Finding step gltJ for L-glutamate catabolism in Flavobacterium sp. LM5
No candidates for gltJ: L-glutamate ABC transporter, permease component 1 (gltJ/aatQ)
GapMind classifies a step as low confidence even if it does not find any candidates. You can still try to find candidates by using Curated BLAST (which searches the 6-frame translation) or by text search of the annotations (which may indicate weak homology, under 30% identity or 50% coverage, that GapMind does not consider). See the links below.
Definition of step gltJ
- Curated sequence P0AER3: Glutamate/aspartate import permease protein GltJ. Glutamate/aspartate transport system permease protein GltJ aka B0654, component of Glutamate/aspartate porter. glutamate/aspartate ABC transporter membrane subunit GltJ (EC 7.4.2.1). glutamate/aspartate ABC transporter membrane subunit GltJ (EC 7.4.2.1)
- Curated sequence Q88NY3: PP1070, component of Acidic amino acid uptake porter, AatJMQP
- Curated sequence Pf1N1B4_772: ABC transporter for L-asparagine and L-glutamate, permease component 1
- Curated sequence AO353_16285: ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, permease component 2
- Curated sequence Q9I403: Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida
- Ignore hits to PfGW456L13_4771 when looking for 'other' hits (ABC transporter for L-Asparagine and possibly other L-amino acids, permease component 1)
Or cluster all characterized gltJ proteins
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory