPSEARVWFAEL--KRLLPPLQSEAAVLPAFPILPVAKEVLAETQVGYGA 62
           LV GNWK+H + +      A L    +L   ++  AV   FP L   +++L  + + +GA
Sbjct: 15  LVVGNWKLHGSLAANAELLARLLASPVLAQGRAVTAVCVPFPYLAQCQQLLGGSPLAWGA 74

Query: 63  QDVSAHKEGAYTGEVSARMLSDLGCRYAIVGHSERRRYHGETDALVAEKAKRLLEEGITP 122
           QDVSA   GA+TGEVSA M+ + G  Y +VGHSERR YHGETDALVA K  R+L  G+TP
Sbjct: 75  QDVSAEINGAFTGEVSAPMIGEFGSSYVLVGHSERRAYHGETDALVAAKTGRVLAAGMTP 134

Query: 123 ILCVGEPLEVREKGEAVPYTLRQLRGSLEGVEPPGPEALVIAYEPVWAIGTGKNATPEDA 182
           I+CVGE L  R+ G+      RQL   L  ++ P    +V+AYEPVWAIGTGK AT E A
Sbjct: 135 IVCVGETLAQRDAGQTDAVVERQLGAVLAALDVPQAARIVVAYEPVWAIGTGKTATREQA 194

Query: 183 EAMHQAIRKALSERYGEAFASRVRILYGGSVNPKNFADLLSMPNVDGGLVGGASLELESF 242
           +A+H+ +R  L+ + GE  A  V+ILYGGSV P N ADL +MP++DGGL+GGASL+ E F
Sbjct: 195 QAVHERLRAQLAAKGGEVAA--VKILYGGSVKPDNAADLFAMPDIDGGLIGGASLKAEDF 252

Query: 243 LAL 245
           LA+
Sbjct: 253 LAI 255


Lambda     K      H
   0.317    0.135    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 206
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 250
Length of database: 259
Length adjustment: 24
Effective length of query: 226
Effective length of database: 235
Effective search space:    53110
Effective search space used:    53110
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

Align candidate WP_047214408.1 PATSB16_RS12290 (triose-phosphate isomerase)
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00419.hmm
# target sequence database:        /tmp/gapView.2158792.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00419  [M=228]
Accession:   TIGR00419
Description: tim: triose-phosphate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
      8e-64  201.6   0.4    9.2e-64  201.4   0.4    1.0  1  NCBI__GCF_001931675.1:WP_047214408.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_001931675.1:WP_047214408.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  201.4   0.4   9.2e-64   9.2e-64       1     228 []      15     250 ..      15     250 .. 0.94

  Alignments for each domain:
  == domain 1  score: 201.4 bits;  conditional E-value: 9.2e-64
                             TIGR00419   1 lviinfKlnesvgkvelevaklae.evaseagvevavappfvdldvvkdeve.seiqvaAqnvdavksGaftG 71 
                                           lv++n+Kl++s+   ++++a+l +  v  +     av +pf +l   ++ +  s +  +Aq+v a   GaftG
  NCBI__GCF_001931675.1:WP_047214408.1  15 LVVGNWKLHGSLAANAELLARLLAsPVLAQGRAVTAVCVPFPYLAQCQQLLGgSPLAWGAQDVSAEINGAFTG 87 
                                           79*******************988567666667779***************9999****************** PP

                             TIGR00419  72 eisAemlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvgetleereaartinnvattaaa 144
                                           e+sA m+ ++G  +vl+gHsErR+++ e+d l+++k  r+ + g++++vCvgetl++r+a++t  +v ++ +a
  NCBI__GCF_001931675.1:WP_047214408.1  88 EVSAPMIGEFGSSYVLVGHSERRAYHGETDALVAAKTGRVLAAGMTPIVCVGETLAQRDAGQTDAVVERQLGA 160
                                           ************************************************************9998888888777 PP

                             TIGR00419 145 aA.......lepdvvAvEPveliGtGkpvskAeaevveksvrdhlkkvskevaesvrvlyGasvtaaedaela 210
                                           +            vvA+EPv++iGtGk++++ +a++v++ +r  l+    eva  v++lyG+sv+  ++a+l+
  NCBI__GCF_001931675.1:WP_047214408.1 161 VLaaldvpqAARIVVAYEPVWAIGTGKTATREQAQAVHERLRAQLAAKGGEVA-AVKILYGGSVKPDNAADLF 232
                                           5336777767789********************************99999*98.6****************** PP

                             TIGR00419 211 aqldvdGvLlasavlkae 228
                                           a +d+dG L+++a+lkae
  NCBI__GCF_001931675.1:WP_047214408.1 233 AMPDIDGGLIGGASLKAE 250
                                           ****************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (228 nodes)
Target sequences:                          1  (259 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 17.34
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources