Alignments for a candidate for ackA in Flavobacterium sp. LM5

GapMind for catabolism of small carbon sources

Alignments for a candidate for ackA in Flavobacterium sp. LM5

Align acetate kinase (EC 2.7.2.1) (characterized)
to candidate WP_078211974.1 BXU11_RS08385 acetate kinase

Query= BRENDA::Q9WYB1
         (403 letters)



>NCBI__GCF_002017945.1:WP_078211974.1
          Length = 398

 Score =  387 bits (993), Expect = e-112
 Identities = 201/401 (50%), Positives = 283/401 (70%), Gaps = 7/401 (1%)

Query: 1   MRVLVINSGSSSIKYQLIEMEGEKVLCKGIAERIGIEGSRLVHRVGDEKHVIERELPDHE 60
           M+V++INSGSSSIKYQLIEM  +KV+C G+ +RIG++ S L +     K V    + +H+
Sbjct: 1   MKVVIINSGSSSIKYQLIEMPEQKVICSGMIDRIGLDTSNLTYTTDANKIVESLPIANHK 60

Query: 61  EALKLILNTLVDEKLGVIKDLKEIDAVGHRVVHGGERFKESVLVDEEVLKAIEEVSPLAP 120
             L+ I   L+D+K+GVI+   EI AVGHRVVHGG  F  +V +  EV + I ++  LAP
Sbjct: 61  IGLQKIAQLLMDDKVGVIQSTSEIKAVGHRVVHGGSSFSNTVEITNEVKEQIRQLIELAP 120

Query: 121 LHNPANLMGIKAAMKLLPGVPNVAVFDTAFHQTIPQKAYLYAIPYEYYEKYKIRRYGFHG 180
           LHNPANL GI  A ++      VAVFDTAFHQT+P +AY YAIP     + KIR YGFHG
Sbjct: 121 LHNPANLEGIVVAEQIFESAKQVAVFDTAFHQTMPVEAYKYAIPNYLLSENKIRAYGFHG 180

Query: 181 TSHRYVSKRAAEILGKKLEEL--KIITCHIGNGASVAAVKYGKCVDTSMGFTPLEGLVMG 238
           TSH+YVS++A E L +K      KIIT H+GNG S+ A+K GK +D ++GF P+ GLVMG
Sbjct: 181 TSHKYVSEKAIEYLTQKANHKADKIITIHLGNGCSMTAIKEGKSIDHTLGFGPMNGLVMG 240

Query: 239 TRSGDLDPAIPFFIMEKEGISPQEMYDILNKKSGVYGLSKGFSSDMRDIEEAALKGDEWC 298
           TRSGD+DP++ F++++  G S  E+  +L K+SG+ GL+ G+ SD+RDIE  A +G++ C
Sbjct: 241 TRSGDVDPSVIFYLIKSLGYSADEVNALLQKQSGMLGLT-GY-SDLRDIESNADEGNKEC 298

Query: 299 KLVLEIYDYRIAKYIGAYAAAMNGVDAIVFTAGVGENSPITREDVCSYLEFLGVKLDKQK 358
           +L L +  YRI K+IGAYAA +NG+DAI+FTAG+GENS + R  VC  +++ G++LD++K
Sbjct: 299 QLALAMNAYRIKKFIGAYAAVLNGLDAIIFTAGIGENSDVIRRLVCEDMDYFGLELDQEK 358

Query: 359 NEETIRGKE-GIISTPDSRVKVLVVPTNEELMIARDTKEIV 398
           N  + R KE   I+TP+++ K+LV+PTNEE+ IA    +++
Sbjct: 359 N--STRSKEIREINTPNAKTKILVIPTNEEVEIANQVFDLL 397


Lambda     K      H
   0.318    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 431
Number of extensions: 10
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 403
Length of database: 398
Length adjustment: 31
Effective length of query: 372
Effective length of database: 367
Effective search space:   136524
Effective search space used:   136524
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

Align candidate WP_078211974.1 BXU11_RS08385 (acetate kinase)
to HMM TIGR00016 (ackA: acetate kinase (EC 2.7.2.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00016.hmm
# target sequence database:        /tmp/gapView.3314241.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00016  [M=405]
Accession:   TIGR00016
Description: ackA: acetate kinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.7e-148  480.6   6.8   1.9e-148  480.4   6.8    1.0  1  NCBI__GCF_002017945.1:WP_078211974.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_002017945.1:WP_078211974.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  480.4   6.8  1.9e-148  1.9e-148       4     403 ..       1     396 [.       1     398 [] 0.96

  Alignments for each domain:
  == domain 1  score: 480.4 bits;  conditional E-value: 1.9e-148
                             TIGR00016   4 kkilvlnaGssslkfalldaensekvllsglverikleeariktvedgekkeeeklaiedheeavkkllntlk 76 
                                           +k++++n+Gsss+k++l+++ + +kv++sg+++ri l+ + ++   ++ +k  e l i++h+ +++k+++ l+
  NCBI__GCF_002017945.1:WP_078211974.1   1 MKVVIINSGSSSIKYQLIEMPE-QKVICSGMIDRIGLDTSNLTYT-TDANKIVESLPIANHKIGLQKIAQLLM 71 
                                           69*******************6.8889*************77655.555889999*****************9 PP

                             TIGR00016  77 k.dkkilkelseialiGHRvvhGgekftesvivtdevlkkikdiselAPlHnpaelegieavlklkvllkakn 148
                                           + +  +++++sei+++GHRvvhGg++f+++v +t+ev ++i++++elAPlHnpa+legi  +   ++ ++ak+
  NCBI__GCF_002017945.1:WP_078211974.1  72 DdKVGVIQSTSEIKAVGHRVVHGGSSFSNTVEITNEVKEQIRQLIELAPLHNPANLEGIVVAE--QIFESAKQ 142
                                           867799*****************************************************9999..7778899* PP

                             TIGR00016 149 vavFDtafHqtipeeaylYalPyslykelgvRrYGfHGtshkyvtqraakllnkpl..ddlnlivcHlGnGas 219
                                           vavFDtafHqt+p eay Ya+P  l  e+++R YGfHGtshkyv+++a ++l +++  +  ++i++HlGnG s
  NCBI__GCF_002017945.1:WP_078211974.1 143 VAVFDTAFHQTMPVEAYKYAIPNYLLSENKIRAYGFHGTSHKYVSEKAIEYLTQKAnhKADKIITIHLGNGCS 215
                                           ****************************************************9887224569*********** PP

                             TIGR00016 220 vsavknGksidtsmGltPLeGlvmGtRsGdiDpaiisylaetlglsldeieetlnkksGllgisglssDlRdi 292
                                           ++a+k+Gksid ++G+ P++GlvmGtRsGd+Dp++i+yl + lg s+de++ +l+k+sG+lg++g  sDlRdi
  NCBI__GCF_002017945.1:WP_078211974.1 216 MTAIKEGKSIDHTLGFGPMNGLVMGTRSGDVDPSVIFYLIKSLGYSADEVNALLQKQSGMLGLTG-YSDLRDI 287
                                           *****************************************************************.89***** PP

                             TIGR00016 293 ldkkeegneeaklAlkvyvhRiakyigkyiaslegelDaivFtgGiGenaaevrelvleklevlGlkldleln 365
                                           +++ +egn+e++lAl + ++Ri+k+ig+y+a l+g lDai+Ft+GiGen+  +r+lv+e+++ +Gl+ld+e+n
  NCBI__GCF_002017945.1:WP_078211974.1 288 ESNADEGNKECQLALAMNAYRIKKFIGAYAAVLNG-LDAIIFTAGIGENSDVIRRLVCEDMDYFGLELDQEKN 359
                                           *********************************88.************************************* PP

                             TIGR00016 366 naarsgkesvisteeskvkvlviptneelviaeDalrl 403
                                             +rs++ + i+t+++k+k+lviptnee+ ia+ ++ l
  NCBI__GCF_002017945.1:WP_078211974.1 360 S-TRSKEIREINTPNAKTKILVIPTNEEVEIANQVFDL 396
                                           9.99999*************************998776 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (405 nodes)
Target sequences:                          1  (398 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 21.82
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources